Chromatographic analyses of fatty acid methyl esters by HPLC-UV and GC-FID

Detalhes bibliográficos
Autor(a) principal: Carvalho,Myller S.
Data de Publicação: 2012
Outros Autores: Mendonça,Márcio A., Pinho,David M. M., Resck,Inês S., Suarez,Paulo A. Z.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of the Brazilian Chemical Society (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012000400023
Resumo: An analytical method using high performance liquid chromatography with UV detection (HPLC‑UV) (method A) was used for simultaneous determination of total amounts of triacylglycerides, diacylglycerides, monoacylglycerides and fatty acid methyl esters in alcoholysis of different oil (cotton, canola, sunflower, corn and soybean) samples. Analyses were carried out at 40 °C for 20 min using a gradient of methanol (MeOH) and 2-propanol-hexane 5:4 (v/v) (PrHex): 100% of MeOH in 0 min, 50% of MeOH and 50% of PrHex in 10 min maintained with isocratic elution for 10 min. Another HPLC-UV method (method B) with acetonitrile isocratic elution for 34 min was used to determine the fatty acid composition of oils analyzing their methyl ester derivatives. Contents were determined with satisfactory repeatability (relative standard deviation, RSD < 3%), linearity (r² &gt; 0.99) and sensitivity (limit of quantification). Method B was compared with an official gas chromatographic method with flame ionization detection (GC-FID) from American Oil Chemists' Society (AOCS) in the determination of fatty acid methyl esters (FAME) in biodiesel real samples.
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spelling Chromatographic analyses of fatty acid methyl esters by HPLC-UV and GC-FIDbiodieseltriacylglyceridesgas chromatographyliquid chromatographymethyl esterAn analytical method using high performance liquid chromatography with UV detection (HPLC‑UV) (method A) was used for simultaneous determination of total amounts of triacylglycerides, diacylglycerides, monoacylglycerides and fatty acid methyl esters in alcoholysis of different oil (cotton, canola, sunflower, corn and soybean) samples. Analyses were carried out at 40 °C for 20 min using a gradient of methanol (MeOH) and 2-propanol-hexane 5:4 (v/v) (PrHex): 100% of MeOH in 0 min, 50% of MeOH and 50% of PrHex in 10 min maintained with isocratic elution for 10 min. Another HPLC-UV method (method B) with acetonitrile isocratic elution for 34 min was used to determine the fatty acid composition of oils analyzing their methyl ester derivatives. Contents were determined with satisfactory repeatability (relative standard deviation, RSD < 3%), linearity (r² &gt; 0.99) and sensitivity (limit of quantification). Method B was compared with an official gas chromatographic method with flame ionization detection (GC-FID) from American Oil Chemists' Society (AOCS) in the determination of fatty acid methyl esters (FAME) in biodiesel real samples.Sociedade Brasileira de Química2012-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012000400023Journal of the Brazilian Chemical Society v.23 n.4 2012reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532012000400023info:eu-repo/semantics/openAccessCarvalho,Myller S.Mendonça,Márcio A.Pinho,David M. M.Resck,Inês S.Suarez,Paulo A. Z.eng2012-04-27T00:00:00Zoai:scielo:S0103-50532012000400023Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2012-04-27T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Chromatographic analyses of fatty acid methyl esters by HPLC-UV and GC-FID
title Chromatographic analyses of fatty acid methyl esters by HPLC-UV and GC-FID
spellingShingle Chromatographic analyses of fatty acid methyl esters by HPLC-UV and GC-FID
Carvalho,Myller S.
biodiesel
triacylglycerides
gas chromatography
liquid chromatography
methyl ester
title_short Chromatographic analyses of fatty acid methyl esters by HPLC-UV and GC-FID
title_full Chromatographic analyses of fatty acid methyl esters by HPLC-UV and GC-FID
title_fullStr Chromatographic analyses of fatty acid methyl esters by HPLC-UV and GC-FID
title_full_unstemmed Chromatographic analyses of fatty acid methyl esters by HPLC-UV and GC-FID
title_sort Chromatographic analyses of fatty acid methyl esters by HPLC-UV and GC-FID
author Carvalho,Myller S.
author_facet Carvalho,Myller S.
Mendonça,Márcio A.
Pinho,David M. M.
Resck,Inês S.
Suarez,Paulo A. Z.
author_role author
author2 Mendonça,Márcio A.
Pinho,David M. M.
Resck,Inês S.
Suarez,Paulo A. Z.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Carvalho,Myller S.
Mendonça,Márcio A.
Pinho,David M. M.
Resck,Inês S.
Suarez,Paulo A. Z.
dc.subject.por.fl_str_mv biodiesel
triacylglycerides
gas chromatography
liquid chromatography
methyl ester
topic biodiesel
triacylglycerides
gas chromatography
liquid chromatography
methyl ester
description An analytical method using high performance liquid chromatography with UV detection (HPLC‑UV) (method A) was used for simultaneous determination of total amounts of triacylglycerides, diacylglycerides, monoacylglycerides and fatty acid methyl esters in alcoholysis of different oil (cotton, canola, sunflower, corn and soybean) samples. Analyses were carried out at 40 °C for 20 min using a gradient of methanol (MeOH) and 2-propanol-hexane 5:4 (v/v) (PrHex): 100% of MeOH in 0 min, 50% of MeOH and 50% of PrHex in 10 min maintained with isocratic elution for 10 min. Another HPLC-UV method (method B) with acetonitrile isocratic elution for 34 min was used to determine the fatty acid composition of oils analyzing their methyl ester derivatives. Contents were determined with satisfactory repeatability (relative standard deviation, RSD < 3%), linearity (r² &gt; 0.99) and sensitivity (limit of quantification). Method B was compared with an official gas chromatographic method with flame ionization detection (GC-FID) from American Oil Chemists' Society (AOCS) in the determination of fatty acid methyl esters (FAME) in biodiesel real samples.
publishDate 2012
dc.date.none.fl_str_mv 2012-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012000400023
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012000400023
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-50532012000400023
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Journal of the Brazilian Chemical Society v.23 n.4 2012
reponame:Journal of the Brazilian Chemical Society (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Journal of the Brazilian Chemical Society (Online)
collection Journal of the Brazilian Chemical Society (Online)
repository.name.fl_str_mv Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv ||office@jbcs.sbq.org.br
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