Determination of etoricoxib in human plasma using automated on-line solid-phase extraction coupled with LC-APCI/MS/MS

Detalhes bibliográficos
Autor(a) principal: Dalmora,Sérgio Luiz
Data de Publicação: 2008
Outros Autores: Brum Junior,Liberato, Ferretto,Ricardo Machado, Oliveira,Paulo Renato de, Barth,Thiago, Sangoi,Maximiliano da Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422008000300021
Resumo: A liquid chromatography-tandem mass spectrometry method with atmospheric pressure chemical ionization (LC-APCI/MS/MS) was validated for the determination of etoricoxib in human plasma using antipyrin as internal standard, followed by on-line solid-phase extraction. The method was performed on a Luna C18 column and the mobile phase consisted of acetonitrile:water (95:5, v/v)/ammonium acetate (pH 4.0; 10 mM), run at a flow rate of 0.6 mL/min. The method was linear in the range of 1-5000 ng/mL (r²>0.99). The lower limit of quantitation was 1 ng/mL. The recoveries were within 93.72-96.18%. Moreover, method validation demonstrated acceptable results for the precision, accuracy and stability studies.
id SBQ-3_6b02bb44f38fd8e8a90b94243d4fcc69
oai_identifier_str oai:scielo:S0100-40422008000300021
network_acronym_str SBQ-3
network_name_str Química Nova (Online)
repository_id_str
spelling Determination of etoricoxib in human plasma using automated on-line solid-phase extraction coupled with LC-APCI/MS/MSetoricoxibLC-APCI/MS/MSon-line solid-phase extractionA liquid chromatography-tandem mass spectrometry method with atmospheric pressure chemical ionization (LC-APCI/MS/MS) was validated for the determination of etoricoxib in human plasma using antipyrin as internal standard, followed by on-line solid-phase extraction. The method was performed on a Luna C18 column and the mobile phase consisted of acetonitrile:water (95:5, v/v)/ammonium acetate (pH 4.0; 10 mM), run at a flow rate of 0.6 mL/min. The method was linear in the range of 1-5000 ng/mL (r²>0.99). The lower limit of quantitation was 1 ng/mL. The recoveries were within 93.72-96.18%. Moreover, method validation demonstrated acceptable results for the precision, accuracy and stability studies.Sociedade Brasileira de Química2008-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422008000300021Química Nova v.31 n.3 2008reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422008000300021info:eu-repo/semantics/openAccessDalmora,Sérgio LuizBrum Junior,LiberatoFerretto,Ricardo MachadoOliveira,Paulo Renato deBarth,ThiagoSangoi,Maximiliano da Silvaeng2008-06-13T00:00:00Zoai:scielo:S0100-40422008000300021Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2008-06-13T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Determination of etoricoxib in human plasma using automated on-line solid-phase extraction coupled with LC-APCI/MS/MS
title Determination of etoricoxib in human plasma using automated on-line solid-phase extraction coupled with LC-APCI/MS/MS
spellingShingle Determination of etoricoxib in human plasma using automated on-line solid-phase extraction coupled with LC-APCI/MS/MS
Dalmora,Sérgio Luiz
etoricoxib
LC-APCI/MS/MS
on-line solid-phase extraction
title_short Determination of etoricoxib in human plasma using automated on-line solid-phase extraction coupled with LC-APCI/MS/MS
title_full Determination of etoricoxib in human plasma using automated on-line solid-phase extraction coupled with LC-APCI/MS/MS
title_fullStr Determination of etoricoxib in human plasma using automated on-line solid-phase extraction coupled with LC-APCI/MS/MS
title_full_unstemmed Determination of etoricoxib in human plasma using automated on-line solid-phase extraction coupled with LC-APCI/MS/MS
title_sort Determination of etoricoxib in human plasma using automated on-line solid-phase extraction coupled with LC-APCI/MS/MS
author Dalmora,Sérgio Luiz
author_facet Dalmora,Sérgio Luiz
Brum Junior,Liberato
Ferretto,Ricardo Machado
Oliveira,Paulo Renato de
Barth,Thiago
Sangoi,Maximiliano da Silva
author_role author
author2 Brum Junior,Liberato
Ferretto,Ricardo Machado
Oliveira,Paulo Renato de
Barth,Thiago
Sangoi,Maximiliano da Silva
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Dalmora,Sérgio Luiz
Brum Junior,Liberato
Ferretto,Ricardo Machado
Oliveira,Paulo Renato de
Barth,Thiago
Sangoi,Maximiliano da Silva
dc.subject.por.fl_str_mv etoricoxib
LC-APCI/MS/MS
on-line solid-phase extraction
topic etoricoxib
LC-APCI/MS/MS
on-line solid-phase extraction
description A liquid chromatography-tandem mass spectrometry method with atmospheric pressure chemical ionization (LC-APCI/MS/MS) was validated for the determination of etoricoxib in human plasma using antipyrin as internal standard, followed by on-line solid-phase extraction. The method was performed on a Luna C18 column and the mobile phase consisted of acetonitrile:water (95:5, v/v)/ammonium acetate (pH 4.0; 10 mM), run at a flow rate of 0.6 mL/min. The method was linear in the range of 1-5000 ng/mL (r²>0.99). The lower limit of quantitation was 1 ng/mL. The recoveries were within 93.72-96.18%. Moreover, method validation demonstrated acceptable results for the precision, accuracy and stability studies.
publishDate 2008
dc.date.none.fl_str_mv 2008-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422008000300021
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422008000300021
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-40422008000300021
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.31 n.3 2008
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
_version_ 1750318107555004416

Registros relacionados