Importance of the lipid peroxidation biomarkers and methodological aspects FOR malondialdehyde quantification

Detalhes bibliográficos
Autor(a) principal: Grotto,Denise
Data de Publicação: 2009
Outros Autores: Maria,Lucas Santa, Valentini,Juliana, Paniz,Clóvis, Schmitt,Gabriela, Garcia,Solange Cristina, Pomblum,Valdeci Juarez, Rocha,João Batista T., Farina,Marcelo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000100032
Resumo: Free radicals induce lipid peroxidation, playing an important role in pathological processes. The injury mediated by free radicals can be measured by conjugated dienes, malondialdehyde, 4-hydroxynonenal, and others. However, malondialdehyde has been pointed out as the main product to evaluate lipid peroxidation. Most assays determine malondialdehyde by its reaction with thiobarbituric acid, which can be measured by indirect (spectrometry) and direct methodologies (chromatography). Though there is some controversy among the methodologies, the selective HPLC-based assays provide a more reliable lipid peroxidation measure. This review describes significant aspects about MDA determination, its importance in pathologies and biological samples treatment.
id SBQ-3_f6bab7e0c0d936e03ce9140d75c24232
oai_identifier_str oai:scielo:S0100-40422009000100032
network_acronym_str SBQ-3
network_name_str Química Nova (Online)
repository_id_str
spelling Importance of the lipid peroxidation biomarkers and methodological aspects FOR malondialdehyde quantificationoxidative stressMDAmethodologiesFree radicals induce lipid peroxidation, playing an important role in pathological processes. The injury mediated by free radicals can be measured by conjugated dienes, malondialdehyde, 4-hydroxynonenal, and others. However, malondialdehyde has been pointed out as the main product to evaluate lipid peroxidation. Most assays determine malondialdehyde by its reaction with thiobarbituric acid, which can be measured by indirect (spectrometry) and direct methodologies (chromatography). Though there is some controversy among the methodologies, the selective HPLC-based assays provide a more reliable lipid peroxidation measure. This review describes significant aspects about MDA determination, its importance in pathologies and biological samples treatment.Sociedade Brasileira de Química2009-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000100032Química Nova v.32 n.1 2009reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422009000100032info:eu-repo/semantics/openAccessGrotto,DeniseMaria,Lucas SantaValentini,JulianaPaniz,ClóvisSchmitt,GabrielaGarcia,Solange CristinaPomblum,Valdeci JuarezRocha,João Batista T.Farina,Marceloeng2009-02-26T00:00:00Zoai:scielo:S0100-40422009000100032Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2009-02-26T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Importance of the lipid peroxidation biomarkers and methodological aspects FOR malondialdehyde quantification
title Importance of the lipid peroxidation biomarkers and methodological aspects FOR malondialdehyde quantification
spellingShingle Importance of the lipid peroxidation biomarkers and methodological aspects FOR malondialdehyde quantification
Grotto,Denise
oxidative stress
MDA
methodologies
title_short Importance of the lipid peroxidation biomarkers and methodological aspects FOR malondialdehyde quantification
title_full Importance of the lipid peroxidation biomarkers and methodological aspects FOR malondialdehyde quantification
title_fullStr Importance of the lipid peroxidation biomarkers and methodological aspects FOR malondialdehyde quantification
title_full_unstemmed Importance of the lipid peroxidation biomarkers and methodological aspects FOR malondialdehyde quantification
title_sort Importance of the lipid peroxidation biomarkers and methodological aspects FOR malondialdehyde quantification
author Grotto,Denise
author_facet Grotto,Denise
Maria,Lucas Santa
Valentini,Juliana
Paniz,Clóvis
Schmitt,Gabriela
Garcia,Solange Cristina
Pomblum,Valdeci Juarez
Rocha,João Batista T.
Farina,Marcelo
author_role author
author2 Maria,Lucas Santa
Valentini,Juliana
Paniz,Clóvis
Schmitt,Gabriela
Garcia,Solange Cristina
Pomblum,Valdeci Juarez
Rocha,João Batista T.
Farina,Marcelo
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Grotto,Denise
Maria,Lucas Santa
Valentini,Juliana
Paniz,Clóvis
Schmitt,Gabriela
Garcia,Solange Cristina
Pomblum,Valdeci Juarez
Rocha,João Batista T.
Farina,Marcelo
dc.subject.por.fl_str_mv oxidative stress
MDA
methodologies
topic oxidative stress
MDA
methodologies
description Free radicals induce lipid peroxidation, playing an important role in pathological processes. The injury mediated by free radicals can be measured by conjugated dienes, malondialdehyde, 4-hydroxynonenal, and others. However, malondialdehyde has been pointed out as the main product to evaluate lipid peroxidation. Most assays determine malondialdehyde by its reaction with thiobarbituric acid, which can be measured by indirect (spectrometry) and direct methodologies (chromatography). Though there is some controversy among the methodologies, the selective HPLC-based assays provide a more reliable lipid peroxidation measure. This review describes significant aspects about MDA determination, its importance in pathologies and biological samples treatment.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000100032
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000100032
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-40422009000100032
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.32 n.1 2009
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
_version_ 1750318108690612224

Registros relacionados