Lodenafil carbonate tablets: optimization and validation of a capillary zone electrophoresis method

• Autor(a) principal: Codevilla,Cristiane F
• Data de Publicação: 2012
• Outros Autores: Ferreira,Pâmela Cristina L, Sangoi,Maximiliano S, Fröehlich,Pedro Eduardo, Bergold,Ana Maria
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Journal of the Brazilian Chemical Society (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012001100020
• topic: capillary zone electrophoresis; experimental design; lodenafil carbonate; optimization; validation

A simple capillary zone electrophoresis (CZE) method was developed and validated for the analysis of lodenafil carbonate in tablets. Response surface methodology was used for optimization of the pH and concentration of the buffer, applied voltage and temperature. The method employed 50 mmol L-1 borate buffer at pH 10 as background electrolyte with an applied voltage of 15 kV. The separation was carried out in a fused-silica capillary maintained at 32.5 ºC and the detection wavelength was 214 nm. The method was validated showing specificity, linearity (r = 0.9995), precision (relative standard deviation less than 2%) and accuracy (99.95%). The method proved to be robust by a fractional factorial design evaluation. The proposed CZE method was successfully applied for the quantitative analysis of lodenafil carbonate in tablets and the results compared to the high performance liquid chromatography and ultraviolet spectrophotometric methods, showed non-significant differences.