Biochemical properties of Bacillus sp. ITP-001 lipase immobilized with a sol gel process

Detalhes bibliográficos
Autor(a) principal: Carvalho,Nayara Bezerra
Data de Publicação: 2013
Outros Autores: Barbosa,José Murillo P., Oliveira,Maria Vanessa S., Fricks,Alini T., Lima,Álvaro S., Soares,Cleide Mara F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422013000100010
Resumo: This work presents biochemical characterization of a lipase from a new strain of Bacillus sp. ITP-001, immobilized using a sol gel process (IB). The results from the biochemical characterization of IB showed increased activity for hydrolysis, with 526.63 U g-1 at pH 5.0 and 80 ºC, and thermal stability at 37 ºC. Enzymatic activity was stimulated by ions such as EDTA, Fe+3, Mn+2, Zn+2, and Ca+2, and in various organic solvents. Kinetic parameters obtained for the IB were Km = 14.62 mM, and Vmax = 0.102 mM min-1 g-1. The results of biochemical characterization revealed the improved catalytic properties of IB.
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spelling Biochemical properties of Bacillus sp. ITP-001 lipase immobilized with a sol gel processbiochemicallipase from Bacillus immobilizedsol gel processThis work presents biochemical characterization of a lipase from a new strain of Bacillus sp. ITP-001, immobilized using a sol gel process (IB). The results from the biochemical characterization of IB showed increased activity for hydrolysis, with 526.63 U g-1 at pH 5.0 and 80 ºC, and thermal stability at 37 ºC. Enzymatic activity was stimulated by ions such as EDTA, Fe+3, Mn+2, Zn+2, and Ca+2, and in various organic solvents. Kinetic parameters obtained for the IB were Km = 14.62 mM, and Vmax = 0.102 mM min-1 g-1. The results of biochemical characterization revealed the improved catalytic properties of IB.Sociedade Brasileira de Química2013-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422013000100010Química Nova v.36 n.1 2013reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422013000100010info:eu-repo/semantics/openAccessCarvalho,Nayara BezerraBarbosa,José Murillo P.Oliveira,Maria Vanessa S.Fricks,Alini T.Lima,Álvaro S.Soares,Cleide Mara F.eng2013-02-15T00:00:00Zoai:scielo:S0100-40422013000100010Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2013-02-15T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Biochemical properties of Bacillus sp. ITP-001 lipase immobilized with a sol gel process
title Biochemical properties of Bacillus sp. ITP-001 lipase immobilized with a sol gel process
spellingShingle Biochemical properties of Bacillus sp. ITP-001 lipase immobilized with a sol gel process
Carvalho,Nayara Bezerra
biochemical
lipase from Bacillus immobilized
sol gel process
title_short Biochemical properties of Bacillus sp. ITP-001 lipase immobilized with a sol gel process
title_full Biochemical properties of Bacillus sp. ITP-001 lipase immobilized with a sol gel process
title_fullStr Biochemical properties of Bacillus sp. ITP-001 lipase immobilized with a sol gel process
title_full_unstemmed Biochemical properties of Bacillus sp. ITP-001 lipase immobilized with a sol gel process
title_sort Biochemical properties of Bacillus sp. ITP-001 lipase immobilized with a sol gel process
author Carvalho,Nayara Bezerra
author_facet Carvalho,Nayara Bezerra
Barbosa,José Murillo P.
Oliveira,Maria Vanessa S.
Fricks,Alini T.
Lima,Álvaro S.
Soares,Cleide Mara F.
author_role author
author2 Barbosa,José Murillo P.
Oliveira,Maria Vanessa S.
Fricks,Alini T.
Lima,Álvaro S.
Soares,Cleide Mara F.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Carvalho,Nayara Bezerra
Barbosa,José Murillo P.
Oliveira,Maria Vanessa S.
Fricks,Alini T.
Lima,Álvaro S.
Soares,Cleide Mara F.
dc.subject.por.fl_str_mv biochemical
lipase from Bacillus immobilized
sol gel process
topic biochemical
lipase from Bacillus immobilized
sol gel process
description This work presents biochemical characterization of a lipase from a new strain of Bacillus sp. ITP-001, immobilized using a sol gel process (IB). The results from the biochemical characterization of IB showed increased activity for hydrolysis, with 526.63 U g-1 at pH 5.0 and 80 ºC, and thermal stability at 37 ºC. Enzymatic activity was stimulated by ions such as EDTA, Fe+3, Mn+2, Zn+2, and Ca+2, and in various organic solvents. Kinetic parameters obtained for the IB were Km = 14.62 mM, and Vmax = 0.102 mM min-1 g-1. The results of biochemical characterization revealed the improved catalytic properties of IB.
publishDate 2013
dc.date.none.fl_str_mv 2013-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422013000100010
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422013000100010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-40422013000100010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.36 n.1 2013
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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