BIOSSENSORES PLASMÔNICOS BASEADOS EM ESPALHAMENTO RAMAN INTENSIFICADO POR SUPERFÍCIE UTILIZANDO NANOBASTÕES DE OURO
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Química Nova (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422019004901044 |
Resumo: | There is still a significant need for low cost, fast and trustworthy diagnostic devices based on biosensors, which may impact positively for applications in developing countries. Localized surface plasmon resonance (LSPR) based biosensors have been taken as powerful alternative to deal with the above limitations. The local electromagnetic field enhancement caused by the LSPR in metallic nanomaterials allows the emergence of the surface enhanced Raman scattering (SERS) effect, which have recently been applied as biosensing platforms. In the present report, gold nanorods (AuNRs) were synthesized and modified with a sandwich immunoassay to detect bovine serum albumin antibody (anti-BSA). The sensitivity of the platform was evaluated by the extrinsic SERS signal from AuNRs in solution phase with added Nile blue as reporter molecule (AN-S-AuNRs), which was subsequently modified with bovine serum albumin (BSA); the assay consisted on capturing BSA modified AN-S-AuNRs by anti-BSA molecules immobilized on AuNRs on glass slides (AuNRs-chip). SERS mapping evidenced the analyte detection based on the substantial increase in the positive events with SERS signal of the reporter molecule in the presence of anti-BSA compared to the assay in the absence of the antibody. The present study may potentially guide the construction of efficient SERS immunosensors. |
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BIOSSENSORES PLASMÔNICOS BASEADOS EM ESPALHAMENTO RAMAN INTENSIFICADO POR SUPERFÍCIE UTILIZANDO NANOBASTÕES DE OUROgold nanorodsSERS detectionbiosensorsplasmonicsRamanThere is still a significant need for low cost, fast and trustworthy diagnostic devices based on biosensors, which may impact positively for applications in developing countries. Localized surface plasmon resonance (LSPR) based biosensors have been taken as powerful alternative to deal with the above limitations. The local electromagnetic field enhancement caused by the LSPR in metallic nanomaterials allows the emergence of the surface enhanced Raman scattering (SERS) effect, which have recently been applied as biosensing platforms. In the present report, gold nanorods (AuNRs) were synthesized and modified with a sandwich immunoassay to detect bovine serum albumin antibody (anti-BSA). The sensitivity of the platform was evaluated by the extrinsic SERS signal from AuNRs in solution phase with added Nile blue as reporter molecule (AN-S-AuNRs), which was subsequently modified with bovine serum albumin (BSA); the assay consisted on capturing BSA modified AN-S-AuNRs by anti-BSA molecules immobilized on AuNRs on glass slides (AuNRs-chip). SERS mapping evidenced the analyte detection based on the substantial increase in the positive events with SERS signal of the reporter molecule in the presence of anti-BSA compared to the assay in the absence of the antibody. The present study may potentially guide the construction of efficient SERS immunosensors.Sociedade Brasileira de Química2019-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422019004901044Química Nova v.42 n.9 2019reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0100-4042.20170416info:eu-repo/semantics/openAccessPeixoto,Linus Pauling F.Santos,Jacqueline F. L.Andrade,Gustavo F. S.por2019-12-09T00:00:00Zoai:scielo:S0100-40422019004901044Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2019-12-09T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
BIOSSENSORES PLASMÔNICOS BASEADOS EM ESPALHAMENTO RAMAN INTENSIFICADO POR SUPERFÍCIE UTILIZANDO NANOBASTÕES DE OURO |
title |
BIOSSENSORES PLASMÔNICOS BASEADOS EM ESPALHAMENTO RAMAN INTENSIFICADO POR SUPERFÍCIE UTILIZANDO NANOBASTÕES DE OURO |
spellingShingle |
BIOSSENSORES PLASMÔNICOS BASEADOS EM ESPALHAMENTO RAMAN INTENSIFICADO POR SUPERFÍCIE UTILIZANDO NANOBASTÕES DE OURO Peixoto,Linus Pauling F. gold nanorods SERS detection biosensors plasmonics Raman |
title_short |
BIOSSENSORES PLASMÔNICOS BASEADOS EM ESPALHAMENTO RAMAN INTENSIFICADO POR SUPERFÍCIE UTILIZANDO NANOBASTÕES DE OURO |
title_full |
BIOSSENSORES PLASMÔNICOS BASEADOS EM ESPALHAMENTO RAMAN INTENSIFICADO POR SUPERFÍCIE UTILIZANDO NANOBASTÕES DE OURO |
title_fullStr |
BIOSSENSORES PLASMÔNICOS BASEADOS EM ESPALHAMENTO RAMAN INTENSIFICADO POR SUPERFÍCIE UTILIZANDO NANOBASTÕES DE OURO |
title_full_unstemmed |
BIOSSENSORES PLASMÔNICOS BASEADOS EM ESPALHAMENTO RAMAN INTENSIFICADO POR SUPERFÍCIE UTILIZANDO NANOBASTÕES DE OURO |
title_sort |
BIOSSENSORES PLASMÔNICOS BASEADOS EM ESPALHAMENTO RAMAN INTENSIFICADO POR SUPERFÍCIE UTILIZANDO NANOBASTÕES DE OURO |
author |
Peixoto,Linus Pauling F. |
author_facet |
Peixoto,Linus Pauling F. Santos,Jacqueline F. L. Andrade,Gustavo F. S. |
author_role |
author |
author2 |
Santos,Jacqueline F. L. Andrade,Gustavo F. S. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Peixoto,Linus Pauling F. Santos,Jacqueline F. L. Andrade,Gustavo F. S. |
dc.subject.por.fl_str_mv |
gold nanorods SERS detection biosensors plasmonics Raman |
topic |
gold nanorods SERS detection biosensors plasmonics Raman |
description |
There is still a significant need for low cost, fast and trustworthy diagnostic devices based on biosensors, which may impact positively for applications in developing countries. Localized surface plasmon resonance (LSPR) based biosensors have been taken as powerful alternative to deal with the above limitations. The local electromagnetic field enhancement caused by the LSPR in metallic nanomaterials allows the emergence of the surface enhanced Raman scattering (SERS) effect, which have recently been applied as biosensing platforms. In the present report, gold nanorods (AuNRs) were synthesized and modified with a sandwich immunoassay to detect bovine serum albumin antibody (anti-BSA). The sensitivity of the platform was evaluated by the extrinsic SERS signal from AuNRs in solution phase with added Nile blue as reporter molecule (AN-S-AuNRs), which was subsequently modified with bovine serum albumin (BSA); the assay consisted on capturing BSA modified AN-S-AuNRs by anti-BSA molecules immobilized on AuNRs on glass slides (AuNRs-chip). SERS mapping evidenced the analyte detection based on the substantial increase in the positive events with SERS signal of the reporter molecule in the presence of anti-BSA compared to the assay in the absence of the antibody. The present study may potentially guide the construction of efficient SERS immunosensors. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422019004901044 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422019004901044 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
10.21577/0100-4042.20170416 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Química Nova v.42 n.9 2019 reponame:Química Nova (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Química Nova (Online) |
collection |
Química Nova (Online) |
repository.name.fl_str_mv |
Química Nova (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
quimicanova@sbq.org.br |
_version_ |
1750318120046690304 |