INVESTIGAÇÃO DA SUPRESSÃO DE FLUORESCÊNCIA DE SORO ALBUMINA BOVINA E HUMANA POR COMPLEXO DE RUTÊNIO
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Publication Date: | 2015 |
Other Authors: | , , , , |
Format: | Article |
Language: | por |
Source: | Química Nova (Online) |
Download full: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422015000200227 |
Summary: | The binding of [RuCl2(L)] (L = N,N-bis(7-methyl-2-pyridylmethylene)-1,3-diiminopropane) to bovine and human serum albumin was investigated by the fluorescence quenching technique. The comparison of the quenching effect of serum albumin fluorescence by ruthenium complex allowed the estimation of subdomain IB in BSA and subdomain IIA in HSA as the binding sites for this complex. The results of fluorescence titration revealed that ruthenium complex quenches the intrinsic fluorescence of BSA through a dynamic quenching mechanism, while HSA has a static quenching mechanism. The thermodynamic parameters indicated that hydrophobic forces played a major role in the binding of ruthenium complex to proteins. The process of binding was a spontaneous process in which Gibbs free energy change was negative. |
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INVESTIGAÇÃO DA SUPRESSÃO DE FLUORESCÊNCIA DE SORO ALBUMINA BOVINA E HUMANA POR COMPLEXO DE RUTÊNIOserum albuminrutheniumdynamic quenchingstatic quenchingThe binding of [RuCl2(L)] (L = N,N-bis(7-methyl-2-pyridylmethylene)-1,3-diiminopropane) to bovine and human serum albumin was investigated by the fluorescence quenching technique. The comparison of the quenching effect of serum albumin fluorescence by ruthenium complex allowed the estimation of subdomain IB in BSA and subdomain IIA in HSA as the binding sites for this complex. The results of fluorescence titration revealed that ruthenium complex quenches the intrinsic fluorescence of BSA through a dynamic quenching mechanism, while HSA has a static quenching mechanism. The thermodynamic parameters indicated that hydrophobic forces played a major role in the binding of ruthenium complex to proteins. The process of binding was a spontaneous process in which Gibbs free energy change was negative.Sociedade Brasileira de Química2015-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422015000200227Química Nova v.38 n.2 2015reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0100-4042.20140315info:eu-repo/semantics/openAccessMoreira,Mariete B.Franciscato,Douglas S.Toledo,Kalil C. F.Souza,João Raul B. deNakatani,Helena S.Souza,Vagner R. depor2015-04-09T00:00:00Zoai:scielo:S0100-40422015000200227Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2015-04-09T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
INVESTIGAÇÃO DA SUPRESSÃO DE FLUORESCÊNCIA DE SORO ALBUMINA BOVINA E HUMANA POR COMPLEXO DE RUTÊNIO |
title |
INVESTIGAÇÃO DA SUPRESSÃO DE FLUORESCÊNCIA DE SORO ALBUMINA BOVINA E HUMANA POR COMPLEXO DE RUTÊNIO |
spellingShingle |
INVESTIGAÇÃO DA SUPRESSÃO DE FLUORESCÊNCIA DE SORO ALBUMINA BOVINA E HUMANA POR COMPLEXO DE RUTÊNIO Moreira,Mariete B. serum albumin ruthenium dynamic quenching static quenching |
title_short |
INVESTIGAÇÃO DA SUPRESSÃO DE FLUORESCÊNCIA DE SORO ALBUMINA BOVINA E HUMANA POR COMPLEXO DE RUTÊNIO |
title_full |
INVESTIGAÇÃO DA SUPRESSÃO DE FLUORESCÊNCIA DE SORO ALBUMINA BOVINA E HUMANA POR COMPLEXO DE RUTÊNIO |
title_fullStr |
INVESTIGAÇÃO DA SUPRESSÃO DE FLUORESCÊNCIA DE SORO ALBUMINA BOVINA E HUMANA POR COMPLEXO DE RUTÊNIO |
title_full_unstemmed |
INVESTIGAÇÃO DA SUPRESSÃO DE FLUORESCÊNCIA DE SORO ALBUMINA BOVINA E HUMANA POR COMPLEXO DE RUTÊNIO |
title_sort |
INVESTIGAÇÃO DA SUPRESSÃO DE FLUORESCÊNCIA DE SORO ALBUMINA BOVINA E HUMANA POR COMPLEXO DE RUTÊNIO |
author |
Moreira,Mariete B. |
author_facet |
Moreira,Mariete B. Franciscato,Douglas S. Toledo,Kalil C. F. Souza,João Raul B. de Nakatani,Helena S. Souza,Vagner R. de |
author_role |
author |
author2 |
Franciscato,Douglas S. Toledo,Kalil C. F. Souza,João Raul B. de Nakatani,Helena S. Souza,Vagner R. de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Moreira,Mariete B. Franciscato,Douglas S. Toledo,Kalil C. F. Souza,João Raul B. de Nakatani,Helena S. Souza,Vagner R. de |
dc.subject.por.fl_str_mv |
serum albumin ruthenium dynamic quenching static quenching |
topic |
serum albumin ruthenium dynamic quenching static quenching |
description |
The binding of [RuCl2(L)] (L = N,N-bis(7-methyl-2-pyridylmethylene)-1,3-diiminopropane) to bovine and human serum albumin was investigated by the fluorescence quenching technique. The comparison of the quenching effect of serum albumin fluorescence by ruthenium complex allowed the estimation of subdomain IB in BSA and subdomain IIA in HSA as the binding sites for this complex. The results of fluorescence titration revealed that ruthenium complex quenches the intrinsic fluorescence of BSA through a dynamic quenching mechanism, while HSA has a static quenching mechanism. The thermodynamic parameters indicated that hydrophobic forces played a major role in the binding of ruthenium complex to proteins. The process of binding was a spontaneous process in which Gibbs free energy change was negative. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422015000200227 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422015000200227 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
10.5935/0100-4042.20140315 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Química Nova v.38 n.2 2015 reponame:Química Nova (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Química Nova (Online) |
collection |
Química Nova (Online) |
repository.name.fl_str_mv |
Química Nova (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
quimicanova@sbq.org.br |
_version_ |
1750318116647206912 |