BIOPRODUCTION AND CHARACTERIZATION OF SODIUM, POTASSIUM, AND CALCIUM LACTOBIONATES
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Química Nova (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422017000901003 |
Resumo: | Lactobionic acid and its salts are substances that have several applications in the pharmaceutical area. These products were obtained by bioconversion through the enzymatic complex glucose-fructose-oxidoreductase (GFOR)/gluconolactonase (GL) present in calcium alginate immobilized cells of Zymomonas mobilis. In the reactions catalyzed by this enzyme system, the medium pH must be controlled at slightly acid values. For this purpose, NaOH, KOH, or Ca(OH)2 were used, and as a result, the respective salts were formed. The kinetic study on the formation of sodium, potassium and calcium lactobionates was followed by the steps of purification and characterization aiming the potential use of these compounds in the pharmaceutical area. In the assays for the bioproduction of sodium, potassium or calcium lactobionates, yields of 74, 77 and 84% were obtained, respectively. In the salts purification step, purity levels of approximately 95% were achieved. The structural identities of the lactobionate salts were determined by high resolution mass spectrometry, in addition to the 13C and 1H NMR analysis. The characterization demonstrates the selectivity of the enzymatic reaction of GFOR/GL of Z. mobilis, in the production of lactobionic acid and its salts. |
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BIOPRODUCTION AND CHARACTERIZATION OF SODIUM, POTASSIUM, AND CALCIUM LACTOBIONATESZymomonas mobilislactobionatelactobionic acidcharacterizationmass spectrometry13C and 1H NMR analysisLactobionic acid and its salts are substances that have several applications in the pharmaceutical area. These products were obtained by bioconversion through the enzymatic complex glucose-fructose-oxidoreductase (GFOR)/gluconolactonase (GL) present in calcium alginate immobilized cells of Zymomonas mobilis. In the reactions catalyzed by this enzyme system, the medium pH must be controlled at slightly acid values. For this purpose, NaOH, KOH, or Ca(OH)2 were used, and as a result, the respective salts were formed. The kinetic study on the formation of sodium, potassium and calcium lactobionates was followed by the steps of purification and characterization aiming the potential use of these compounds in the pharmaceutical area. In the assays for the bioproduction of sodium, potassium or calcium lactobionates, yields of 74, 77 and 84% were obtained, respectively. In the salts purification step, purity levels of approximately 95% were achieved. The structural identities of the lactobionate salts were determined by high resolution mass spectrometry, in addition to the 13C and 1H NMR analysis. The characterization demonstrates the selectivity of the enzymatic reaction of GFOR/GL of Z. mobilis, in the production of lactobionic acid and its salts.Sociedade Brasileira de Química2017-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422017000901003Química Nova v.40 n.9 2017reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0100-4042.20170108info:eu-repo/semantics/openAccessDelagustin,Maria G.Gonçalves,EduardaCarra,SabrinaBarcellos,ThiagoBassani,Valquíria L.Silveira,Mauricio M.Malvessi,Eloaneeng2017-11-21T00:00:00Zoai:scielo:S0100-40422017000901003Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2017-11-21T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
BIOPRODUCTION AND CHARACTERIZATION OF SODIUM, POTASSIUM, AND CALCIUM LACTOBIONATES |
title |
BIOPRODUCTION AND CHARACTERIZATION OF SODIUM, POTASSIUM, AND CALCIUM LACTOBIONATES |
spellingShingle |
BIOPRODUCTION AND CHARACTERIZATION OF SODIUM, POTASSIUM, AND CALCIUM LACTOBIONATES Delagustin,Maria G. Zymomonas mobilis lactobionate lactobionic acid characterization mass spectrometry 13C and 1H NMR analysis |
title_short |
BIOPRODUCTION AND CHARACTERIZATION OF SODIUM, POTASSIUM, AND CALCIUM LACTOBIONATES |
title_full |
BIOPRODUCTION AND CHARACTERIZATION OF SODIUM, POTASSIUM, AND CALCIUM LACTOBIONATES |
title_fullStr |
BIOPRODUCTION AND CHARACTERIZATION OF SODIUM, POTASSIUM, AND CALCIUM LACTOBIONATES |
title_full_unstemmed |
BIOPRODUCTION AND CHARACTERIZATION OF SODIUM, POTASSIUM, AND CALCIUM LACTOBIONATES |
title_sort |
BIOPRODUCTION AND CHARACTERIZATION OF SODIUM, POTASSIUM, AND CALCIUM LACTOBIONATES |
author |
Delagustin,Maria G. |
author_facet |
Delagustin,Maria G. Gonçalves,Eduarda Carra,Sabrina Barcellos,Thiago Bassani,Valquíria L. Silveira,Mauricio M. Malvessi,Eloane |
author_role |
author |
author2 |
Gonçalves,Eduarda Carra,Sabrina Barcellos,Thiago Bassani,Valquíria L. Silveira,Mauricio M. Malvessi,Eloane |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Delagustin,Maria G. Gonçalves,Eduarda Carra,Sabrina Barcellos,Thiago Bassani,Valquíria L. Silveira,Mauricio M. Malvessi,Eloane |
dc.subject.por.fl_str_mv |
Zymomonas mobilis lactobionate lactobionic acid characterization mass spectrometry 13C and 1H NMR analysis |
topic |
Zymomonas mobilis lactobionate lactobionic acid characterization mass spectrometry 13C and 1H NMR analysis |
description |
Lactobionic acid and its salts are substances that have several applications in the pharmaceutical area. These products were obtained by bioconversion through the enzymatic complex glucose-fructose-oxidoreductase (GFOR)/gluconolactonase (GL) present in calcium alginate immobilized cells of Zymomonas mobilis. In the reactions catalyzed by this enzyme system, the medium pH must be controlled at slightly acid values. For this purpose, NaOH, KOH, or Ca(OH)2 were used, and as a result, the respective salts were formed. The kinetic study on the formation of sodium, potassium and calcium lactobionates was followed by the steps of purification and characterization aiming the potential use of these compounds in the pharmaceutical area. In the assays for the bioproduction of sodium, potassium or calcium lactobionates, yields of 74, 77 and 84% were obtained, respectively. In the salts purification step, purity levels of approximately 95% were achieved. The structural identities of the lactobionate salts were determined by high resolution mass spectrometry, in addition to the 13C and 1H NMR analysis. The characterization demonstrates the selectivity of the enzymatic reaction of GFOR/GL of Z. mobilis, in the production of lactobionic acid and its salts. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422017000901003 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422017000901003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.21577/0100-4042.20170108 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Química Nova v.40 n.9 2017 reponame:Química Nova (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Química Nova (Online) |
collection |
Química Nova (Online) |
repository.name.fl_str_mv |
Química Nova (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
quimicanova@sbq.org.br |
_version_ |
1750318118433980416 |