Quantitation of genistein and genistin in soy dry extracts by UV-Visible spectrophotometric method

Detalhes bibliográficos
Autor(a) principal: César,Isabela da Costa
Data de Publicação: 2008
Outros Autores: Braga,Fernão Castro, Vianna-Soares,Cristina Duarte, Nunan,Elzíria de Aguiar, Pianetti,Gérson Antônio, Moreira-Campos,Ligia Maria
Tipo de documento: Artigo
Idioma: por
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422008000800003
Resumo: This paper describes the development and validation of an UV-Visible spectrophotometric method for quantitation of genistein and genistin in soy dry extracts, after reaction with aluminum chloride. The method showed to be linear (r²= 0.9999), precise (R.S.D. < 2%), accurate (recovery of 101.56%) and robust. Seven samples of soy dry extracts were analyzed by the spectrophotometric validated method and by RP-HPLC. Genistein concentrations determined by spectrophotometry (0.63% - 16.05%) were slightly higher than values obtained by HPLC analysis (0.40% - 12.79%); however, the results of both methods showed a strong correlation.
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spelling Quantitation of genistein and genistin in soy dry extracts by UV-Visible spectrophotometric methodisoflavonesspectrophotometryRP-HPLCThis paper describes the development and validation of an UV-Visible spectrophotometric method for quantitation of genistein and genistin in soy dry extracts, after reaction with aluminum chloride. The method showed to be linear (r²= 0.9999), precise (R.S.D. < 2%), accurate (recovery of 101.56%) and robust. Seven samples of soy dry extracts were analyzed by the spectrophotometric validated method and by RP-HPLC. Genistein concentrations determined by spectrophotometry (0.63% - 16.05%) were slightly higher than values obtained by HPLC analysis (0.40% - 12.79%); however, the results of both methods showed a strong correlation.Sociedade Brasileira de Química2008-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422008000800003Química Nova v.31 n.8 2008reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422008000800003info:eu-repo/semantics/openAccessCésar,Isabela da CostaBraga,Fernão CastroVianna-Soares,Cristina DuarteNunan,Elzíria de AguiarPianetti,Gérson AntônioMoreira-Campos,Ligia Mariapor2009-01-16T00:00:00Zoai:scielo:S0100-40422008000800003Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2009-01-16T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Quantitation of genistein and genistin in soy dry extracts by UV-Visible spectrophotometric method
title Quantitation of genistein and genistin in soy dry extracts by UV-Visible spectrophotometric method
spellingShingle Quantitation of genistein and genistin in soy dry extracts by UV-Visible spectrophotometric method
César,Isabela da Costa
isoflavones
spectrophotometry
RP-HPLC
title_short Quantitation of genistein and genistin in soy dry extracts by UV-Visible spectrophotometric method
title_full Quantitation of genistein and genistin in soy dry extracts by UV-Visible spectrophotometric method
title_fullStr Quantitation of genistein and genistin in soy dry extracts by UV-Visible spectrophotometric method
title_full_unstemmed Quantitation of genistein and genistin in soy dry extracts by UV-Visible spectrophotometric method
title_sort Quantitation of genistein and genistin in soy dry extracts by UV-Visible spectrophotometric method
author César,Isabela da Costa
author_facet César,Isabela da Costa
Braga,Fernão Castro
Vianna-Soares,Cristina Duarte
Nunan,Elzíria de Aguiar
Pianetti,Gérson Antônio
Moreira-Campos,Ligia Maria
author_role author
author2 Braga,Fernão Castro
Vianna-Soares,Cristina Duarte
Nunan,Elzíria de Aguiar
Pianetti,Gérson Antônio
Moreira-Campos,Ligia Maria
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv César,Isabela da Costa
Braga,Fernão Castro
Vianna-Soares,Cristina Duarte
Nunan,Elzíria de Aguiar
Pianetti,Gérson Antônio
Moreira-Campos,Ligia Maria
dc.subject.por.fl_str_mv isoflavones
spectrophotometry
RP-HPLC
topic isoflavones
spectrophotometry
RP-HPLC
description This paper describes the development and validation of an UV-Visible spectrophotometric method for quantitation of genistein and genistin in soy dry extracts, after reaction with aluminum chloride. The method showed to be linear (r²= 0.9999), precise (R.S.D. < 2%), accurate (recovery of 101.56%) and robust. Seven samples of soy dry extracts were analyzed by the spectrophotometric validated method and by RP-HPLC. Genistein concentrations determined by spectrophotometry (0.63% - 16.05%) were slightly higher than values obtained by HPLC analysis (0.40% - 12.79%); however, the results of both methods showed a strong correlation.
publishDate 2008
dc.date.none.fl_str_mv 2008-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422008000800003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422008000800003
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv 10.1590/S0100-40422008000800003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.31 n.8 2008
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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