Mixed Ternary Mononuclear Copper(II) Complexes Based on Valproic Acid with 1,10-Phenanthroline and 2,2'-Bipyridine Ligands: DNA Interaction and Cytotoxicity in V79 Cells

Detalhes bibliográficos
Autor(a) principal: Pich,Claus T.
Data de Publicação: 2019
Outros Autores: Santos,Paulo R. dos, Fortunato,Tatiana V. O., Chiarello,Marilda, Oliveira,Iuri M. de, Soares,Bárbara Q., Ghermani,Nour E., Machado,Miriana, Roesch-Ely,Mariana, Dumas,Françoise, Terenzi,Hernan, Henriques,João A. P., Moura,Sidnei
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532019000300597
Resumo: This paper reports the DNA interaction and cytotoxicity of binary and ternary copper(II) complexes of valproic acid with phenanthroline or bipyridine and the complementary characterization of Cu(Valp)2Bipy by crystallography. Circular dichroism, plasmid and oligonucleotide assays have shown that the complexes interact with DNA in different manners and are able to cleave plasmid DNA and oligonucleotides at concentrations from 10 to 1000 mol L-1. The plasmid DNA cleavage is enhanced 650, 375 and 285 times by photo activation with UVB light for the complexes Cu(Valp)2Phen, Cu(Valp)2Bipy and Cu2(Valp)4, respectively. Cytotoxicity assays demonstrated that V79 cells were more sensitive to CuII derivatives than sodium valproate as evaluated by 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) and clonogenic assays, where the cytotoxic profile of the compounds was: Cu(Valp)2Phen higher than Cu(Valp)2Bipy, higher than Cu2(Valp)4. Therefore, we report two copper complexes, which interact with DNA and promote its cleavage, leading to an enhanced cytotoxicity when compared to valproic acid.
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spelling Mixed Ternary Mononuclear Copper(II) Complexes Based on Valproic Acid with 1,10-Phenanthroline and 2,2'-Bipyridine Ligands: DNA Interaction and Cytotoxicity in V79 Cellsorganometallic compoundscopper complexesDNA interactionplasmid cleavageThis paper reports the DNA interaction and cytotoxicity of binary and ternary copper(II) complexes of valproic acid with phenanthroline or bipyridine and the complementary characterization of Cu(Valp)2Bipy by crystallography. Circular dichroism, plasmid and oligonucleotide assays have shown that the complexes interact with DNA in different manners and are able to cleave plasmid DNA and oligonucleotides at concentrations from 10 to 1000 mol L-1. The plasmid DNA cleavage is enhanced 650, 375 and 285 times by photo activation with UVB light for the complexes Cu(Valp)2Phen, Cu(Valp)2Bipy and Cu2(Valp)4, respectively. Cytotoxicity assays demonstrated that V79 cells were more sensitive to CuII derivatives than sodium valproate as evaluated by 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) and clonogenic assays, where the cytotoxic profile of the compounds was: Cu(Valp)2Phen higher than Cu(Valp)2Bipy, higher than Cu2(Valp)4. Therefore, we report two copper complexes, which interact with DNA and promote its cleavage, leading to an enhanced cytotoxicity when compared to valproic acid.Sociedade Brasileira de Química2019-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532019000300597Journal of the Brazilian Chemical Society v.30 n.3 2019reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0103-5053.20180229info:eu-repo/semantics/openAccessPich,Claus T.Santos,Paulo R. dosFortunato,Tatiana V. O.Chiarello,MarildaOliveira,Iuri M. deSoares,Bárbara Q.Ghermani,Nour E.Machado,MirianaRoesch-Ely,MarianaDumas,FrançoiseTerenzi,HernanHenriques,João A. P.Moura,Sidneieng2019-02-14T00:00:00Zoai:scielo:S0103-50532019000300597Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2019-02-14T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Mixed Ternary Mononuclear Copper(II) Complexes Based on Valproic Acid with 1,10-Phenanthroline and 2,2'-Bipyridine Ligands: DNA Interaction and Cytotoxicity in V79 Cells
title Mixed Ternary Mononuclear Copper(II) Complexes Based on Valproic Acid with 1,10-Phenanthroline and 2,2'-Bipyridine Ligands: DNA Interaction and Cytotoxicity in V79 Cells
spellingShingle Mixed Ternary Mononuclear Copper(II) Complexes Based on Valproic Acid with 1,10-Phenanthroline and 2,2'-Bipyridine Ligands: DNA Interaction and Cytotoxicity in V79 Cells
Pich,Claus T.
