Type II photooxidation mechanism of biomolecules using chloro (5,10,15,20-Tetraphenylporphyrinato) indium (III) as a photosensitizer
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000700013 |
Resumo: | The photooxidation mechanism of bovine serum albumin (BSA), L-tryptophan (Trp) and red blood cells (RBC) by chloro(5,10,15,20-tetraphenylporphyrinato)indium(III) (InTPP) was investigated. The photooxidation rate of Trp, BSA and RBC by InTPP was decreased in the presence of NaN3. The presence of D2O increases the photooxidation rate of Trp and BSA and decreases that of RBC. This decrease is probably related to a reduction of the binding constant between InTPP and RBC in the presence of D2O. No significant change in biomolecule fluorescence or in the percent of hemolysis was observed when radical quenchers (ferricyanide, mannitol and dismutase superoxide) were used. Experiments using electron paramagnetic resonance (EPR) show that only ¹O2 was generated by InTPP. A mechanistic model based on the preferential oxidation of Trp and BSA by singlet oxygen is proposed. The agreement between the experimental data and the kinetic model gives additional support to the predominance of a mechanism via ¹O2 in biomolecule photooxidation by InTPP. |
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Type II photooxidation mechanism of biomolecules using chloro (5,10,15,20-Tetraphenylporphyrinato) indium (III) as a photosensitizerchloro(5,10,15,20-tetraphenylporphyrinato)indium(III)photodynamic therapyphotooxidation mechanismcancererythrocytesThe photooxidation mechanism of bovine serum albumin (BSA), L-tryptophan (Trp) and red blood cells (RBC) by chloro(5,10,15,20-tetraphenylporphyrinato)indium(III) (InTPP) was investigated. The photooxidation rate of Trp, BSA and RBC by InTPP was decreased in the presence of NaN3. The presence of D2O increases the photooxidation rate of Trp and BSA and decreases that of RBC. This decrease is probably related to a reduction of the binding constant between InTPP and RBC in the presence of D2O. No significant change in biomolecule fluorescence or in the percent of hemolysis was observed when radical quenchers (ferricyanide, mannitol and dismutase superoxide) were used. Experiments using electron paramagnetic resonance (EPR) show that only ¹O2 was generated by InTPP. A mechanistic model based on the preferential oxidation of Trp and BSA by singlet oxygen is proposed. The agreement between the experimental data and the kinetic model gives additional support to the predominance of a mechanism via ¹O2 in biomolecule photooxidation by InTPP.Sociedade Brasileira de Química2008-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000700013Journal of the Brazilian Chemical Society v.19 n.7 2008reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532008000700013info:eu-repo/semantics/openAccessSilva,André Romero daRibeiro,Joselito NardyRettori,DanielJorge,Renato Atilioeng2017-05-05T00:00:00Zoai:scielo:S0103-50532008000700013Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2017-05-05T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Type II photooxidation mechanism of biomolecules using chloro (5,10,15,20-Tetraphenylporphyrinato) indium (III) as a photosensitizer |
title |
Type II photooxidation mechanism of biomolecules using chloro (5,10,15,20-Tetraphenylporphyrinato) indium (III) as a photosensitizer |
spellingShingle |
Type II photooxidation mechanism of biomolecules using chloro (5,10,15,20-Tetraphenylporphyrinato) indium (III) as a photosensitizer Silva,André Romero da chloro(5,10,15,20-tetraphenylporphyrinato)indium(III) photodynamic therapy photooxidation mechanism cancer erythrocytes |
title_short |
Type II photooxidation mechanism of biomolecules using chloro (5,10,15,20-Tetraphenylporphyrinato) indium (III) as a photosensitizer |
title_full |
Type II photooxidation mechanism of biomolecules using chloro (5,10,15,20-Tetraphenylporphyrinato) indium (III) as a photosensitizer |
title_fullStr |
Type II photooxidation mechanism of biomolecules using chloro (5,10,15,20-Tetraphenylporphyrinato) indium (III) as a photosensitizer |
title_full_unstemmed |
Type II photooxidation mechanism of biomolecules using chloro (5,10,15,20-Tetraphenylporphyrinato) indium (III) as a photosensitizer |
title_sort |
Type II photooxidation mechanism of biomolecules using chloro (5,10,15,20-Tetraphenylporphyrinato) indium (III) as a photosensitizer |
author |
Silva,André Romero da |
author_facet |
Silva,André Romero da Ribeiro,Joselito Nardy Rettori,Daniel Jorge,Renato Atilio |
author_role |
author |
author2 |
Ribeiro,Joselito Nardy Rettori,Daniel Jorge,Renato Atilio |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Silva,André Romero da Ribeiro,Joselito Nardy Rettori,Daniel Jorge,Renato Atilio |
dc.subject.por.fl_str_mv |
chloro(5,10,15,20-tetraphenylporphyrinato)indium(III) photodynamic therapy photooxidation mechanism cancer erythrocytes |
topic |
chloro(5,10,15,20-tetraphenylporphyrinato)indium(III) photodynamic therapy photooxidation mechanism cancer erythrocytes |
description |
The photooxidation mechanism of bovine serum albumin (BSA), L-tryptophan (Trp) and red blood cells (RBC) by chloro(5,10,15,20-tetraphenylporphyrinato)indium(III) (InTPP) was investigated. The photooxidation rate of Trp, BSA and RBC by InTPP was decreased in the presence of NaN3. The presence of D2O increases the photooxidation rate of Trp and BSA and decreases that of RBC. This decrease is probably related to a reduction of the binding constant between InTPP and RBC in the presence of D2O. No significant change in biomolecule fluorescence or in the percent of hemolysis was observed when radical quenchers (ferricyanide, mannitol and dismutase superoxide) were used. Experiments using electron paramagnetic resonance (EPR) show that only ¹O2 was generated by InTPP. A mechanistic model based on the preferential oxidation of Trp and BSA by singlet oxygen is proposed. The agreement between the experimental data and the kinetic model gives additional support to the predominance of a mechanism via ¹O2 in biomolecule photooxidation by InTPP. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000700013 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000700013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-50532008000700013 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.19 n.7 2008 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318169350733824 |