Monitorando atividades enzimáticas com sondas fluorogênicas
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Data de Publicação: | 2014 |
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Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Química Nova (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422014000600018 |
Resumo: | Latent fluorogenic probes are essential tools for molecular and chemical biology, providing valuable information about enzymatic activity and occurrence. In this review, a brief outline of fluorophores and latent fluorogenic probes is given. Furthermore, advances and challenges in the development of fluorogenic chemical probes to visualize enzymatic activities (hydrolases and oxidoreductases) of biotechnological and biomedical interest are highlighted, including some methodologies for intracellular imaging. |
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Monitorando atividades enzimáticas com sondas fluorogênicasfluorophoresfluorogenic probesenzymatic assaysLatent fluorogenic probes are essential tools for molecular and chemical biology, providing valuable information about enzymatic activity and occurrence. In this review, a brief outline of fluorophores and latent fluorogenic probes is given. Furthermore, advances and challenges in the development of fluorogenic chemical probes to visualize enzymatic activities (hydrolases and oxidoreductases) of biotechnological and biomedical interest are highlighted, including some methodologies for intracellular imaging.Sociedade Brasileira de Química2014-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422014000600018Química Nova v.37 n.6 2014reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0100-4042.20140142info:eu-repo/semantics/openAccessGonçalves,Caroline da Costa SilvaMarsaioli,Anita Jocelynepor2014-08-01T00:00:00Zoai:scielo:S0100-40422014000600018Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2014-08-01T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Monitorando atividades enzimáticas com sondas fluorogênicas |
title |
Monitorando atividades enzimáticas com sondas fluorogênicas |
spellingShingle |
Monitorando atividades enzimáticas com sondas fluorogênicas Gonçalves,Caroline da Costa Silva fluorophores fluorogenic probes enzymatic assays |
title_short |
Monitorando atividades enzimáticas com sondas fluorogênicas |
title_full |
Monitorando atividades enzimáticas com sondas fluorogênicas |
title_fullStr |
Monitorando atividades enzimáticas com sondas fluorogênicas |
title_full_unstemmed |
Monitorando atividades enzimáticas com sondas fluorogênicas |
title_sort |
Monitorando atividades enzimáticas com sondas fluorogênicas |
author |
Gonçalves,Caroline da Costa Silva |
author_facet |
Gonçalves,Caroline da Costa Silva Marsaioli,Anita Jocelyne |
author_role |
author |
author2 |
Marsaioli,Anita Jocelyne |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Gonçalves,Caroline da Costa Silva Marsaioli,Anita Jocelyne |
dc.subject.por.fl_str_mv |
fluorophores fluorogenic probes enzymatic assays |
topic |
fluorophores fluorogenic probes enzymatic assays |
description |
Latent fluorogenic probes are essential tools for molecular and chemical biology, providing valuable information about enzymatic activity and occurrence. In this review, a brief outline of fluorophores and latent fluorogenic probes is given. Furthermore, advances and challenges in the development of fluorogenic chemical probes to visualize enzymatic activities (hydrolases and oxidoreductases) of biotechnological and biomedical interest are highlighted, including some methodologies for intracellular imaging. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-07-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422014000600018 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422014000600018 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
10.5935/0100-4042.20140142 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Química Nova v.37 n.6 2014 reponame:Química Nova (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Química Nova (Online) |
collection |
Química Nova (Online) |
repository.name.fl_str_mv |
Química Nova (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
quimicanova@sbq.org.br |
_version_ |
1750318115932078080 |