HPLC/DAD determination of rosmarinic acid in Salvia officinalis: sample preparation optimization by factorial design

Detalhes bibliográficos
Autor(a) principal: Oliveira,Karina B. de
Data de Publicação: 2013
Outros Autores: Oliveira,Brás H. de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000100012
Resumo: Sage (Salvia officinalis) contains high amounts of the biologically active rosmarinic acid (RA) and other polyphenolic compounds. RA is easily oxidized, and may undergo degradation during sample preparation for analysis. The objective of this work was to develop and validate an analytical procedure for determination of RA in sage, using factorial design of experiments for optimizing sample preparation. The statistically significant variables for improving RA extraction yield were determined initially and then used in the optimization step, using central composite design (CCD). The analytical method was then fully validated, and used for the analysis of commercial samples of sage. The optimized procedure involved extraction with aqueous methanol (40%) containing an antioxidant mixture (ascorbic acid and ethylenediaminetetraacetic acid (EDTA)), with sonication at 45 ºC for 20 min. The samples were then injected in a system containing a C18 column, using methanol (A) and 0.1% phosphoric acid in water (B) in step gradient mode (45A:55B, 0-5 min; 80A:20B, 5-10 min) with flow rate of 1.0 mL min-1 and detection at 330 nm. Using this conditions, RA concentrations were 50% higher when compared to extractions without antioxidants (98.94 ± 1.07% recovery). Auto-oxidation of RA during sample extraction was prevented by the use of antioxidants resulting in more reliable analytical results. The method was then used for the analysis of commercial samples of sage.
id SBQ-2_e14758c9c13cfe8c706f9ec5ffadaadf
oai_identifier_str oai:scielo:S0103-50532013000100012
network_acronym_str SBQ-2
network_name_str Journal of the Brazilian Chemical Society (Online)
repository_id_str
spelling HPLC/DAD determination of rosmarinic acid in Salvia officinalis: sample preparation optimization by factorial designSalvia officinallisrosmarinic acidHPLCfactorial design of experimentsSage (Salvia officinalis) contains high amounts of the biologically active rosmarinic acid (RA) and other polyphenolic compounds. RA is easily oxidized, and may undergo degradation during sample preparation for analysis. The objective of this work was to develop and validate an analytical procedure for determination of RA in sage, using factorial design of experiments for optimizing sample preparation. The statistically significant variables for improving RA extraction yield were determined initially and then used in the optimization step, using central composite design (CCD). The analytical method was then fully validated, and used for the analysis of commercial samples of sage. The optimized procedure involved extraction with aqueous methanol (40%) containing an antioxidant mixture (ascorbic acid and ethylenediaminetetraacetic acid (EDTA)), with sonication at 45 ºC for 20 min. The samples were then injected in a system containing a C18 column, using methanol (A) and 0.1% phosphoric acid in water (B) in step gradient mode (45A:55B, 0-5 min; 80A:20B, 5-10 min) with flow rate of 1.0 mL min-1 and detection at 330 nm. Using this conditions, RA concentrations were 50% higher when compared to extractions without antioxidants (98.94 ± 1.07% recovery). Auto-oxidation of RA during sample extraction was prevented by the use of antioxidants resulting in more reliable analytical results. The method was then used for the analysis of commercial samples of sage.Sociedade Brasileira de Química2013-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000100012Journal of the Brazilian Chemical Society v.24 n.1 2013reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532013000100012info:eu-repo/semantics/openAccessOliveira,Karina B. deOliveira,Brás H. deeng2013-02-28T00:00:00Zoai:scielo:S0103-50532013000100012Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2013-02-28T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv HPLC/DAD determination of rosmarinic acid in Salvia officinalis: sample preparation optimization by factorial design
title HPLC/DAD determination of rosmarinic acid in Salvia officinalis: sample preparation optimization by factorial design
spellingShingle HPLC/DAD determination of rosmarinic acid in Salvia officinalis: sample preparation optimization by factorial design
Oliveira,Karina B. de
Salvia officinallis
rosmarinic acid
HPLC
factorial design of experiments
title_short HPLC/DAD determination of rosmarinic acid in Salvia officinalis: sample preparation optimization by factorial design
title_full HPLC/DAD determination of rosmarinic acid in Salvia officinalis: sample preparation optimization by factorial design
title_fullStr HPLC/DAD determination of rosmarinic acid in Salvia officinalis: sample preparation optimization by factorial design
title_full_unstemmed HPLC/DAD determination of rosmarinic acid in Salvia officinalis: sample preparation optimization by factorial design
title_sort HPLC/DAD determination of rosmarinic acid in Salvia officinalis: sample preparation optimization by factorial design
author Oliveira,Karina B. de
author_facet Oliveira,Karina B. de
Oliveira,Brás H. de
author_role author
author2 Oliveira,Brás H. de
author2_role author
dc.contributor.author.fl_str_mv Oliveira,Karina B. de
Oliveira,Brás H. de
dc.subject.por.fl_str_mv Salvia officinallis
rosmarinic acid
HPLC
factorial design of experiments
topic Salvia officinallis
rosmarinic acid
HPLC
factorial design of experiments
description Sage (Salvia officinalis) contains high amounts of the biologically active rosmarinic acid (RA) and other polyphenolic compounds. RA is easily oxidized, and may undergo degradation during sample preparation for analysis. The objective of this work was to develop and validate an analytical procedure for determination of RA in sage, using factorial design of experiments for optimizing sample preparation. The statistically significant variables for improving RA extraction yield were determined initially and then used in the optimization step, using central composite design (CCD). The analytical method was then fully validated, and used for the analysis of commercial samples of sage. The optimized procedure involved extraction with aqueous methanol (40%) containing an antioxidant mixture (ascorbic acid and ethylenediaminetetraacetic acid (EDTA)), with sonication at 45 ºC for 20 min. The samples were then injected in a system containing a C18 column, using methanol (A) and 0.1% phosphoric acid in water (B) in step gradient mode (45A:55B, 0-5 min; 80A:20B, 5-10 min) with flow rate of 1.0 mL min-1 and detection at 330 nm. Using this conditions, RA concentrations were 50% higher when compared to extractions without antioxidants (98.94 ± 1.07% recovery). Auto-oxidation of RA during sample extraction was prevented by the use of antioxidants resulting in more reliable analytical results. The method was then used for the analysis of commercial samples of sage.
publishDate 2013
dc.date.none.fl_str_mv 2013-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000100012
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000100012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-50532013000100012
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.24 n.1 2013
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
_version_ 1750318174439473152