Preparation of Magnetoliposomes with a Green, Low-Cost, Fast and Scalable Methodology and Activity Study against S. aureus and C. freundii Bacterial Strains
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532018001202636 |
Resumo: | A novel, fast, low-cost and scalable methodology to prepare stable magnetoliposomes (MGLs), without the use of organic solvents, is described. The concept of the work is based on the dual use of soy lecithin associated to a new liposome preparation methodology. Soy lecithin was used to coat the nanoparticles of magnetite (Fe3 O4 @lecithin) and for encapsulation of Fe3 O4 @lecithin (Lip-Fe3 O4 @lecithin). Liposomes with size less than 160 nm, polydispersity index of 0.25 and zeta potential of -41 mV, were prepared with the use of autoclave and sonication. The liposomal formulations containing magnetite and stigmasterol (Lip-Fe3 O4 @lecithin, Lip-Stigma and Lip-Stigma-Fe3 O4 @lecithin) were shown to be promising for the application as antibacterial. The liposomal formulation and magnetite were characterized by the following techniques: conventional and high-resolution transmission electron microscopy (TEM/HRTEM), energy-filtered transmission electron microscopy (EFTEM), proton nuclear magnetic resonance (1H NMR), Fourier transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRPD), dynamic light scattering (DLS) and zeta potential. The Lip-Fe3 O4 @lecithin had a minimum inhibitory concentration (MIC) of 8.4 µg mL-1 in the presence of 200 Oe magnetic field against S. aureus. |
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Preparation of Magnetoliposomes with a Green, Low-Cost, Fast and Scalable Methodology and Activity Study against S. aureus and C. freundii Bacterial Strainssoy lecithinmagnetiteliposomesS. aureusC. freundiiA novel, fast, low-cost and scalable methodology to prepare stable magnetoliposomes (MGLs), without the use of organic solvents, is described. The concept of the work is based on the dual use of soy lecithin associated to a new liposome preparation methodology. Soy lecithin was used to coat the nanoparticles of magnetite (Fe3 O4 @lecithin) and for encapsulation of Fe3 O4 @lecithin (Lip-Fe3 O4 @lecithin). Liposomes with size less than 160 nm, polydispersity index of 0.25 and zeta potential of -41 mV, were prepared with the use of autoclave and sonication. The liposomal formulations containing magnetite and stigmasterol (Lip-Fe3 O4 @lecithin, Lip-Stigma and Lip-Stigma-Fe3 O4 @lecithin) were shown to be promising for the application as antibacterial. The liposomal formulation and magnetite were characterized by the following techniques: conventional and high-resolution transmission electron microscopy (TEM/HRTEM), energy-filtered transmission electron microscopy (EFTEM), proton nuclear magnetic resonance (1H NMR), Fourier transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRPD), dynamic light scattering (DLS) and zeta potential. The Lip-Fe3 O4 @lecithin had a minimum inhibitory concentration (MIC) of 8.4 µg mL-1 in the presence of 200 Oe magnetic field against S. aureus.Sociedade Brasileira de Química2018-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532018001202636Journal of the Brazilian Chemical Society v.29 n.12 2018reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0103-5053.20180144info:eu-repo/semantics/openAccessSilva,Rosangela M. F. da Costa eLara,Luciano R. S.López,Jorge L.Andrade,Ângela L.Oliveira,Junnia A. C.Takahashi,Jacqueline A.Vieira,Henriete S.Matencio,TulioStumpf,Humberto O.Domingues,Rosana Z.eng2018-11-26T00:00:00Zoai:scielo:S0103-50532018001202636Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2018-11-26T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Preparation of Magnetoliposomes with a Green, Low-Cost, Fast and Scalable Methodology and Activity Study against S. aureus and C. freundii Bacterial Strains |
title |
Preparation of Magnetoliposomes with a Green, Low-Cost, Fast and Scalable Methodology and Activity Study against S. aureus and C. freundii Bacterial Strains |
spellingShingle |
Preparation of Magnetoliposomes with a Green, Low-Cost, Fast and Scalable Methodology and Activity Study against S. aureus and C. freundii Bacterial Strains Silva,Rosangela M. F. da Costa e soy lecithin magnetite liposomes S. aureus C. freundii |
title_short |
Preparation of Magnetoliposomes with a Green, Low-Cost, Fast and Scalable Methodology and Activity Study against S. aureus and C. freundii Bacterial Strains |
title_full |
Preparation of Magnetoliposomes with a Green, Low-Cost, Fast and Scalable Methodology and Activity Study against S. aureus and C. freundii Bacterial Strains |
title_fullStr |
Preparation of Magnetoliposomes with a Green, Low-Cost, Fast and Scalable Methodology and Activity Study against S. aureus and C. freundii Bacterial Strains |
title_full_unstemmed |
Preparation of Magnetoliposomes with a Green, Low-Cost, Fast and Scalable Methodology and Activity Study against S. aureus and C. freundii Bacterial Strains |
title_sort |
Preparation of Magnetoliposomes with a Green, Low-Cost, Fast and Scalable Methodology and Activity Study against S. aureus and C. freundii Bacterial Strains |
author |
Silva,Rosangela M. F. da Costa e |
author_facet |
Silva,Rosangela M. F. da Costa e Lara,Luciano R. S. López,Jorge L. Andrade,Ângela L. Oliveira,Junnia A. C. Takahashi,Jacqueline A. Vieira,Henriete S. Matencio,Tulio Stumpf,Humberto O. Domingues,Rosana Z. |
author_role |
author |
author2 |
Lara,Luciano R. S. López,Jorge L. Andrade,Ângela L. Oliveira,Junnia A. C. Takahashi,Jacqueline A. Vieira,Henriete S. Matencio,Tulio Stumpf,Humberto O. Domingues,Rosana Z. |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva,Rosangela M. F. da Costa e Lara,Luciano R. S. López,Jorge L. Andrade,Ângela L. Oliveira,Junnia A. C. Takahashi,Jacqueline A. Vieira,Henriete S. Matencio,Tulio Stumpf,Humberto O. Domingues,Rosana Z. |
dc.subject.por.fl_str_mv |
soy lecithin magnetite liposomes S. aureus C. freundii |
topic |
soy lecithin magnetite liposomes S. aureus C. freundii |
description |
A novel, fast, low-cost and scalable methodology to prepare stable magnetoliposomes (MGLs), without the use of organic solvents, is described. The concept of the work is based on the dual use of soy lecithin associated to a new liposome preparation methodology. Soy lecithin was used to coat the nanoparticles of magnetite (Fe3 O4 @lecithin) and for encapsulation of Fe3 O4 @lecithin (Lip-Fe3 O4 @lecithin). Liposomes with size less than 160 nm, polydispersity index of 0.25 and zeta potential of -41 mV, were prepared with the use of autoclave and sonication. The liposomal formulations containing magnetite and stigmasterol (Lip-Fe3 O4 @lecithin, Lip-Stigma and Lip-Stigma-Fe3 O4 @lecithin) were shown to be promising for the application as antibacterial. The liposomal formulation and magnetite were characterized by the following techniques: conventional and high-resolution transmission electron microscopy (TEM/HRTEM), energy-filtered transmission electron microscopy (EFTEM), proton nuclear magnetic resonance (1H NMR), Fourier transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRPD), dynamic light scattering (DLS) and zeta potential. The Lip-Fe3 O4 @lecithin had a minimum inhibitory concentration (MIC) of 8.4 µg mL-1 in the presence of 200 Oe magnetic field against S. aureus. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532018001202636 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532018001202636 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.21577/0103-5053.20180144 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.29 n.12 2018 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
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1750318181297160192 |