Chromatographic analyses of fatty acid methyl esters by HPLC-UV and GC-FID
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012000400023 |
Resumo: | An analytical method using high performance liquid chromatography with UV detection (HPLC‑UV) (method A) was used for simultaneous determination of total amounts of triacylglycerides, diacylglycerides, monoacylglycerides and fatty acid methyl esters in alcoholysis of different oil (cotton, canola, sunflower, corn and soybean) samples. Analyses were carried out at 40 °C for 20 min using a gradient of methanol (MeOH) and 2-propanol-hexane 5:4 (v/v) (PrHex): 100% of MeOH in 0 min, 50% of MeOH and 50% of PrHex in 10 min maintained with isocratic elution for 10 min. Another HPLC-UV method (method B) with acetonitrile isocratic elution for 34 min was used to determine the fatty acid composition of oils analyzing their methyl ester derivatives. Contents were determined with satisfactory repeatability (relative standard deviation, RSD < 3%), linearity (r² > 0.99) and sensitivity (limit of quantification). Method B was compared with an official gas chromatographic method with flame ionization detection (GC-FID) from American Oil Chemists' Society (AOCS) in the determination of fatty acid methyl esters (FAME) in biodiesel real samples. |
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Chromatographic analyses of fatty acid methyl esters by HPLC-UV and GC-FIDbiodieseltriacylglyceridesgas chromatographyliquid chromatographymethyl esterAn analytical method using high performance liquid chromatography with UV detection (HPLC‑UV) (method A) was used for simultaneous determination of total amounts of triacylglycerides, diacylglycerides, monoacylglycerides and fatty acid methyl esters in alcoholysis of different oil (cotton, canola, sunflower, corn and soybean) samples. Analyses were carried out at 40 °C for 20 min using a gradient of methanol (MeOH) and 2-propanol-hexane 5:4 (v/v) (PrHex): 100% of MeOH in 0 min, 50% of MeOH and 50% of PrHex in 10 min maintained with isocratic elution for 10 min. Another HPLC-UV method (method B) with acetonitrile isocratic elution for 34 min was used to determine the fatty acid composition of oils analyzing their methyl ester derivatives. Contents were determined with satisfactory repeatability (relative standard deviation, RSD < 3%), linearity (r² > 0.99) and sensitivity (limit of quantification). Method B was compared with an official gas chromatographic method with flame ionization detection (GC-FID) from American Oil Chemists' Society (AOCS) in the determination of fatty acid methyl esters (FAME) in biodiesel real samples.Sociedade Brasileira de Química2012-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012000400023Journal of the Brazilian Chemical Society v.23 n.4 2012reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0103-50532012000400023info:eu-repo/semantics/openAccessCarvalho,Myller S.Mendonça,Márcio A.Pinho,David M. M.Resck,Inês S.Suarez,Paulo A. Z.eng2012-04-27T00:00:00Zoai:scielo:S0103-50532012000400023Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2012-04-27T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Chromatographic analyses of fatty acid methyl esters by HPLC-UV and GC-FID |
title |
Chromatographic analyses of fatty acid methyl esters by HPLC-UV and GC-FID |
spellingShingle |
Chromatographic analyses of fatty acid methyl esters by HPLC-UV and GC-FID Carvalho,Myller S. biodiesel triacylglycerides gas chromatography liquid chromatography methyl ester |
title_short |
Chromatographic analyses of fatty acid methyl esters by HPLC-UV and GC-FID |
title_full |
Chromatographic analyses of fatty acid methyl esters by HPLC-UV and GC-FID |
title_fullStr |
Chromatographic analyses of fatty acid methyl esters by HPLC-UV and GC-FID |
title_full_unstemmed |
Chromatographic analyses of fatty acid methyl esters by HPLC-UV and GC-FID |
title_sort |
Chromatographic analyses of fatty acid methyl esters by HPLC-UV and GC-FID |
author |
Carvalho,Myller S. |
author_facet |
Carvalho,Myller S. Mendonça,Márcio A. Pinho,David M. M. Resck,Inês S. Suarez,Paulo A. Z. |
author_role |
author |
author2 |
Mendonça,Márcio A. Pinho,David M. M. Resck,Inês S. Suarez,Paulo A. Z. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Carvalho,Myller S. Mendonça,Márcio A. Pinho,David M. M. Resck,Inês S. Suarez,Paulo A. Z. |
dc.subject.por.fl_str_mv |
biodiesel triacylglycerides gas chromatography liquid chromatography methyl ester |
topic |
biodiesel triacylglycerides gas chromatography liquid chromatography methyl ester |
description |
An analytical method using high performance liquid chromatography with UV detection (HPLC‑UV) (method A) was used for simultaneous determination of total amounts of triacylglycerides, diacylglycerides, monoacylglycerides and fatty acid methyl esters in alcoholysis of different oil (cotton, canola, sunflower, corn and soybean) samples. Analyses were carried out at 40 °C for 20 min using a gradient of methanol (MeOH) and 2-propanol-hexane 5:4 (v/v) (PrHex): 100% of MeOH in 0 min, 50% of MeOH and 50% of PrHex in 10 min maintained with isocratic elution for 10 min. Another HPLC-UV method (method B) with acetonitrile isocratic elution for 34 min was used to determine the fatty acid composition of oils analyzing their methyl ester derivatives. Contents were determined with satisfactory repeatability (relative standard deviation, RSD < 3%), linearity (r² > 0.99) and sensitivity (limit of quantification). Method B was compared with an official gas chromatographic method with flame ionization detection (GC-FID) from American Oil Chemists' Society (AOCS) in the determination of fatty acid methyl esters (FAME) in biodiesel real samples. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012000400023 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532012000400023 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-50532012000400023 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.23 n.4 2012 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
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1750318173172793344 |