Development and validation of a method for the analysis of Ochratoxin A in roasted coffee by liquid chromatography/electrospray-mass spectrometry in Tandem (LC/ESI-MS/MS)

• Autor(a) principal: Bandeira,Raquel D. C. C.
• Data de Publicação: 2012
• Outros Autores: Uekane,Thais M., Cunha,Carolina P. da, Geaquinto,Luths R. O., Cunha,Valnei S., Caixeiro,Janaina M. R., Godoy,Ronoel Luiz O., de la Cruz,Marcus Henrique C.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Química Nova (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012000100013
• topic: coffee; Ochratoxin A; LC/ESI-MS/MS

A method using LC/ESI-MS/MS for the quantitative analysis of Ochratoxin A in roasted coffee was described. Linearity was demonstrated (r = 0.9175). The limits of detection and quantification were 1.0 and 3.0 ng g-1, respectively. Trueness, repeatability and intermediate precision values were 89.0-108.8%; 2.4-13.7%; 12.5-17.8%, respectively. To the best of our knowledge, this is the first report in which Ochratoxin A in roasted coffee is analysed by LC/ESI-MS/MS, contributing to the field of mycotoxin analysis, and it will be used for future production of Certified Reference Material.