CROMATOGRAFIA POR INTERAÇÕES HIDROFILICAS (HILIC): ESTADO DA ARTE E APLICAÇÕES

Detalhes bibliográficos
Autor(a) principal: Silva,Carla Grazieli Azevedo da
Data de Publicação: 2016
Outros Autores: Bottoli,Carla Beatriz Grespan, Collins,Carol H.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422016000200210
Resumo: Hydrophilic interaction liquid chromatography (HILIC) has been gaining increased attention for its effective separation of highly polar compounds, which include carbohydrates, amino acids, pharmaceutical compounds, proteins, glycoproteins, nucleosides, etc. Polar compounds are usually poorly retained on reverse-phase liquid chromatography (RP-HPLC) columns or have poor solubility in the apolar mobile phase of normal-phase high performance liquid chromatography (NP-HPLC). Since HILIC uses organic solvents such as ACN or MeOH ( > 70%), also used in RP-HPLC and polar stationary phases similar to NP-HPLC (bare silica, diol, amino, amide, saccharide, zwitterionic stationary phases, etc.), it represents a hybrid of the two separation modes. The high organic content in the MP leads to good compatibility with mass spectrometry (MS), increasing the detectivity. This review describes the fundamentals of HILIC and highlights some interesting applications.
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spelling CROMATOGRAFIA POR INTERAÇÕES HIDROFILICAS (HILIC): ESTADO DA ARTE E APLICAÇÕEShydrophilic interaction chromatographyseparation of polar compoundsHILIC separationshigh performance liquid chromatographypolar stationary phasesHydrophilic interaction liquid chromatography (HILIC) has been gaining increased attention for its effective separation of highly polar compounds, which include carbohydrates, amino acids, pharmaceutical compounds, proteins, glycoproteins, nucleosides, etc. Polar compounds are usually poorly retained on reverse-phase liquid chromatography (RP-HPLC) columns or have poor solubility in the apolar mobile phase of normal-phase high performance liquid chromatography (NP-HPLC). Since HILIC uses organic solvents such as ACN or MeOH ( > 70%), also used in RP-HPLC and polar stationary phases similar to NP-HPLC (bare silica, diol, amino, amide, saccharide, zwitterionic stationary phases, etc.), it represents a hybrid of the two separation modes. The high organic content in the MP leads to good compatibility with mass spectrometry (MS), increasing the detectivity. This review describes the fundamentals of HILIC and highlights some interesting applications.Sociedade Brasileira de Química2016-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422016000200210Química Nova v.39 n.2 2016reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0100-4042.20160003info:eu-repo/semantics/openAccessSilva,Carla Grazieli Azevedo daBottoli,Carla Beatriz GrespanCollins,Carol H.por2016-03-29T00:00:00Zoai:scielo:S0100-40422016000200210Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2016-03-29T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv CROMATOGRAFIA POR INTERAÇÕES HIDROFILICAS (HILIC): ESTADO DA ARTE E APLICAÇÕES
title CROMATOGRAFIA POR INTERAÇÕES HIDROFILICAS (HILIC): ESTADO DA ARTE E APLICAÇÕES
spellingShingle CROMATOGRAFIA POR INTERAÇÕES HIDROFILICAS (HILIC): ESTADO DA ARTE E APLICAÇÕES
Silva,Carla Grazieli Azevedo da
hydrophilic interaction chromatography
separation of polar compounds
HILIC separations
high performance liquid chromatography
polar stationary phases
title_short CROMATOGRAFIA POR INTERAÇÕES HIDROFILICAS (HILIC): ESTADO DA ARTE E APLICAÇÕES
title_full CROMATOGRAFIA POR INTERAÇÕES HIDROFILICAS (HILIC): ESTADO DA ARTE E APLICAÇÕES
title_fullStr CROMATOGRAFIA POR INTERAÇÕES HIDROFILICAS (HILIC): ESTADO DA ARTE E APLICAÇÕES
title_full_unstemmed CROMATOGRAFIA POR INTERAÇÕES HIDROFILICAS (HILIC): ESTADO DA ARTE E APLICAÇÕES
title_sort CROMATOGRAFIA POR INTERAÇÕES HIDROFILICAS (HILIC): ESTADO DA ARTE E APLICAÇÕES
author Silva,Carla Grazieli Azevedo da
author_facet Silva,Carla Grazieli Azevedo da
Bottoli,Carla Beatriz Grespan
Collins,Carol H.
author_role author
author2 Bottoli,Carla Beatriz Grespan
Collins,Carol H.
author2_role author
author
dc.contributor.author.fl_str_mv Silva,Carla Grazieli Azevedo da
Bottoli,Carla Beatriz Grespan
Collins,Carol H.
dc.subject.por.fl_str_mv hydrophilic interaction chromatography
separation of polar compounds
HILIC separations
high performance liquid chromatography
polar stationary phases
topic hydrophilic interaction chromatography
separation of polar compounds
HILIC separations
high performance liquid chromatography
polar stationary phases
description Hydrophilic interaction liquid chromatography (HILIC) has been gaining increased attention for its effective separation of highly polar compounds, which include carbohydrates, amino acids, pharmaceutical compounds, proteins, glycoproteins, nucleosides, etc. Polar compounds are usually poorly retained on reverse-phase liquid chromatography (RP-HPLC) columns or have poor solubility in the apolar mobile phase of normal-phase high performance liquid chromatography (NP-HPLC). Since HILIC uses organic solvents such as ACN or MeOH ( > 70%), also used in RP-HPLC and polar stationary phases similar to NP-HPLC (bare silica, diol, amino, amide, saccharide, zwitterionic stationary phases, etc.), it represents a hybrid of the two separation modes. The high organic content in the MP leads to good compatibility with mass spectrometry (MS), increasing the detectivity. This review describes the fundamentals of HILIC and highlights some interesting applications.
publishDate 2016
dc.date.none.fl_str_mv 2016-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422016000200210
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422016000200210
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv 10.5935/0100-4042.20160003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.39 n.2 2016
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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