Derivative spectrophotometric method for determination of acyclovir in polymeric nanoparticles

• Autor(a) principal: Tavares,Guilherme Diniz
• Data de Publicação: 2012
• Outros Autores: Ishikawa,Gisele Miki, Monteiro,Talita Ferreira, Zanolini,Carolina, Kedor-Hackmann,Érika Rosa Maria, Bou-Chacra,Nádia Araci, Consiglieri,Vladi Olga
• Tipo de documento: Relatório
• Idioma: eng
• Título da fonte: Química Nova (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012000100035
• topic: acyclovir; polymeric nanoparticles; derivative spectrophotometry

A derivative spectrophotometric method was validated for quantification of acyclovir in poly (n-butylcyanoacrylate) (PBCA) nanoparticles. Specificity, linearity, precision, accuracy, recovery, detection (LOD) and quantification (LOQ) limits were established for method validation. First-derivative at 295.2 nm eliminated interferences from nanoparticle ingredients and presented linearity for acyclovir concentrations ranging from 1.25 to 40.0 µg/mL (r = 0.9999). Precision and accuracy data demonstrated good reproducibility. Recovery ranged from 99.3 to 101.2. LOD was 0.08 µg/mL and LOQ, 0.25 µg/mL. Thus, the proposed method proved to be easy, low cost, and accurate, and therefore, an useful alternative to quantify acyclovir in nanoparticles.