Immobilization of laccase on hybrid layered double hydroxide

Detalhes bibliográficos
Autor(a) principal: Camacho Córdova,David Isidoro
Data de Publicação: 2009
Outros Autores: Morales Borges,Rogelio, Arizaga,Gregorio Guadalupe Carbajal, Wypych,Fernando, Krieger,Nádia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000600025
Resumo: Crystals of Mg/Al layered double hydroxide were synthesized by alkaline precipitation and treated in an aqueous solution of glutamic acid. The glutamate ions were not intercalated into the interlayer space, but were detected in the material by Fourier transform infrared spectroscopy, suggesting that only the external surfaces of crystals were modified with glutamate ions. The resulting hybrid material was tested as a support for immobilization of the enzyme laccase (Myceliophthora thermophila). The immobilized enzyme preparation was characterized by electronic paramagnetic resonance spectroscopy and by assays of catalytic activity. The activity of the immobilized laccase was 97% of the activity in the free enzyme. Layered double hydroxide is a suitable support for use in remediation of soil studies.
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spelling Immobilization of laccase on hybrid layered double hydroxidelaccaselayered double hydroxideimmobilizationCrystals of Mg/Al layered double hydroxide were synthesized by alkaline precipitation and treated in an aqueous solution of glutamic acid. The glutamate ions were not intercalated into the interlayer space, but were detected in the material by Fourier transform infrared spectroscopy, suggesting that only the external surfaces of crystals were modified with glutamate ions. The resulting hybrid material was tested as a support for immobilization of the enzyme laccase (Myceliophthora thermophila). The immobilized enzyme preparation was characterized by electronic paramagnetic resonance spectroscopy and by assays of catalytic activity. The activity of the immobilized laccase was 97% of the activity in the free enzyme. Layered double hydroxide is a suitable support for use in remediation of soil studies.Sociedade Brasileira de Química2009-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000600025Química Nova v.32 n.6 2009reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422009000600025info:eu-repo/semantics/openAccessCamacho Córdova,David IsidoroMorales Borges,RogelioArizaga,Gregorio Guadalupe CarbajalWypych,FernandoKrieger,Nádiaeng2009-09-22T00:00:00Zoai:scielo:S0100-40422009000600025Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2009-09-22T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Immobilization of laccase on hybrid layered double hydroxide
title Immobilization of laccase on hybrid layered double hydroxide
spellingShingle Immobilization of laccase on hybrid layered double hydroxide
Camacho Córdova,David Isidoro
laccase
layered double hydroxide
immobilization
title_short Immobilization of laccase on hybrid layered double hydroxide
title_full Immobilization of laccase on hybrid layered double hydroxide
title_fullStr Immobilization of laccase on hybrid layered double hydroxide
title_full_unstemmed Immobilization of laccase on hybrid layered double hydroxide
title_sort Immobilization of laccase on hybrid layered double hydroxide
author Camacho Córdova,David Isidoro
author_facet Camacho Córdova,David Isidoro
Morales Borges,Rogelio
Arizaga,Gregorio Guadalupe Carbajal
Wypych,Fernando
Krieger,Nádia
author_role author
author2 Morales Borges,Rogelio
Arizaga,Gregorio Guadalupe Carbajal
Wypych,Fernando
Krieger,Nádia
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Camacho Córdova,David Isidoro
Morales Borges,Rogelio
Arizaga,Gregorio Guadalupe Carbajal
Wypych,Fernando
Krieger,Nádia
dc.subject.por.fl_str_mv laccase
layered double hydroxide
immobilization
topic laccase
layered double hydroxide
immobilization
description Crystals of Mg/Al layered double hydroxide were synthesized by alkaline precipitation and treated in an aqueous solution of glutamic acid. The glutamate ions were not intercalated into the interlayer space, but were detected in the material by Fourier transform infrared spectroscopy, suggesting that only the external surfaces of crystals were modified with glutamate ions. The resulting hybrid material was tested as a support for immobilization of the enzyme laccase (Myceliophthora thermophila). The immobilized enzyme preparation was characterized by electronic paramagnetic resonance spectroscopy and by assays of catalytic activity. The activity of the immobilized laccase was 97% of the activity in the free enzyme. Layered double hydroxide is a suitable support for use in remediation of soil studies.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000600025
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000600025
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-40422009000600025
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.32 n.6 2009
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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