Evaluation of the Suitability of Analytical Methods in Trichothecene A and B Degradation
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532018001002117 |
Resumo: | An investigation was conducted to standardize conditions for laccase and lipase activity required to maximize trichothecene degradation. The analytical methods were suitable and validated in model solutions for detection of five analytes (T-2 toxin, deoxynivalenol, nivalenol, 3-acetyldeoxynivalenol and 15-acetyldeoxynivalenol), the methods were then applied in the degradation tests of these trichothecenes. The method of salting-out assisted liquid-liquid extraction (SALLE), using three different NaCl concentration levels, obtained recoveries in the range of 47.4-103.4% at pH 5.0 and 36.6-106.8% at pH 7.0, with an intra-day relative standard deviation under 15% for the majority of the compounds. Quantification limits remained in the region of 0.07 µg mL-1 for 15-acetyldeoxynivalenol and 0.3 µg mL-1 for nivalenol. Finally, the suitable analytical methods were applied in a study of trichothecene degradation by enzymatic action, resulting in a reduction of 12.3% for T-2 toxin, 68.4% for deoxynivalenol, 50.2% for 3-acetyldeoxynivalenol and 45.4% for 15-acetyldeoxynivalenol. |
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Evaluation of the Suitability of Analytical Methods in Trichothecene A and B Degradationliquid chromatographyenzymesmycotoxinsbiodegradationAn investigation was conducted to standardize conditions for laccase and lipase activity required to maximize trichothecene degradation. The analytical methods were suitable and validated in model solutions for detection of five analytes (T-2 toxin, deoxynivalenol, nivalenol, 3-acetyldeoxynivalenol and 15-acetyldeoxynivalenol), the methods were then applied in the degradation tests of these trichothecenes. The method of salting-out assisted liquid-liquid extraction (SALLE), using three different NaCl concentration levels, obtained recoveries in the range of 47.4-103.4% at pH 5.0 and 36.6-106.8% at pH 7.0, with an intra-day relative standard deviation under 15% for the majority of the compounds. Quantification limits remained in the region of 0.07 µg mL-1 for 15-acetyldeoxynivalenol and 0.3 µg mL-1 for nivalenol. Finally, the suitable analytical methods were applied in a study of trichothecene degradation by enzymatic action, resulting in a reduction of 12.3% for T-2 toxin, 68.4% for deoxynivalenol, 50.2% for 3-acetyldeoxynivalenol and 45.4% for 15-acetyldeoxynivalenol.Sociedade Brasileira de Química2018-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532018001002117Journal of the Brazilian Chemical Society v.29 n.10 2018reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0103-5053.20180086info:eu-repo/semantics/openAccessFeltrin,Ana Carla P.Sibaja,Karen V. M.Tusnski,CíntiaCaldas,Sergiane S.Primel,Ednei G.Garda-Buffon,Jaquelineeng2018-09-21T00:00:00Zoai:scielo:S0103-50532018001002117Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2018-09-21T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Evaluation of the Suitability of Analytical Methods in Trichothecene A and B Degradation |
title |
Evaluation of the Suitability of Analytical Methods in Trichothecene A and B Degradation |
spellingShingle |
Evaluation of the Suitability of Analytical Methods in Trichothecene A and B Degradation Feltrin,Ana Carla P. liquid chromatography enzymes mycotoxins biodegradation |
title_short |
Evaluation of the Suitability of Analytical Methods in Trichothecene A and B Degradation |
title_full |
Evaluation of the Suitability of Analytical Methods in Trichothecene A and B Degradation |
title_fullStr |
Evaluation of the Suitability of Analytical Methods in Trichothecene A and B Degradation |
title_full_unstemmed |
Evaluation of the Suitability of Analytical Methods in Trichothecene A and B Degradation |
title_sort |
Evaluation of the Suitability of Analytical Methods in Trichothecene A and B Degradation |
author |
Feltrin,Ana Carla P. |
author_facet |
Feltrin,Ana Carla P. Sibaja,Karen V. M. Tusnski,Cíntia Caldas,Sergiane S. Primel,Ednei G. Garda-Buffon,Jaqueline |
author_role |
author |
author2 |
Sibaja,Karen V. M. Tusnski,Cíntia Caldas,Sergiane S. Primel,Ednei G. Garda-Buffon,Jaqueline |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Feltrin,Ana Carla P. Sibaja,Karen V. M. Tusnski,Cíntia Caldas,Sergiane S. Primel,Ednei G. Garda-Buffon,Jaqueline |
dc.subject.por.fl_str_mv |
liquid chromatography enzymes mycotoxins biodegradation |
topic |
liquid chromatography enzymes mycotoxins biodegradation |
description |
An investigation was conducted to standardize conditions for laccase and lipase activity required to maximize trichothecene degradation. The analytical methods were suitable and validated in model solutions for detection of five analytes (T-2 toxin, deoxynivalenol, nivalenol, 3-acetyldeoxynivalenol and 15-acetyldeoxynivalenol), the methods were then applied in the degradation tests of these trichothecenes. The method of salting-out assisted liquid-liquid extraction (SALLE), using three different NaCl concentration levels, obtained recoveries in the range of 47.4-103.4% at pH 5.0 and 36.6-106.8% at pH 7.0, with an intra-day relative standard deviation under 15% for the majority of the compounds. Quantification limits remained in the region of 0.07 µg mL-1 for 15-acetyldeoxynivalenol and 0.3 µg mL-1 for nivalenol. Finally, the suitable analytical methods were applied in a study of trichothecene degradation by enzymatic action, resulting in a reduction of 12.3% for T-2 toxin, 68.4% for deoxynivalenol, 50.2% for 3-acetyldeoxynivalenol and 45.4% for 15-acetyldeoxynivalenol. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532018001002117 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532018001002117 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.21577/0103-5053.20180086 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.29 n.10 2018 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318181218516992 |