Quantification of tryptophan in plasma by high performance liquid chromatography
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Química Nova (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012000300032 |
Resumo: | A simple, rapid and selective method using high-performance liquid chromatography with ultraviolet detection (267 nm) was applied for the determination of tryptophan in plasma. Separation was carried out on a C18 column (150 x 4.6 mm internal diameter) in 6 min. The mobile phase consisted of 5 mM the sodium acetate and acetonitrile (92:8, v/v). The method was shown to be precise and accurate, and good recovery of analyte was achieved, characterizing the method as efficient and reliable for use in laboratory analysis. |
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Quantification of tryptophan in plasma by high performance liquid chromatographytryptophanserotoninHPLCA simple, rapid and selective method using high-performance liquid chromatography with ultraviolet detection (267 nm) was applied for the determination of tryptophan in plasma. Separation was carried out on a C18 column (150 x 4.6 mm internal diameter) in 6 min. The mobile phase consisted of 5 mM the sodium acetate and acetonitrile (92:8, v/v). The method was shown to be precise and accurate, and good recovery of analyte was achieved, characterizing the method as efficient and reliable for use in laboratory analysis.Sociedade Brasileira de Química2012-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012000300032Química Nova v.35 n.3 2012reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422012000300032info:eu-repo/semantics/openAccessPinhati,Renata RomanholiPolonini,Hudson CaetanoBrandão,Marcos Antônio FernandesRaposo,Nádia Rezende BarbosaCorchs,FelipeGattaz,Wagner Farideng2012-04-24T00:00:00Zoai:scielo:S0100-40422012000300032Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2012-04-24T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Quantification of tryptophan in plasma by high performance liquid chromatography |
title |
Quantification of tryptophan in plasma by high performance liquid chromatography |
spellingShingle |
Quantification of tryptophan in plasma by high performance liquid chromatography Pinhati,Renata Romanholi tryptophan serotonin HPLC |
title_short |
Quantification of tryptophan in plasma by high performance liquid chromatography |
title_full |
Quantification of tryptophan in plasma by high performance liquid chromatography |
title_fullStr |
Quantification of tryptophan in plasma by high performance liquid chromatography |
title_full_unstemmed |
Quantification of tryptophan in plasma by high performance liquid chromatography |
title_sort |
Quantification of tryptophan in plasma by high performance liquid chromatography |
author |
Pinhati,Renata Romanholi |
author_facet |
Pinhati,Renata Romanholi Polonini,Hudson Caetano Brandão,Marcos Antônio Fernandes Raposo,Nádia Rezende Barbosa Corchs,Felipe Gattaz,Wagner Farid |
author_role |
author |
author2 |
Polonini,Hudson Caetano Brandão,Marcos Antônio Fernandes Raposo,Nádia Rezende Barbosa Corchs,Felipe Gattaz,Wagner Farid |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Pinhati,Renata Romanholi Polonini,Hudson Caetano Brandão,Marcos Antônio Fernandes Raposo,Nádia Rezende Barbosa Corchs,Felipe Gattaz,Wagner Farid |
dc.subject.por.fl_str_mv |
tryptophan serotonin HPLC |
topic |
tryptophan serotonin HPLC |
description |
A simple, rapid and selective method using high-performance liquid chromatography with ultraviolet detection (267 nm) was applied for the determination of tryptophan in plasma. Separation was carried out on a C18 column (150 x 4.6 mm internal diameter) in 6 min. The mobile phase consisted of 5 mM the sodium acetate and acetonitrile (92:8, v/v). The method was shown to be precise and accurate, and good recovery of analyte was achieved, characterizing the method as efficient and reliable for use in laboratory analysis. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012000300032 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012000300032 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-40422012000300032 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Química Nova v.35 n.3 2012 reponame:Química Nova (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Química Nova (Online) |
collection |
Química Nova (Online) |
repository.name.fl_str_mv |
Química Nova (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
quimicanova@sbq.org.br |
_version_ |
1750318113165934592 |