Green tea in transdermal formulation: HPLC method for quality control and in vitro drug release assays

Detalhes bibliográficos
Autor(a) principal: Alves,Michele Campos
Data de Publicação: 2014
Outros Autores: Almeida,Priscila Aparecida de, Polonini,Hudson Caetano, Raposo,Nádia Rezende Barbosa, Ferreira,Anderson de Oliveira, Brandão,Marcos Antônio Fernandes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422014000400024
Resumo: RP-HPLC based analytical method for use in both quality control of green tea in a semisolid formulation and for in vitro drug release assays was developed and validated. The method was precise (CV < 5%), accurate (recovery between 98% and 102%), linear (R² &gt; 0.99), robust, and specific for the determination of epigallocatechin 3-gallate (EGCG), caffeine (CAF), and gallic acid (GA). In a diffusion cell chamber, the release rate of EGCG was 8896.01 µg cm-2. This data showed that EGCG will be able to exert its systemic activity when delivered though the transdermal formulation, due to its good flux rates with the synthetic membrane.
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spelling Green tea in transdermal formulation: HPLC method for quality control and in vitro drug release assaysCamellia sinensisquality controlin vitro drug releaseRP-HPLC based analytical method for use in both quality control of green tea in a semisolid formulation and for in vitro drug release assays was developed and validated. The method was precise (CV < 5%), accurate (recovery between 98% and 102%), linear (R² &gt; 0.99), robust, and specific for the determination of epigallocatechin 3-gallate (EGCG), caffeine (CAF), and gallic acid (GA). In a diffusion cell chamber, the release rate of EGCG was 8896.01 µg cm-2. This data showed that EGCG will be able to exert its systemic activity when delivered though the transdermal formulation, due to its good flux rates with the synthetic membrane.Sociedade Brasileira de Química2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422014000400024Química Nova v.37 n.4 2014reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0100-4042.20140117info:eu-repo/semantics/openAccessAlves,Michele CamposAlmeida,Priscila Aparecida dePolonini,Hudson CaetanoRaposo,Nádia Rezende BarbosaFerreira,Anderson de OliveiraBrandão,Marcos Antônio Fernandeseng2014-05-29T00:00:00Zoai:scielo:S0100-40422014000400024Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2014-05-29T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Green tea in transdermal formulation: HPLC method for quality control and in vitro drug release assays
title Green tea in transdermal formulation: HPLC method for quality control and in vitro drug release assays
spellingShingle Green tea in transdermal formulation: HPLC method for quality control and in vitro drug release assays
Alves,Michele Campos
Camellia sinensis
quality control
in vitro drug release
title_short Green tea in transdermal formulation: HPLC method for quality control and in vitro drug release assays
title_full Green tea in transdermal formulation: HPLC method for quality control and in vitro drug release assays
title_fullStr Green tea in transdermal formulation: HPLC method for quality control and in vitro drug release assays
title_full_unstemmed Green tea in transdermal formulation: HPLC method for quality control and in vitro drug release assays
title_sort Green tea in transdermal formulation: HPLC method for quality control and in vitro drug release assays
author Alves,Michele Campos
author_facet Alves,Michele Campos
Almeida,Priscila Aparecida de
Polonini,Hudson Caetano
Raposo,Nádia Rezende Barbosa
Ferreira,Anderson de Oliveira
Brandão,Marcos Antônio Fernandes
author_role author
author2 Almeida,Priscila Aparecida de
Polonini,Hudson Caetano
Raposo,Nádia Rezende Barbosa
Ferreira,Anderson de Oliveira
Brandão,Marcos Antônio Fernandes
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Alves,Michele Campos
Almeida,Priscila Aparecida de
Polonini,Hudson Caetano
Raposo,Nádia Rezende Barbosa
Ferreira,Anderson de Oliveira
Brandão,Marcos Antônio Fernandes
dc.subject.por.fl_str_mv Camellia sinensis
quality control
in vitro drug release
topic Camellia sinensis
quality control
in vitro drug release
description RP-HPLC based analytical method for use in both quality control of green tea in a semisolid formulation and for in vitro drug release assays was developed and validated. The method was precise (CV < 5%), accurate (recovery between 98% and 102%), linear (R² &gt; 0.99), robust, and specific for the determination of epigallocatechin 3-gallate (EGCG), caffeine (CAF), and gallic acid (GA). In a diffusion cell chamber, the release rate of EGCG was 8896.01 µg cm-2. This data showed that EGCG will be able to exert its systemic activity when delivered though the transdermal formulation, due to its good flux rates with the synthetic membrane.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422014000400024
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422014000400024
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.5935/0100-4042.20140117
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.37 n.4 2014
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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