Production of biodiesel catalyzed by Candida rugosa lipase at interface of w/o microemulsion system

Detalhes bibliográficos
Autor(a) principal: Tan,Zhongqin
Data de Publicação: 2014
Outros Autores: Fang,Miaoli, Du,Huan, Song,Lelian, Ren,Dahai, Tang,Xiujuan, Han,Xiaoxiang, Liang,Xinle
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000900022
Resumo: The synthesis between palmitic acid and methanol was carried out in a w/o reverse microemulsion prepared from the mixture of dodecylbenzenesulfonic acid (DBSA)/isooctane/water. Box-Behnken design was adopted to evaluate the effect of significant factors on the methyl palmitate yield and response surface methodology (RSM), which was employed to optimize the process parameters in the esterification. The conditions that showed optimal results for methyl palmitate preparation were: 3.33 w0 ([H2O]/[surfactant]), 4.2 h reaction time, 5:1 methanol/acid molar ratio, and 130 mg g-1 lipase ([lipase]/[acid]) concentration. The following verification experiment obtained a result of 97% in almost total agreement with the expected value (98%). The kinetic constants of the model were determined by experiments at 40 °C with initial concentrations of 0.025-0.25 mol L-1 palmitic acid and 0.025-0.3 mol L-1 methanol in the microemulsion system. The kinetic studies showed that the reaction obeyed the Ping-Pong bi-bi mechanism with inhibition by methanol.
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spelling Production of biodiesel catalyzed by Candida rugosa lipase at interface of w/o microemulsion systemmicroemulsionlipaseesterificationresponse surface methodology (RSM)inhibitionThe synthesis between palmitic acid and methanol was carried out in a w/o reverse microemulsion prepared from the mixture of dodecylbenzenesulfonic acid (DBSA)/isooctane/water. Box-Behnken design was adopted to evaluate the effect of significant factors on the methyl palmitate yield and response surface methodology (RSM), which was employed to optimize the process parameters in the esterification. The conditions that showed optimal results for methyl palmitate preparation were: 3.33 w0 ([H2O]/[surfactant]), 4.2 h reaction time, 5:1 methanol/acid molar ratio, and 130 mg g-1 lipase ([lipase]/[acid]) concentration. The following verification experiment obtained a result of 97% in almost total agreement with the expected value (98%). The kinetic constants of the model were determined by experiments at 40 °C with initial concentrations of 0.025-0.25 mol L-1 palmitic acid and 0.025-0.3 mol L-1 methanol in the microemulsion system. The kinetic studies showed that the reaction obeyed the Ping-Pong bi-bi mechanism with inhibition by methanol.Sociedade Brasileira de Química2014-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000900022Journal of the Brazilian Chemical Society v.25 n.9 2014reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0103-5053.20140165info:eu-repo/semantics/openAccessTan,ZhongqinFang,MiaoliDu,HuanSong,LelianRen,DahaiTang,XiujuanHan,XiaoxiangLiang,Xinleeng2014-09-30T00:00:00Zoai:scielo:S0103-50532014000900022Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2014-09-30T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Production of biodiesel catalyzed by Candida rugosa lipase at interface of w/o microemulsion system
title Production of biodiesel catalyzed by Candida rugosa lipase at interface of w/o microemulsion system
spellingShingle Production of biodiesel catalyzed by Candida rugosa lipase at interface of w/o microemulsion system
Tan,Zhongqin
microemulsion
lipase
esterification
response surface methodology (RSM)
inhibition
title_short Production of biodiesel catalyzed by Candida rugosa lipase at interface of w/o microemulsion system
title_full Production of biodiesel catalyzed by Candida rugosa lipase at interface of w/o microemulsion system
title_fullStr Production of biodiesel catalyzed by Candida rugosa lipase at interface of w/o microemulsion system
title_full_unstemmed Production of biodiesel catalyzed by Candida rugosa lipase at interface of w/o microemulsion system
title_sort Production of biodiesel catalyzed by Candida rugosa lipase at interface of w/o microemulsion system
author Tan,Zhongqin
author_facet Tan,Zhongqin
Fang,Miaoli
Du,Huan
Song,Lelian
Ren,Dahai
Tang,Xiujuan
Han,Xiaoxiang
Liang,Xinle
author_role author
author2 Fang,Miaoli
Du,Huan
Song,Lelian
Ren,Dahai
Tang,Xiujuan
Han,Xiaoxiang
Liang,Xinle
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Tan,Zhongqin
Fang,Miaoli
Du,Huan
Song,Lelian
Ren,Dahai
Tang,Xiujuan
Han,Xiaoxiang
Liang,Xinle
dc.subject.por.fl_str_mv microemulsion
lipase
esterification
response surface methodology (RSM)
inhibition
topic microemulsion
lipase
esterification
response surface methodology (RSM)
inhibition
description The synthesis between palmitic acid and methanol was carried out in a w/o reverse microemulsion prepared from the mixture of dodecylbenzenesulfonic acid (DBSA)/isooctane/water. Box-Behnken design was adopted to evaluate the effect of significant factors on the methyl palmitate yield and response surface methodology (RSM), which was employed to optimize the process parameters in the esterification. The conditions that showed optimal results for methyl palmitate preparation were: 3.33 w0 ([H2O]/[surfactant]), 4.2 h reaction time, 5:1 methanol/acid molar ratio, and 130 mg g-1 lipase ([lipase]/[acid]) concentration. The following verification experiment obtained a result of 97% in almost total agreement with the expected value (98%). The kinetic constants of the model were determined by experiments at 40 °C with initial concentrations of 0.025-0.25 mol L-1 palmitic acid and 0.025-0.3 mol L-1 methanol in the microemulsion system. The kinetic studies showed that the reaction obeyed the Ping-Pong bi-bi mechanism with inhibition by methanol.
publishDate 2014
dc.date.none.fl_str_mv 2014-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000900022
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000900022
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.5935/0103-5053.20140165
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.25 n.9 2014
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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