A new spectrophotometric method for determining the enzymatic activity of endo-β-mannanase in seeds

Detalhes bibliográficos
Autor(a) principal: Pinho,Gevany P.
Data de Publicação: 2014
Outros Autores: Matoso,Juliana R. M., Silvério,Flaviano O., Mota,Welha C., Lopes,Paulo Sérgio N., Ribeiro,Leonardo M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000700013
Resumo: The objective of this study was to develop a new, highly sensitive, efficient and easy touse spectrophotometric method to determine the enzymatic activity of endo-β-mannanase in seeds. The method was optimized and applied to determine the site of enzyme activity in different parts of seeds of Butia capitata. It consists in using the enzyme extracted from seeds to hydrolyze Locust bean gum galactomannan. The reducing sugars react with p-hydroxybenzoic acid hydrazide and are quantified by spectrophotometry in the visible region. The optimized and validated method showed low limits of quantification for the reducing sugars (0.6 µg mannose) and endo-β-mannanase activity (1 mU). Only 5 mg of germinating seeds are needed to perform the method.
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spelling A new spectrophotometric method for determining the enzymatic activity of endo-β-mannanase in seedsgalactomannangerminationreducing sugarThe objective of this study was to develop a new, highly sensitive, efficient and easy touse spectrophotometric method to determine the enzymatic activity of endo-β-mannanase in seeds. The method was optimized and applied to determine the site of enzyme activity in different parts of seeds of Butia capitata. It consists in using the enzyme extracted from seeds to hydrolyze Locust bean gum galactomannan. The reducing sugars react with p-hydroxybenzoic acid hydrazide and are quantified by spectrophotometry in the visible region. The optimized and validated method showed low limits of quantification for the reducing sugars (0.6 µg mannose) and endo-β-mannanase activity (1 mU). Only 5 mg of germinating seeds are needed to perform the method.Sociedade Brasileira de Química2014-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000700013Journal of the Brazilian Chemical Society v.25 n.7 2014reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0103-5053.20140102info:eu-repo/semantics/openAccessPinho,Gevany P.Matoso,Juliana R. M.Silvério,Flaviano O.Mota,Welha C.Lopes,Paulo Sérgio N.Ribeiro,Leonardo M.eng2014-07-17T00:00:00Zoai:scielo:S0103-50532014000700013Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2014-07-17T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv A new spectrophotometric method for determining the enzymatic activity of endo-β-mannanase in seeds
title A new spectrophotometric method for determining the enzymatic activity of endo-β-mannanase in seeds
spellingShingle A new spectrophotometric method for determining the enzymatic activity of endo-β-mannanase in seeds
Pinho,Gevany P.
galactomannan
germination
reducing sugar
title_short A new spectrophotometric method for determining the enzymatic activity of endo-β-mannanase in seeds
title_full A new spectrophotometric method for determining the enzymatic activity of endo-β-mannanase in seeds
title_fullStr A new spectrophotometric method for determining the enzymatic activity of endo-β-mannanase in seeds
title_full_unstemmed A new spectrophotometric method for determining the enzymatic activity of endo-β-mannanase in seeds
title_sort A new spectrophotometric method for determining the enzymatic activity of endo-β-mannanase in seeds
author Pinho,Gevany P.
author_facet Pinho,Gevany P.
Matoso,Juliana R. M.
Silvério,Flaviano O.
Mota,Welha C.
Lopes,Paulo Sérgio N.
Ribeiro,Leonardo M.
author_role author
author2 Matoso,Juliana R. M.
Silvério,Flaviano O.
Mota,Welha C.
Lopes,Paulo Sérgio N.
Ribeiro,Leonardo M.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Pinho,Gevany P.
Matoso,Juliana R. M.
Silvério,Flaviano O.
Mota,Welha C.
Lopes,Paulo Sérgio N.
Ribeiro,Leonardo M.
dc.subject.por.fl_str_mv galactomannan
germination
reducing sugar
topic galactomannan
germination
reducing sugar
description The objective of this study was to develop a new, highly sensitive, efficient and easy touse spectrophotometric method to determine the enzymatic activity of endo-β-mannanase in seeds. The method was optimized and applied to determine the site of enzyme activity in different parts of seeds of Butia capitata. It consists in using the enzyme extracted from seeds to hydrolyze Locust bean gum galactomannan. The reducing sugars react with p-hydroxybenzoic acid hydrazide and are quantified by spectrophotometry in the visible region. The optimized and validated method showed low limits of quantification for the reducing sugars (0.6 µg mannose) and endo-β-mannanase activity (1 mU). Only 5 mg of germinating seeds are needed to perform the method.
publishDate 2014
dc.date.none.fl_str_mv 2014-07-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000700013
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532014000700013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.5935/0103-5053.20140102
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.25 n.7 2014
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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