HOTSPOT STABILIZATION OF GOLD NANOPARTICLES FOR APPLICATION OF QUANTITATIVE SERS IN BIOANALYTICAL SYSTEMS

Detalhes bibliográficos
Autor(a) principal: Mamián-López,Mónica B.
Data de Publicação: 2019
Outros Autores: Temperini,Marcia L. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422019004901084
Resumo: A complete procedure including synthesizing gold nanoparticles and further hotspots stabilization is presented. Colloidal gold synthesis followed a citrate reducing reaction, then, an ideal condition for SERS enhancement with quantitative purposes was reached by searching equilibrium between salt- and analyte-induced aggregation. Under this condition, a mild aggregation is produced, along with "free sites" available on the metal surface where analyte could easily interact. As a consequence, suitable linear concentration intervals and high quality SERS spectra were obtained allowing building multivariate quantitative models. For adenine as probe molecule, LOD values were around 10-8 mol L-1, even at low laser power and short exposure time during spectral acquisition. The hotspot stabilization procedure, as shown here, allows dealing with samples or highly complex analytical matrices that can easily cause undesirable agglomerate formation like those from biological origin.
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spelling HOTSPOT STABILIZATION OF GOLD NANOPARTICLES FOR APPLICATION OF QUANTITATIVE SERS IN BIOANALYTICAL SYSTEMSSERSgold nanoparticlesbioanalyticaladeninequantitative SERSA complete procedure including synthesizing gold nanoparticles and further hotspots stabilization is presented. Colloidal gold synthesis followed a citrate reducing reaction, then, an ideal condition for SERS enhancement with quantitative purposes was reached by searching equilibrium between salt- and analyte-induced aggregation. Under this condition, a mild aggregation is produced, along with "free sites" available on the metal surface where analyte could easily interact. As a consequence, suitable linear concentration intervals and high quality SERS spectra were obtained allowing building multivariate quantitative models. For adenine as probe molecule, LOD values were around 10-8 mol L-1, even at low laser power and short exposure time during spectral acquisition. The hotspot stabilization procedure, as shown here, allows dealing with samples or highly complex analytical matrices that can easily cause undesirable agglomerate formation like those from biological origin.Sociedade Brasileira de Química2019-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422019004901084Química Nova v.42 n.9 2019reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0100-4042.20170424info:eu-repo/semantics/openAccessMamián-López,Mónica B.Temperini,Marcia L. A.eng2019-12-09T00:00:00Zoai:scielo:S0100-40422019004901084Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2019-12-09T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv HOTSPOT STABILIZATION OF GOLD NANOPARTICLES FOR APPLICATION OF QUANTITATIVE SERS IN BIOANALYTICAL SYSTEMS
title HOTSPOT STABILIZATION OF GOLD NANOPARTICLES FOR APPLICATION OF QUANTITATIVE SERS IN BIOANALYTICAL SYSTEMS
spellingShingle HOTSPOT STABILIZATION OF GOLD NANOPARTICLES FOR APPLICATION OF QUANTITATIVE SERS IN BIOANALYTICAL SYSTEMS
Mamián-López,Mónica B.
SERS
gold nanoparticles
bioanalytical
adenine
quantitative SERS
title_short HOTSPOT STABILIZATION OF GOLD NANOPARTICLES FOR APPLICATION OF QUANTITATIVE SERS IN BIOANALYTICAL SYSTEMS
title_full HOTSPOT STABILIZATION OF GOLD NANOPARTICLES FOR APPLICATION OF QUANTITATIVE SERS IN BIOANALYTICAL SYSTEMS
title_fullStr HOTSPOT STABILIZATION OF GOLD NANOPARTICLES FOR APPLICATION OF QUANTITATIVE SERS IN BIOANALYTICAL SYSTEMS
title_full_unstemmed HOTSPOT STABILIZATION OF GOLD NANOPARTICLES FOR APPLICATION OF QUANTITATIVE SERS IN BIOANALYTICAL SYSTEMS
title_sort HOTSPOT STABILIZATION OF GOLD NANOPARTICLES FOR APPLICATION OF QUANTITATIVE SERS IN BIOANALYTICAL SYSTEMS
author Mamián-López,Mónica B.
author_facet Mamián-López,Mónica B.
Temperini,Marcia L. A.
author_role author
author2 Temperini,Marcia L. A.
author2_role author
dc.contributor.author.fl_str_mv Mamián-López,Mónica B.
Temperini,Marcia L. A.
dc.subject.por.fl_str_mv SERS
gold nanoparticles
bioanalytical
adenine
quantitative SERS
topic SERS
gold nanoparticles
bioanalytical
adenine
quantitative SERS
description A complete procedure including synthesizing gold nanoparticles and further hotspots stabilization is presented. Colloidal gold synthesis followed a citrate reducing reaction, then, an ideal condition for SERS enhancement with quantitative purposes was reached by searching equilibrium between salt- and analyte-induced aggregation. Under this condition, a mild aggregation is produced, along with "free sites" available on the metal surface where analyte could easily interact. As a consequence, suitable linear concentration intervals and high quality SERS spectra were obtained allowing building multivariate quantitative models. For adenine as probe molecule, LOD values were around 10-8 mol L-1, even at low laser power and short exposure time during spectral acquisition. The hotspot stabilization procedure, as shown here, allows dealing with samples or highly complex analytical matrices that can easily cause undesirable agglomerate formation like those from biological origin.
publishDate 2019
dc.date.none.fl_str_mv 2019-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422019004901084
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422019004901084
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.21577/0100-4042.20170424
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.42 n.9 2019
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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