SÍNTESE ENZIMÁTICA DO CAPRILATO DE ISOPENTILA UTILIZANDO ÓLEO FÚSEL COMO MATÉRIA-PRIMA

Detalhes bibliográficos
Autor(a) principal: Bôas,Renata N. Vilas
Data de Publicação: 2017
Outros Autores: Biaggio,Francisco C., Giordani,Domingos S., Castro,Heizir F. de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422017000500541
Resumo: Four commercial lipase preparations immobilized by covalent attachment on epoxy-polysiloxane-β-cyclodextrin were tested as potential biocatalysts to mediate the esterification reaction of isopentanol with caprylic acid in solvent-free medium. Among them, the immobilized lipase from Rhizopus oryzae was the most active biocatalyst, attained ester conversion higher than 80% in 24 h, being selected for subsequent tests using fusel oil as source of isopentanol. An experimental design was performed and the analysis of the results revealed that the formation of the isopentyl caprylate was strongly influenced by the variable molar ratio at 95% of confidence level. The proposed mathematical model allowed predicting that the excess of caprylic acid (molar ratio fusel oil to acid of 1:1.5) and reaction temperature of 45 ºC favor the reaction to attain high ester conversion. Sensory evaluation of the flavors profile in relation to all the components involved in the reaction (caprylic acid, fusel oil and esters) showed that the formed esters had distinct aromas from the starting materials, confirming that the changes in flavors occurred before and after esterification reactions.
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spelling SÍNTESE ENZIMÁTICA DO CAPRILATO DE ISOPENTILA UTILIZANDO ÓLEO FÚSEL COMO MATÉRIA-PRIMAisopentyl caprylateimmobilized lipasefusel oiloptimizationesterificationFour commercial lipase preparations immobilized by covalent attachment on epoxy-polysiloxane-β-cyclodextrin were tested as potential biocatalysts to mediate the esterification reaction of isopentanol with caprylic acid in solvent-free medium. Among them, the immobilized lipase from Rhizopus oryzae was the most active biocatalyst, attained ester conversion higher than 80% in 24 h, being selected for subsequent tests using fusel oil as source of isopentanol. An experimental design was performed and the analysis of the results revealed that the formation of the isopentyl caprylate was strongly influenced by the variable molar ratio at 95% of confidence level. The proposed mathematical model allowed predicting that the excess of caprylic acid (molar ratio fusel oil to acid of 1:1.5) and reaction temperature of 45 ºC favor the reaction to attain high ester conversion. Sensory evaluation of the flavors profile in relation to all the components involved in the reaction (caprylic acid, fusel oil and esters) showed that the formed esters had distinct aromas from the starting materials, confirming that the changes in flavors occurred before and after esterification reactions.Sociedade Brasileira de Química2017-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422017000500541Química Nova v.40 n.5 2017reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0100-4042.20170032info:eu-repo/semantics/openAccessBôas,Renata N. VilasBiaggio,Francisco C.Giordani,Domingos S.Castro,Heizir F. depor2017-07-04T00:00:00Zoai:scielo:S0100-40422017000500541Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2017-07-04T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv SÍNTESE ENZIMÁTICA DO CAPRILATO DE ISOPENTILA UTILIZANDO ÓLEO FÚSEL COMO MATÉRIA-PRIMA
title SÍNTESE ENZIMÁTICA DO CAPRILATO DE ISOPENTILA UTILIZANDO ÓLEO FÚSEL COMO MATÉRIA-PRIMA
spellingShingle SÍNTESE ENZIMÁTICA DO CAPRILATO DE ISOPENTILA UTILIZANDO ÓLEO FÚSEL COMO MATÉRIA-PRIMA
Bôas,Renata N. Vilas
isopentyl caprylate
immobilized lipase
fusel oil
optimization
esterification
title_short SÍNTESE ENZIMÁTICA DO CAPRILATO DE ISOPENTILA UTILIZANDO ÓLEO FÚSEL COMO MATÉRIA-PRIMA
title_full SÍNTESE ENZIMÁTICA DO CAPRILATO DE ISOPENTILA UTILIZANDO ÓLEO FÚSEL COMO MATÉRIA-PRIMA
title_fullStr SÍNTESE ENZIMÁTICA DO CAPRILATO DE ISOPENTILA UTILIZANDO ÓLEO FÚSEL COMO MATÉRIA-PRIMA
title_full_unstemmed SÍNTESE ENZIMÁTICA DO CAPRILATO DE ISOPENTILA UTILIZANDO ÓLEO FÚSEL COMO MATÉRIA-PRIMA
title_sort SÍNTESE ENZIMÁTICA DO CAPRILATO DE ISOPENTILA UTILIZANDO ÓLEO FÚSEL COMO MATÉRIA-PRIMA
author Bôas,Renata N. Vilas
author_facet Bôas,Renata N. Vilas
Biaggio,Francisco C.
Giordani,Domingos S.
Castro,Heizir F. de
author_role author
author2 Biaggio,Francisco C.
Giordani,Domingos S.
Castro,Heizir F. de
author2_role author
author
author
dc.contributor.author.fl_str_mv Bôas,Renata N. Vilas
Biaggio,Francisco C.
Giordani,Domingos S.
Castro,Heizir F. de
dc.subject.por.fl_str_mv isopentyl caprylate
immobilized lipase
fusel oil
optimization
esterification
topic isopentyl caprylate
immobilized lipase
fusel oil
optimization
esterification
description Four commercial lipase preparations immobilized by covalent attachment on epoxy-polysiloxane-β-cyclodextrin were tested as potential biocatalysts to mediate the esterification reaction of isopentanol with caprylic acid in solvent-free medium. Among them, the immobilized lipase from Rhizopus oryzae was the most active biocatalyst, attained ester conversion higher than 80% in 24 h, being selected for subsequent tests using fusel oil as source of isopentanol. An experimental design was performed and the analysis of the results revealed that the formation of the isopentyl caprylate was strongly influenced by the variable molar ratio at 95% of confidence level. The proposed mathematical model allowed predicting that the excess of caprylic acid (molar ratio fusel oil to acid of 1:1.5) and reaction temperature of 45 ºC favor the reaction to attain high ester conversion. Sensory evaluation of the flavors profile in relation to all the components involved in the reaction (caprylic acid, fusel oil and esters) showed that the formed esters had distinct aromas from the starting materials, confirming that the changes in flavors occurred before and after esterification reactions.
publishDate 2017
dc.date.none.fl_str_mv 2017-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422017000500541
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422017000500541
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv 10.21577/0100-4042.20170032
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.40 n.5 2017
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
_version_ 1750318118330171392

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