Atividade inibitória de pesticidas piretroides sobre a glutationa S-transferase
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Química Nova (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422022000200138 |
Resumo: | Glutathione S-transferase (GST) is one of the enzymes used to evaluate the effects caused by environmental contaminants. The aim of this work was to study the effects of four pyrethroid compounds (allethrin, cypermethrin, deltamethrin and fenpropathrin) on the catalytic activity of GST and to estimate the potential for environmental contamination in silico of these insecticides. Spectrophotometric analyses showed that the activity of the GST enzyme was inhibited by the four pesticides, with the values for the inhibition constant (Ki) varying from 0.12 to 20 µmol L-1, with the following order of inhibition from the highest to the lowest Ki value: deltamethrin > cypermethrin > allethrin > fenpropathrin. They presented a competitive and non-competitive inhibition mechanism depending on the compound. The IC50 values (concentration that inhibits 50% of the enzyme activity) showed the insecticide deltamethrin with greater inhibitory capacity, since it reduced the activity of the GST enzyme by half, even though it was in a lower concentration in relation to the other analyzed compounds. The in silico evaluation indicated the possibility of most of the insecticides contaminating the surface waters (rivers and lakes) adhered to the suspended sediment, but did not show any potential for contamination of groundwater through leaching. |
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Atividade inibitória de pesticidas piretroides sobre a glutationa S-transferasesynthetic pesticidesenzymatic activityspectrophotometryin silicoenvironmental contaminationGlutathione S-transferase (GST) is one of the enzymes used to evaluate the effects caused by environmental contaminants. The aim of this work was to study the effects of four pyrethroid compounds (allethrin, cypermethrin, deltamethrin and fenpropathrin) on the catalytic activity of GST and to estimate the potential for environmental contamination in silico of these insecticides. Spectrophotometric analyses showed that the activity of the GST enzyme was inhibited by the four pesticides, with the values for the inhibition constant (Ki) varying from 0.12 to 20 µmol L-1, with the following order of inhibition from the highest to the lowest Ki value: deltamethrin > cypermethrin > allethrin > fenpropathrin. They presented a competitive and non-competitive inhibition mechanism depending on the compound. The IC50 values (concentration that inhibits 50% of the enzyme activity) showed the insecticide deltamethrin with greater inhibitory capacity, since it reduced the activity of the GST enzyme by half, even though it was in a lower concentration in relation to the other analyzed compounds. The in silico evaluation indicated the possibility of most of the insecticides contaminating the surface waters (rivers and lakes) adhered to the suspended sediment, but did not show any potential for contamination of groundwater through leaching.Sociedade Brasileira de Química2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422022000200138Química Nova v.45 n.2 2022reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0100-4042.20170804info:eu-repo/semantics/openAccessRibeiro,Eliane BBadea,MihaelaRibeiro,Danilo BCosta,Andressa R. CSantos,Djanira R. dosMarques,Paulo R. B. ONunes,Gilvanda Spor2022-04-18T00:00:00Zoai:scielo:S0100-40422022000200138Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2022-04-18T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Atividade inibitória de pesticidas piretroides sobre a glutationa S-transferase |
title |
Atividade inibitória de pesticidas piretroides sobre a glutationa S-transferase |
spellingShingle |
Atividade inibitória de pesticidas piretroides sobre a glutationa S-transferase Ribeiro,Eliane B synthetic pesticides enzymatic activity spectrophotometry in silico environmental contamination |
title_short |
Atividade inibitória de pesticidas piretroides sobre a glutationa S-transferase |
title_full |
Atividade inibitória de pesticidas piretroides sobre a glutationa S-transferase |
title_fullStr |
Atividade inibitória de pesticidas piretroides sobre a glutationa S-transferase |
title_full_unstemmed |
Atividade inibitória de pesticidas piretroides sobre a glutationa S-transferase |
title_sort |
Atividade inibitória de pesticidas piretroides sobre a glutationa S-transferase |
author |
Ribeiro,Eliane B |
author_facet |
Ribeiro,Eliane B Badea,Mihaela Ribeiro,Danilo B Costa,Andressa R. C Santos,Djanira R. dos Marques,Paulo R. B. O Nunes,Gilvanda S |
author_role |
author |
author2 |
Badea,Mihaela Ribeiro,Danilo B Costa,Andressa R. C Santos,Djanira R. dos Marques,Paulo R. B. O Nunes,Gilvanda S |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Ribeiro,Eliane B Badea,Mihaela Ribeiro,Danilo B Costa,Andressa R. C Santos,Djanira R. dos Marques,Paulo R. B. O Nunes,Gilvanda S |
dc.subject.por.fl_str_mv |
synthetic pesticides enzymatic activity spectrophotometry in silico environmental contamination |
topic |
synthetic pesticides enzymatic activity spectrophotometry in silico environmental contamination |
description |
Glutathione S-transferase (GST) is one of the enzymes used to evaluate the effects caused by environmental contaminants. The aim of this work was to study the effects of four pyrethroid compounds (allethrin, cypermethrin, deltamethrin and fenpropathrin) on the catalytic activity of GST and to estimate the potential for environmental contamination in silico of these insecticides. Spectrophotometric analyses showed that the activity of the GST enzyme was inhibited by the four pesticides, with the values for the inhibition constant (Ki) varying from 0.12 to 20 µmol L-1, with the following order of inhibition from the highest to the lowest Ki value: deltamethrin > cypermethrin > allethrin > fenpropathrin. They presented a competitive and non-competitive inhibition mechanism depending on the compound. The IC50 values (concentration that inhibits 50% of the enzyme activity) showed the insecticide deltamethrin with greater inhibitory capacity, since it reduced the activity of the GST enzyme by half, even though it was in a lower concentration in relation to the other analyzed compounds. The in silico evaluation indicated the possibility of most of the insecticides contaminating the surface waters (rivers and lakes) adhered to the suspended sediment, but did not show any potential for contamination of groundwater through leaching. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422022000200138 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422022000200138 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
10.21577/0100-4042.20170804 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Química Nova v.45 n.2 2022 reponame:Química Nova (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Química Nova (Online) |
collection |
Química Nova (Online) |
repository.name.fl_str_mv |
Química Nova (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
quimicanova@sbq.org.br |
_version_ |
1750318121562931200 |