Production of biodiesel catalyzed by Candida rugosa lipase at interface of w/o microemulsion system
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000900022 |
Resumo: | The synthesis between palmitic acid and methanol was carried out in a w/o reverse microemulsion prepared from the mixture of dodecylbenzenesulfonic acid (DBSA)/isooctane/water. Box-Behnken design was adopted to evaluate the effect of significant factors on the methyl palmitate yield and response surface methodology (RSM), which was employed to optimize the process parameters in the esterification. The conditions that showed optimal results for methyl palmitate preparation were: 3.33 w0 ([H2O]/[surfactant]), 4.2 h reaction time, 5:1 methanol/acid molar ratio, and 130 mg g-1 lipase ([lipase]/[acid]) concentration. The following verification experiment obtained a result of 97% in almost total agreement with the expected value (98%). The kinetic constants of the model were determined by experiments at 40 °C with initial concentrations of 0.025-0.25 mol L-1 palmitic acid and 0.025-0.3 mol L-1 methanol in the microemulsion system. The kinetic studies showed that the reaction obeyed the Ping-Pong bi-bi mechanism with inhibition by methanol. |
id |
SBQ-2_e2db23f7a8193e06ee015451d9fd6ef4 |
---|---|
oai_identifier_str |
oai:scielo:S0103-50532014000900022 |
network_acronym_str |
SBQ-2 |
network_name_str |
Journal of the Brazilian Chemical Society (Online) |
repository_id_str |
|
spelling |
Production of biodiesel catalyzed by Candida rugosa lipase at interface of w/o microemulsion systemmicroemulsionlipaseesterificationresponse surface methodology (RSM)inhibitionThe synthesis between palmitic acid and methanol was carried out in a w/o reverse microemulsion prepared from the mixture of dodecylbenzenesulfonic acid (DBSA)/isooctane/water. Box-Behnken design was adopted to evaluate the effect of significant factors on the methyl palmitate yield and response surface methodology (RSM), which was employed to optimize the process parameters in the esterification. The conditions that showed optimal results for methyl palmitate preparation were: 3.33 w0 ([H2O]/[surfactant]), 4.2 h reaction time, 5:1 methanol/acid molar ratio, and 130 mg g-1 lipase ([lipase]/[acid]) concentration. The following verification experiment obtained a result of 97% in almost total agreement with the expected value (98%). The kinetic constants of the model were determined by experiments at 40 °C with initial concentrations of 0.025-0.25 mol L-1 palmitic acid and 0.025-0.3 mol L-1 methanol in the microemulsion system. The kinetic studies showed that the reaction obeyed the Ping-Pong bi-bi mechanism with inhibition by methanol.Sociedade Brasileira de Química2014-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000900022Journal of the Brazilian Chemical Society v.25 n.9 2014reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0103-5053.20140165info:eu-repo/semantics/openAccessTan,ZhongqinFang,MiaoliDu,HuanSong,LelianRen,DahaiTang,XiujuanHan,XiaoxiangLiang,Xinleeng2014-09-30T00:00:00Zoai:scielo:S0103-50532014000900022Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2014-09-30T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Production of biodiesel catalyzed by Candida rugosa lipase at interface of w/o microemulsion system |
title |
Production of biodiesel catalyzed by Candida rugosa lipase at interface of w/o microemulsion system |
spellingShingle |
Production of biodiesel catalyzed by Candida rugosa lipase at interface of w/o microemulsion system Tan,Zhongqin microemulsion lipase esterification response surface methodology (RSM) inhibition |
title_short |
Production of biodiesel catalyzed by Candida rugosa lipase at interface of w/o microemulsion system |
title_full |
Production of biodiesel catalyzed by Candida rugosa lipase at interface of w/o microemulsion system |
title_fullStr |
Production of biodiesel catalyzed by Candida rugosa lipase at interface of w/o microemulsion system |
title_full_unstemmed |
Production of biodiesel catalyzed by Candida rugosa lipase at interface of w/o microemulsion system |
title_sort |
Production of biodiesel catalyzed by Candida rugosa lipase at interface of w/o microemulsion system |
author |
Tan,Zhongqin |
author_facet |
Tan,Zhongqin Fang,Miaoli Du,Huan Song,Lelian Ren,Dahai Tang,Xiujuan Han,Xiaoxiang Liang,Xinle |
author_role |
author |
author2 |
Fang,Miaoli Du,Huan Song,Lelian Ren,Dahai Tang,Xiujuan Han,Xiaoxiang Liang,Xinle |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Tan,Zhongqin Fang,Miaoli Du,Huan Song,Lelian Ren,Dahai Tang,Xiujuan Han,Xiaoxiang Liang,Xinle |
dc.subject.por.fl_str_mv |
microemulsion lipase esterification response surface methodology (RSM) inhibition |
topic |
microemulsion lipase esterification response surface methodology (RSM) inhibition |
description |
The synthesis between palmitic acid and methanol was carried out in a w/o reverse microemulsion prepared from the mixture of dodecylbenzenesulfonic acid (DBSA)/isooctane/water. Box-Behnken design was adopted to evaluate the effect of significant factors on the methyl palmitate yield and response surface methodology (RSM), which was employed to optimize the process parameters in the esterification. The conditions that showed optimal results for methyl palmitate preparation were: 3.33 w0 ([H2O]/[surfactant]), 4.2 h reaction time, 5:1 methanol/acid molar ratio, and 130 mg g-1 lipase ([lipase]/[acid]) concentration. The following verification experiment obtained a result of 97% in almost total agreement with the expected value (98%). The kinetic constants of the model were determined by experiments at 40 °C with initial concentrations of 0.025-0.25 mol L-1 palmitic acid and 0.025-0.3 mol L-1 methanol in the microemulsion system. The kinetic studies showed that the reaction obeyed the Ping-Pong bi-bi mechanism with inhibition by methanol. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000900022 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000900022 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.5935/0103-5053.20140165 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.25 n.9 2014 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318176237780992 |