A chemometric model applied to fatty acid determination in blood

Detalhes bibliográficos
Autor(a) principal: Lopes,Thiago I. B.
Data de Publicação: 2013
Outros Autores: Rimland,Casey A., Nagassaki,Sabrina, Geloneze,Bruno, Marsaioli,Anita J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013001000007
Resumo: Changes in fatty acid profiles have been associated with several pathophysiological processes. Gas chromatography-mass spectrometry (GC-MS) data monitoring of selected ions was used with principal components analysis (PCA), revealing a set of relevant ions for quantification and characterization of fatty acids. This protocol was successfully applied to the analyses of fatty acids in different human blood lipids, allowing the quantification of several fatty acids and revealing their unsaturation numbers. Moreover the presences of contaminants, artifacts and co-eluitions in the chromatogram were also revealed without any additional analyses. Thus, fatty acid constituents of triglycerides, phospholipids and esters of cholesterol present in plasma and erythrocyte membranes were accurately determined, with repeatability, low limits of determination and of quantification.
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spelling A chemometric model applied to fatty acid determination in bloodfatty acid methyl estersprincipal component analysisselected ion monitoringChanges in fatty acid profiles have been associated with several pathophysiological processes. Gas chromatography-mass spectrometry (GC-MS) data monitoring of selected ions was used with principal components analysis (PCA), revealing a set of relevant ions for quantification and characterization of fatty acids. This protocol was successfully applied to the analyses of fatty acids in different human blood lipids, allowing the quantification of several fatty acids and revealing their unsaturation numbers. Moreover the presences of contaminants, artifacts and co-eluitions in the chromatogram were also revealed without any additional analyses. Thus, fatty acid constituents of triglycerides, phospholipids and esters of cholesterol present in plasma and erythrocyte membranes were accurately determined, with repeatability, low limits of determination and of quantification.Sociedade Brasileira de Química2013-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013001000007Journal of the Brazilian Chemical Society v.24 n.10 2013reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0103-5053.20130201info:eu-repo/semantics/openAccessLopes,Thiago I. B.Rimland,Casey A.Nagassaki,SabrinaGeloneze,BrunoMarsaioli,Anita J.eng2015-07-21T00:00:00Zoai:scielo:S0103-50532013001000007Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2015-07-21T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv A chemometric model applied to fatty acid determination in blood
title A chemometric model applied to fatty acid determination in blood
spellingShingle A chemometric model applied to fatty acid determination in blood
Lopes,Thiago I. B.
fatty acid methyl esters
principal component analysis
selected ion monitoring
title_short A chemometric model applied to fatty acid determination in blood
title_full A chemometric model applied to fatty acid determination in blood
title_fullStr A chemometric model applied to fatty acid determination in blood
title_full_unstemmed A chemometric model applied to fatty acid determination in blood
title_sort A chemometric model applied to fatty acid determination in blood
author Lopes,Thiago I. B.
author_facet Lopes,Thiago I. B.
Rimland,Casey A.
Nagassaki,Sabrina
Geloneze,Bruno
Marsaioli,Anita J.
author_role author
author2 Rimland,Casey A.
Nagassaki,Sabrina
Geloneze,Bruno
Marsaioli,Anita J.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Lopes,Thiago I. B.
Rimland,Casey A.
Nagassaki,Sabrina
Geloneze,Bruno
Marsaioli,Anita J.
dc.subject.por.fl_str_mv fatty acid methyl esters
principal component analysis
selected ion monitoring
topic fatty acid methyl esters
principal component analysis
selected ion monitoring
description Changes in fatty acid profiles have been associated with several pathophysiological processes. Gas chromatography-mass spectrometry (GC-MS) data monitoring of selected ions was used with principal components analysis (PCA), revealing a set of relevant ions for quantification and characterization of fatty acids. This protocol was successfully applied to the analyses of fatty acids in different human blood lipids, allowing the quantification of several fatty acids and revealing their unsaturation numbers. Moreover the presences of contaminants, artifacts and co-eluitions in the chromatogram were also revealed without any additional analyses. Thus, fatty acid constituents of triglycerides, phospholipids and esters of cholesterol present in plasma and erythrocyte membranes were accurately determined, with repeatability, low limits of determination and of quantification.
publishDate 2013
dc.date.none.fl_str_mv 2013-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013001000007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013001000007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.5935/0103-5053.20130201
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.24 n.10 2013
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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