Avaliação da estabilidade do marcador plasmático do estresse oxidativo: malondialdeído

Detalhes bibliográficos
Autor(a) principal: Grotto,Denise
Data de Publicação: 2008
Outros Autores: Valentini,Juliana, Boeira,Silvana, Paniz,Clóvis, Maria,Lucas Santa, Vicentini,Juliana, Moro,Angela, Charão,Mariele, Garcia,Solange Cristina, Cardoso,Simone Gonçalves
Tipo de documento: Artigo
Idioma: por
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422008000200016
Resumo: Malondialdehyde (MDA) is one of the lipid peroxidation products widely used as indicator of cellular injury. However, the short-term and the long-term stability of this biomarker remain unclear. The objective of this work was to evaluate the stability of plasmatic MDA at -20 ºC, utilizing thiobarbituric acid (TBA) as derivative in spectrophotometric and chromatographic analysis. The results showed that MDA was stable for 24 h after blood collection, was not stable when stored after alkaline hydrolysis, remained stable for 30 days after TBA derivatization and was stable for 3 days when stored after n-butanol extraction, all at -20 ºC.
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spelling Avaliação da estabilidade do marcador plasmático do estresse oxidativo: malondialdeídooxidative stressmalondialdehydestabilityMalondialdehyde (MDA) is one of the lipid peroxidation products widely used as indicator of cellular injury. However, the short-term and the long-term stability of this biomarker remain unclear. The objective of this work was to evaluate the stability of plasmatic MDA at -20 ºC, utilizing thiobarbituric acid (TBA) as derivative in spectrophotometric and chromatographic analysis. The results showed that MDA was stable for 24 h after blood collection, was not stable when stored after alkaline hydrolysis, remained stable for 30 days after TBA derivatization and was stable for 3 days when stored after n-butanol extraction, all at -20 ºC.Sociedade Brasileira de Química2008-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422008000200016Química Nova v.31 n.2 2008reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422008000200016info:eu-repo/semantics/openAccessGrotto,DeniseValentini,JulianaBoeira,SilvanaPaniz,ClóvisMaria,Lucas SantaVicentini,JulianaMoro,AngelaCharão,MarieleGarcia,Solange CristinaCardoso,Simone Gonçalvespor2009-09-25T00:00:00Zoai:scielo:S0100-40422008000200016Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2009-09-25T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Avaliação da estabilidade do marcador plasmático do estresse oxidativo: malondialdeído
title Avaliação da estabilidade do marcador plasmático do estresse oxidativo: malondialdeído
spellingShingle Avaliação da estabilidade do marcador plasmático do estresse oxidativo: malondialdeído
Grotto,Denise
oxidative stress
malondialdehyde
stability
title_short Avaliação da estabilidade do marcador plasmático do estresse oxidativo: malondialdeído
title_full Avaliação da estabilidade do marcador plasmático do estresse oxidativo: malondialdeído
title_fullStr Avaliação da estabilidade do marcador plasmático do estresse oxidativo: malondialdeído
title_full_unstemmed Avaliação da estabilidade do marcador plasmático do estresse oxidativo: malondialdeído
title_sort Avaliação da estabilidade do marcador plasmático do estresse oxidativo: malondialdeído
author Grotto,Denise
author_facet Grotto,Denise
Valentini,Juliana
Boeira,Silvana
Paniz,Clóvis
Maria,Lucas Santa
Vicentini,Juliana
Moro,Angela
Charão,Mariele
Garcia,Solange Cristina
Cardoso,Simone Gonçalves
author_role author
author2 Valentini,Juliana
Boeira,Silvana
Paniz,Clóvis
Maria,Lucas Santa
Vicentini,Juliana
Moro,Angela
Charão,Mariele
Garcia,Solange Cristina
Cardoso,Simone Gonçalves
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Grotto,Denise
Valentini,Juliana
Boeira,Silvana
Paniz,Clóvis
Maria,Lucas Santa
Vicentini,Juliana
Moro,Angela
Charão,Mariele
Garcia,Solange Cristina
Cardoso,Simone Gonçalves
dc.subject.por.fl_str_mv oxidative stress
malondialdehyde
stability
topic oxidative stress
malondialdehyde
stability
description Malondialdehyde (MDA) is one of the lipid peroxidation products widely used as indicator of cellular injury. However, the short-term and the long-term stability of this biomarker remain unclear. The objective of this work was to evaluate the stability of plasmatic MDA at -20 ºC, utilizing thiobarbituric acid (TBA) as derivative in spectrophotometric and chromatographic analysis. The results showed that MDA was stable for 24 h after blood collection, was not stable when stored after alkaline hydrolysis, remained stable for 30 days after TBA derivatization and was stable for 3 days when stored after n-butanol extraction, all at -20 ºC.
publishDate 2008
dc.date.none.fl_str_mv 2008-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422008000200016
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422008000200016
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv 10.1590/S0100-40422008000200016
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.31 n.2 2008
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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