A validated HPLC analytical method for the analysis of solasonine and solamargine in in vitro skin penetration studies

Detalhes bibliográficos
Autor(a) principal: Tiossi,Renata F. J.
Data de Publicação: 2012
Outros Autores: Da Costa,Juliana C., Miranda,Mariza A., Praça,Fabíola S. G., Bentley,Maria Vitória L. B., Bastos,Jairo K., McChesney,James D.
Tipo de documento: Relatório
Idioma: eng
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012001100038
Resumo: To assess topical delivery studies of glycoalkaloids, an analytical method by HPLC-UV was developed and validated for the determination of solasonine (SN) and solamargine (SM) in different skin layers, as well as in a topical formulation. The method was linear within the ranges 0.86 to 990.00 µg/mL for SN and 1.74 to 1000.00 µg/mL for SM (r = 0.9996). Moreover, the recoveries for both glycoalkaloids were higher than 88.94 and 93.23% from skin samples and topical formulation, respectively. The method developed is reliable and suitable for topical delivery skin studies and for determining the content of SN and SM in topical formulations.
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spelling A validated HPLC analytical method for the analysis of solasonine and solamargine in in vitro skin penetration studiessolasoninesolamarginevalidationTo assess topical delivery studies of glycoalkaloids, an analytical method by HPLC-UV was developed and validated for the determination of solasonine (SN) and solamargine (SM) in different skin layers, as well as in a topical formulation. The method was linear within the ranges 0.86 to 990.00 µg/mL for SN and 1.74 to 1000.00 µg/mL for SM (r = 0.9996). Moreover, the recoveries for both glycoalkaloids were higher than 88.94 and 93.23% from skin samples and topical formulation, respectively. The method developed is reliable and suitable for topical delivery skin studies and for determining the content of SN and SM in topical formulations.Sociedade Brasileira de Química2012-01-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012001100038Química Nova v.35 n.11 2012reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422012001100038info:eu-repo/semantics/openAccessTiossi,Renata F. J.Da Costa,Juliana C.Miranda,Mariza A.Praça,Fabíola S. G.Bentley,Maria Vitória L. B.Bastos,Jairo K.McChesney,James D.eng2012-11-30T00:00:00Zoai:scielo:S0100-40422012001100038Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2012-11-30T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv A validated HPLC analytical method for the analysis of solasonine and solamargine in in vitro skin penetration studies
title A validated HPLC analytical method for the analysis of solasonine and solamargine in in vitro skin penetration studies
spellingShingle A validated HPLC analytical method for the analysis of solasonine and solamargine in in vitro skin penetration studies
Tiossi,Renata F. J.
solasonine
solamargine
validation
title_short A validated HPLC analytical method for the analysis of solasonine and solamargine in in vitro skin penetration studies
title_full A validated HPLC analytical method for the analysis of solasonine and solamargine in in vitro skin penetration studies
title_fullStr A validated HPLC analytical method for the analysis of solasonine and solamargine in in vitro skin penetration studies
title_full_unstemmed A validated HPLC analytical method for the analysis of solasonine and solamargine in in vitro skin penetration studies
title_sort A validated HPLC analytical method for the analysis of solasonine and solamargine in in vitro skin penetration studies
author Tiossi,Renata F. J.
author_facet Tiossi,Renata F. J.
Da Costa,Juliana C.
Miranda,Mariza A.
Praça,Fabíola S. G.
Bentley,Maria Vitória L. B.
Bastos,Jairo K.
McChesney,James D.
author_role author
author2 Da Costa,Juliana C.
Miranda,Mariza A.
Praça,Fabíola S. G.
Bentley,Maria Vitória L. B.
Bastos,Jairo K.
McChesney,James D.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Tiossi,Renata F. J.
Da Costa,Juliana C.
Miranda,Mariza A.
Praça,Fabíola S. G.
Bentley,Maria Vitória L. B.
Bastos,Jairo K.
McChesney,James D.
dc.subject.por.fl_str_mv solasonine
solamargine
validation
topic solasonine
solamargine
validation
description To assess topical delivery studies of glycoalkaloids, an analytical method by HPLC-UV was developed and validated for the determination of solasonine (SN) and solamargine (SM) in different skin layers, as well as in a topical formulation. The method was linear within the ranges 0.86 to 990.00 µg/mL for SN and 1.74 to 1000.00 µg/mL for SM (r = 0.9996). Moreover, the recoveries for both glycoalkaloids were higher than 88.94 and 93.23% from skin samples and topical formulation, respectively. The method developed is reliable and suitable for topical delivery skin studies and for determining the content of SN and SM in topical formulations.
publishDate 2012
dc.date.none.fl_str_mv 2012-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/report
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format report
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012001100038
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422012001100038
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-40422012001100038
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.35 n.11 2012
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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