A fluorescence-based assay for octreotide in kinetic release from depot formulations

Detalhes bibliográficos
Autor(a) principal: Guerreiro,Luiz Henrique
Data de Publicação: 2012
Outros Autores: Girad-Dias,Wendell, Miranda,Kildare R. de, Lima,Luís Mauricio T. R.
Tipo de documento: Relatório
Idioma: eng
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012000500029
Resumo: Here we report the validation of a derivatization method that makes use of fluorescamine as a selective reactant for the quantitative analysis of peptide and protein drugs in the dissolution profile from depot formulations. Typical current methods require separation of the nano/microparticles and time-consuming chromatographic runs. In this study we report a method which can be conducted without the need for complete physical separation of the particles or removal of the unreacted probe. This method was used here for the analysis of the release profile of octreotide in a depot formulation, with results in excellent agreement with reported chromatographic assays.
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spelling A fluorescence-based assay for octreotide in kinetic release from depot formulationsoctreotidefluorescaminecontrolled releaseHere we report the validation of a derivatization method that makes use of fluorescamine as a selective reactant for the quantitative analysis of peptide and protein drugs in the dissolution profile from depot formulations. Typical current methods require separation of the nano/microparticles and time-consuming chromatographic runs. In this study we report a method which can be conducted without the need for complete physical separation of the particles or removal of the unreacted probe. This method was used here for the analysis of the release profile of octreotide in a depot formulation, with results in excellent agreement with reported chromatographic assays.Sociedade Brasileira de Química2012-01-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012000500029Química Nova v.35 n.5 2012reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422012000500029info:eu-repo/semantics/openAccessGuerreiro,Luiz HenriqueGirad-Dias,WendellMiranda,Kildare R. deLima,Luís Mauricio T. R.eng2012-07-02T00:00:00Zoai:scielo:S0100-40422012000500029Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2012-07-02T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv A fluorescence-based assay for octreotide in kinetic release from depot formulations
title A fluorescence-based assay for octreotide in kinetic release from depot formulations
spellingShingle A fluorescence-based assay for octreotide in kinetic release from depot formulations
Guerreiro,Luiz Henrique
octreotide
fluorescamine
controlled release
title_short A fluorescence-based assay for octreotide in kinetic release from depot formulations
title_full A fluorescence-based assay for octreotide in kinetic release from depot formulations
title_fullStr A fluorescence-based assay for octreotide in kinetic release from depot formulations
title_full_unstemmed A fluorescence-based assay for octreotide in kinetic release from depot formulations
title_sort A fluorescence-based assay for octreotide in kinetic release from depot formulations
author Guerreiro,Luiz Henrique
author_facet Guerreiro,Luiz Henrique
Girad-Dias,Wendell
Miranda,Kildare R. de
Lima,Luís Mauricio T. R.
author_role author
author2 Girad-Dias,Wendell
Miranda,Kildare R. de
Lima,Luís Mauricio T. R.
author2_role author
author
author
dc.contributor.author.fl_str_mv Guerreiro,Luiz Henrique
Girad-Dias,Wendell
Miranda,Kildare R. de
Lima,Luís Mauricio T. R.
dc.subject.por.fl_str_mv octreotide
fluorescamine
controlled release
topic octreotide
fluorescamine
controlled release
description Here we report the validation of a derivatization method that makes use of fluorescamine as a selective reactant for the quantitative analysis of peptide and protein drugs in the dissolution profile from depot formulations. Typical current methods require separation of the nano/microparticles and time-consuming chromatographic runs. In this study we report a method which can be conducted without the need for complete physical separation of the particles or removal of the unreacted probe. This method was used here for the analysis of the release profile of octreotide in a depot formulation, with results in excellent agreement with reported chromatographic assays.
publishDate 2012
dc.date.none.fl_str_mv 2012-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/report
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format report
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012000500029
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012000500029
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-40422012000500029
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.35 n.5 2012
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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