Vitamin C Determination by Ultraviolet Spectroscopy and Multiproduct Calibration
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , |
Tipo de documento: | Relatório |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532016001001912 |
Resumo: | In this work the vitamin C was determined in industrialized nectar juices through ultraviolet (UV) spectroscopy and multiproduct multivariate calibration, based on partial least squares (PLS) regression. Since samples with different flavors, sugar content (light or not) were together in the model construction, it can be considered as a multiproduct and, due to the heterogeneity of the samples, it was necessary to optimize the calibration and validation sets by outliers elimination. The model was developed and validated by the evaluation of the figures of merit such as: accuracy, sensitivity, analytical sensitivity, adjust, linearity, relative prediction deviation, limits of detection and quantification, indicating that the multiproduct model developed from UV spectroscopy and PLS regression can be used in the industrial routine analysis as an alternative to titration or other time and reagent consuming methods. Here, it was evidenced that the UV-PLS multiproduct model provides advantages as being free of sample preparation steps, is suitable to be updated in order to measure other parameters, does not generates residues and is feasible to be implemented for on-line monitoring. Furthermore, the application of multivariate calibration in multiproduct models is extremely attractive from the industrial point of view. |
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Vitamin C Determination by Ultraviolet Spectroscopy and Multiproduct Calibrationvitamin CUV spectroscopymultiproduct calibrationPLSIn this work the vitamin C was determined in industrialized nectar juices through ultraviolet (UV) spectroscopy and multiproduct multivariate calibration, based on partial least squares (PLS) regression. Since samples with different flavors, sugar content (light or not) were together in the model construction, it can be considered as a multiproduct and, due to the heterogeneity of the samples, it was necessary to optimize the calibration and validation sets by outliers elimination. The model was developed and validated by the evaluation of the figures of merit such as: accuracy, sensitivity, analytical sensitivity, adjust, linearity, relative prediction deviation, limits of detection and quantification, indicating that the multiproduct model developed from UV spectroscopy and PLS regression can be used in the industrial routine analysis as an alternative to titration or other time and reagent consuming methods. Here, it was evidenced that the UV-PLS multiproduct model provides advantages as being free of sample preparation steps, is suitable to be updated in order to measure other parameters, does not generates residues and is feasible to be implemented for on-line monitoring. Furthermore, the application of multivariate calibration in multiproduct models is extremely attractive from the industrial point of view.Sociedade Brasileira de Química2016-10-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532016001001912Journal of the Brazilian Chemical Society v.27 n.10 2016reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0103-5053.20160071info:eu-repo/semantics/openAccessSantos,Dayane A.Lima,Karen P.Março,Paulo H.Valderrama,Patríciaeng2016-10-04T00:00:00Zoai:scielo:S0103-50532016001001912Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2016-10-04T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Vitamin C Determination by Ultraviolet Spectroscopy and Multiproduct Calibration |
title |
Vitamin C Determination by Ultraviolet Spectroscopy and Multiproduct Calibration |
spellingShingle |
Vitamin C Determination by Ultraviolet Spectroscopy and Multiproduct Calibration Santos,Dayane A. vitamin C UV spectroscopy multiproduct calibration PLS |
title_short |
Vitamin C Determination by Ultraviolet Spectroscopy and Multiproduct Calibration |
title_full |
Vitamin C Determination by Ultraviolet Spectroscopy and Multiproduct Calibration |
title_fullStr |
Vitamin C Determination by Ultraviolet Spectroscopy and Multiproduct Calibration |
title_full_unstemmed |
Vitamin C Determination by Ultraviolet Spectroscopy and Multiproduct Calibration |
title_sort |
Vitamin C Determination by Ultraviolet Spectroscopy and Multiproduct Calibration |
author |
Santos,Dayane A. |
author_facet |
Santos,Dayane A. Lima,Karen P. Março,Paulo H. Valderrama,Patrícia |
author_role |
author |
author2 |
Lima,Karen P. Março,Paulo H. Valderrama,Patrícia |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Santos,Dayane A. Lima,Karen P. Março,Paulo H. Valderrama,Patrícia |
dc.subject.por.fl_str_mv |
vitamin C UV spectroscopy multiproduct calibration PLS |
topic |
vitamin C UV spectroscopy multiproduct calibration PLS |
description |
In this work the vitamin C was determined in industrialized nectar juices through ultraviolet (UV) spectroscopy and multiproduct multivariate calibration, based on partial least squares (PLS) regression. Since samples with different flavors, sugar content (light or not) were together in the model construction, it can be considered as a multiproduct and, due to the heterogeneity of the samples, it was necessary to optimize the calibration and validation sets by outliers elimination. The model was developed and validated by the evaluation of the figures of merit such as: accuracy, sensitivity, analytical sensitivity, adjust, linearity, relative prediction deviation, limits of detection and quantification, indicating that the multiproduct model developed from UV spectroscopy and PLS regression can be used in the industrial routine analysis as an alternative to titration or other time and reagent consuming methods. Here, it was evidenced that the UV-PLS multiproduct model provides advantages as being free of sample preparation steps, is suitable to be updated in order to measure other parameters, does not generates residues and is feasible to be implemented for on-line monitoring. Furthermore, the application of multivariate calibration in multiproduct models is extremely attractive from the industrial point of view. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/report |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
report |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532016001001912 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532016001001912 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.5935/0103-5053.20160071 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.27 n.10 2016 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318178771140608 |