organometallic compounds
copper complexes
DNA interaction
plasmid cleavage
title_short Mixed Ternary Mononuclear Copper(II) Complexes Based on Valproic Acid with 1,10-Phenanthroline and 2,2'-Bipyridine Ligands: DNA Interaction and Cytotoxicity in V79 Cells
title_full Mixed Ternary Mononuclear Copper(II) Complexes Based on Valproic Acid with 1,10-Phenanthroline and 2,2'-Bipyridine Ligands: DNA Interaction and Cytotoxicity in V79 Cells
title_fullStr Mixed Ternary Mononuclear Copper(II) Complexes Based on Valproic Acid with 1,10-Phenanthroline and 2,2'-Bipyridine Ligands: DNA Interaction and Cytotoxicity in V79 Cells
title_full_unstemmed Mixed Ternary Mononuclear Copper(II) Complexes Based on Valproic Acid with 1,10-Phenanthroline and 2,2'-Bipyridine Ligands: DNA Interaction and Cytotoxicity in V79 Cells
title_sort Mixed Ternary Mononuclear Copper(II) Complexes Based on Valproic Acid with 1,10-Phenanthroline and 2,2'-Bipyridine Ligands: DNA Interaction and Cytotoxicity in V79 Cells
author Pich,Claus T.
author_facet Pich,Claus T.
Santos,Paulo R. dos
Fortunato,Tatiana V. O.
Chiarello,Marilda
Oliveira,Iuri M. de
Soares,Bárbara Q.
Ghermani,Nour E.
Machado,Miriana
Roesch-Ely,Mariana
Dumas,Françoise
Terenzi,Hernan
Henriques,João A. P.
Moura,Sidnei
author_role author
author2 Santos,Paulo R. dos
Fortunato,Tatiana V. O.
Chiarello,Marilda
Oliveira,Iuri M. de
Soares,Bárbara Q.
Ghermani,Nour E.
Machado,Miriana
Roesch-Ely,Mariana
Dumas,Françoise
Terenzi,Hernan
Henriques,João A. P.
Moura,Sidnei
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pich,Claus T.
Santos,Paulo R. dos
Fortunato,Tatiana V. O.
Chiarello,Marilda
Oliveira,Iuri M. de
Soares,Bárbara Q.
Ghermani,Nour E.
Machado,Miriana
Roesch-Ely,Mariana
Dumas,Françoise
Terenzi,Hernan
Henriques,João A. P.
Moura,Sidnei
dc.subject.por.fl_str_mv organometallic compounds
copper complexes
DNA interaction
plasmid cleavage
topic organometallic compounds
copper complexes
DNA interaction
plasmid cleavage
description This paper reports the DNA interaction and cytotoxicity of binary and ternary copper(II) complexes of valproic acid with phenanthroline or bipyridine and the complementary characterization of Cu(Valp)2Bipy by crystallography. Circular dichroism, plasmid and oligonucleotide assays have shown that the complexes interact with DNA in different manners and are able to cleave plasmid DNA and oligonucleotides at concentrations from 10 to 1000 mol L-1. The plasmid DNA cleavage is enhanced 650, 375 and 285 times by photo activation with UVB light for the complexes Cu(Valp)2Phen, Cu(Valp)2Bipy and Cu2(Valp)4, respectively. Cytotoxicity assays demonstrated that V79 cells were more sensitive to CuII derivatives than sodium valproate as evaluated by 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) and clonogenic assays, where the cytotoxic profile of the compounds was: Cu(Valp)2Phen higher than Cu(Valp)2Bipy, higher than Cu2(Valp)4. Therefore, we report two copper complexes, which interact with DNA and promote its cleavage, leading to an enhanced cytotoxicity when compared to valproic acid.
publishDate 2019
dc.date.none.fl_str_mv 2019-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532019000300597
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532019000300597
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.21577/0103-5053.20180229
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.30 n.3 2019
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
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reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
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