Type II photooxidation mechanism of biomolecules using chloro (5,10,15,20-Tetraphenylporphyrinato) indium (III) as a photosensitizer

Detalhes bibliográficos
Autor(a) principal: Silva,André Romero da
Data de Publicação: 2008
Outros Autores: Ribeiro,Joselito Nardy, Rettori,Daniel, Jorge,Renato Atilio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000700013
Resumo: The photooxidation mechanism of bovine serum albumin (BSA), L-tryptophan (Trp) and red blood cells (RBC) by chloro(5,10,15,20-tetraphenylporphyrinato)indium(III) (InTPP) was investigated. The photooxidation rate of Trp, BSA and RBC by InTPP was decreased in the presence of NaN3. The presence of D2O increases the photooxidation rate of Trp and BSA and decreases that of RBC. This decrease is probably related to a reduction of the binding constant between InTPP and RBC in the presence of D2O. No significant change in biomolecule fluorescence or in the percent of hemolysis was observed when radical quenchers (ferricyanide, mannitol and dismutase superoxide) were used. Experiments using electron paramagnetic resonance (EPR) show that only ¹O2 was generated by InTPP. A mechanistic model based on the preferential oxidation of Trp and BSA by singlet oxygen is proposed. The agreement between the experimental data and the kinetic model gives additional support to the predominance of a mechanism via ¹O2 in biomolecule photooxidation by InTPP.
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spelling Type II photooxidation mechanism of biomolecules using chloro (5,10,15,20-Tetraphenylporphyrinato) indium (III) as a photosensitizerchloro(5,10,15,20-tetraphenylporphyrinato)indium(III)photodynamic therapyphotooxidation mechanismcancererythrocytesThe photooxidation mechanism of bovine serum albumin (BSA), L-tryptophan (Trp) and red blood cells (RBC) by chloro(5,10,15,20-tetraphenylporphyrinato)indium(III) (InTPP) was investigated. The photooxidation rate of Trp, BSA and RBC by InTPP was decreased in the presence of NaN3. The presence of D2O increases the photooxidation rate of Trp and BSA and decreases that of RBC. This decrease is probably related to a reduction of the binding constant between InTPP and RBC in the presence of D2O. No significant change in biomolecule fluorescence or in the percent of hemolysis was observed when radical quenchers (ferricyanide, mannitol and dismutase superoxide) were used. Experiments using electron paramagnetic resonance (EPR) show that only ¹O2 was generated by InTPP. A mechanistic model based on the preferential oxidation of Trp and BSA by singlet oxygen is proposed. The agreement between the experimental data and the kinetic model gives additional support to the predominance of a mechanism via ¹O2 in biomolecule photooxidation by InTPP.Sociedade Brasileira de Química2008-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000700013Journal of the Brazilian Chemical Society v.19 n.7 2008reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532008000700013info:eu-repo/semantics/openAccessSilva,André Romero daRibeiro,Joselito NardyRettori,DanielJorge,Renato Atilioeng2017-05-05T00:00:00Zoai:scielo:S0103-50532008000700013Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2017-05-05T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Type II photooxidation mechanism of biomolecules using chloro (5,10,15,20-Tetraphenylporphyrinato) indium (III) as a photosensitizer
title Type II photooxidation mechanism of biomolecules using chloro (5,10,15,20-Tetraphenylporphyrinato) indium (III) as a photosensitizer
spellingShingle Type II photooxidation mechanism of biomolecules using chloro (5,10,15,20-Tetraphenylporphyrinato) indium (III) as a photosensitizer
Silva,André Romero da
chloro(5,10,15,20-tetraphenylporphyrinato)indium(III)
photodynamic therapy
photooxidation mechanism
cancer
erythrocytes
title_short Type II photooxidation mechanism of biomolecules using chloro (5,10,15,20-Tetraphenylporphyrinato) indium (III) as a photosensitizer
title_full Type II photooxidation mechanism of biomolecules using chloro (5,10,15,20-Tetraphenylporphyrinato) indium (III) as a photosensitizer
title_fullStr Type II photooxidation mechanism of biomolecules using chloro (5,10,15,20-Tetraphenylporphyrinato) indium (III) as a photosensitizer
title_full_unstemmed Type II photooxidation mechanism of biomolecules using chloro (5,10,15,20-Tetraphenylporphyrinato) indium (III) as a photosensitizer
title_sort Type II photooxidation mechanism of biomolecules using chloro (5,10,15,20-Tetraphenylporphyrinato) indium (III) as a photosensitizer
author Silva,André Romero da
author_facet Silva,André Romero da
Ribeiro,Joselito Nardy
Rettori,Daniel
Jorge,Renato Atilio
author_role author
author2 Ribeiro,Joselito Nardy
Rettori,Daniel
Jorge,Renato Atilio
author2_role author
author
author
dc.contributor.author.fl_str_mv Silva,André Romero da
Ribeiro,Joselito Nardy
Rettori,Daniel
Jorge,Renato Atilio
dc.subject.por.fl_str_mv chloro(5,10,15,20-tetraphenylporphyrinato)indium(III)
photodynamic therapy
photooxidation mechanism
cancer
erythrocytes
topic chloro(5,10,15,20-tetraphenylporphyrinato)indium(III)
photodynamic therapy
photooxidation mechanism
cancer
erythrocytes
description The photooxidation mechanism of bovine serum albumin (BSA), L-tryptophan (Trp) and red blood cells (RBC) by chloro(5,10,15,20-tetraphenylporphyrinato)indium(III) (InTPP) was investigated. The photooxidation rate of Trp, BSA and RBC by InTPP was decreased in the presence of NaN3. The presence of D2O increases the photooxidation rate of Trp and BSA and decreases that of RBC. This decrease is probably related to a reduction of the binding constant between InTPP and RBC in the presence of D2O. No significant change in biomolecule fluorescence or in the percent of hemolysis was observed when radical quenchers (ferricyanide, mannitol and dismutase superoxide) were used. Experiments using electron paramagnetic resonance (EPR) show that only ¹O2 was generated by InTPP. A mechanistic model based on the preferential oxidation of Trp and BSA by singlet oxygen is proposed. The agreement between the experimental data and the kinetic model gives additional support to the predominance of a mechanism via ¹O2 in biomolecule photooxidation by InTPP.
publishDate 2008
dc.date.none.fl_str_mv 2008-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532008000700013
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-50532008000700013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.19 n.7 2008
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
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reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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