High performance thin layer chromatographic determination of nifedipine in human serum after liquid-liquid extraction
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Química Nova (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012000200032 |
Resumo: | A method using HPTLC for quantitation of nifedipine in serum was developed and validated. It includes a liquid-liquid extraction, and carbamazepine as internal standard. Chloroform: ethyl acetate: cyclohexane (19:2:2, v/v/v) was the mobile phase. The method showed good relationship (r = 0.996) (2.00 to 25.00 ng/band, corresponding to 0.02 and 0.25 ng/µL in serum). The % RSD of intra-assay and inter-assay, were between 0.57 and 3.56 and 1.16 to 3.60, respectively. LOD and LOQ were 0.72 and 0.86 ng/band, respectively. The recovery values were between 93 and 102%. Rf for nifedipine and carbamazepine were 0.31 and 0.10, respectively. |
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High performance thin layer chromatographic determination of nifedipine in human serum after liquid-liquid extractionnifedipinehigh performance thin layer chromatographyquantitative determinationA method using HPTLC for quantitation of nifedipine in serum was developed and validated. It includes a liquid-liquid extraction, and carbamazepine as internal standard. Chloroform: ethyl acetate: cyclohexane (19:2:2, v/v/v) was the mobile phase. The method showed good relationship (r = 0.996) (2.00 to 25.00 ng/band, corresponding to 0.02 and 0.25 ng/µL in serum). The % RSD of intra-assay and inter-assay, were between 0.57 and 3.56 and 1.16 to 3.60, respectively. LOD and LOQ were 0.72 and 0.86 ng/band, respectively. The recovery values were between 93 and 102%. Rf for nifedipine and carbamazepine were 0.31 and 0.10, respectively.Sociedade Brasileira de Química2012-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012000200032Química Nova v.35 n.2 2012reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422012000200032info:eu-repo/semantics/openAccessMennickent,SigridContreras,JonathanSchulz,BertaDiego,Marta deVega,Marioeng2012-04-03T00:00:00Zoai:scielo:S0100-40422012000200032Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2012-04-03T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
High performance thin layer chromatographic determination of nifedipine in human serum after liquid-liquid extraction |
title |
High performance thin layer chromatographic determination of nifedipine in human serum after liquid-liquid extraction |
spellingShingle |
High performance thin layer chromatographic determination of nifedipine in human serum after liquid-liquid extraction Mennickent,Sigrid nifedipine high performance thin layer chromatography quantitative determination |
title_short |
High performance thin layer chromatographic determination of nifedipine in human serum after liquid-liquid extraction |
title_full |
High performance thin layer chromatographic determination of nifedipine in human serum after liquid-liquid extraction |
title_fullStr |
High performance thin layer chromatographic determination of nifedipine in human serum after liquid-liquid extraction |
title_full_unstemmed |
High performance thin layer chromatographic determination of nifedipine in human serum after liquid-liquid extraction |
title_sort |
High performance thin layer chromatographic determination of nifedipine in human serum after liquid-liquid extraction |
author |
Mennickent,Sigrid |
author_facet |
Mennickent,Sigrid Contreras,Jonathan Schulz,Berta Diego,Marta de Vega,Mario |
author_role |
author |
author2 |
Contreras,Jonathan Schulz,Berta Diego,Marta de Vega,Mario |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Mennickent,Sigrid Contreras,Jonathan Schulz,Berta Diego,Marta de Vega,Mario |
dc.subject.por.fl_str_mv |
nifedipine high performance thin layer chromatography quantitative determination |
topic |
nifedipine high performance thin layer chromatography quantitative determination |
description |
A method using HPTLC for quantitation of nifedipine in serum was developed and validated. It includes a liquid-liquid extraction, and carbamazepine as internal standard. Chloroform: ethyl acetate: cyclohexane (19:2:2, v/v/v) was the mobile phase. The method showed good relationship (r = 0.996) (2.00 to 25.00 ng/band, corresponding to 0.02 and 0.25 ng/µL in serum). The % RSD of intra-assay and inter-assay, were between 0.57 and 3.56 and 1.16 to 3.60, respectively. LOD and LOQ were 0.72 and 0.86 ng/band, respectively. The recovery values were between 93 and 102%. Rf for nifedipine and carbamazepine were 0.31 and 0.10, respectively. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012000200032 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012000200032 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-40422012000200032 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Química Nova v.35 n.2 2012 reponame:Química Nova (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Química Nova (Online) |
collection |
Química Nova (Online) |
repository.name.fl_str_mv |
Química Nova (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
quimicanova@sbq.org.br |
_version_ |
1750318113106165760 |