CONSTITUINTES QUÍMICOS E ATIVIDADE ANTIOXIDANTE IN VIVO DE FLAVONOIDES ISOLADOS DE Clusia lanceolata (Clusiaceae)

• Autor(a) principal: Ferreira,Rafaela O.
• Data de Publicação: 2016
• Outros Autores: Carvalho Junior,Almir R. de, Riger,Cristiano J., Castro,Rosane N., Silva,Tania M. S. da, Carvalho,Mário G. de
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Química Nova (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422016000901093
• topic: Clusiaceae; Clusia lanceolata; flavonoids; antioxidants; Saccharomyces cerevisiae

The phytochemical investigation of Clusia lanceolata leaves yielded fourteen compounds including six flavones, vitexin, isovitexin, isovitexin-2"-O-α-L-rhamnopyranoside, vitexin-2"-O-α-L-rhamnopyranoside, orientin and isoorientin, two phaeophytins [132-hydroxy-(132-S-)-phaeophytin a and 132-hydroxy-(132-R)-phaeophytin a], two triterpenes, α- and β-amyrin, and four steroids, β-sitosterol, stigmasterol, sitosterone and stigmasterone. Their structures were assigned based on spectroscopic analysis, including two-dimensional NMR techniques and comparison with literature data. The antioxidant capacity in vivo of vitexin and mixing isovitexin-2"-O-α-L-rhamnopyranoside and vitexin-2"-O-α-L-rhamnopyranoside was evaluated using the yeast Saccharomyces cerevisiae as the biological system model and hydrogen peroxide as a stressor agent. The results showed that the tested flavonoids were able to protect the yeast cell against the oxidative damage caused by H2O2.