Method validation and stability study of quercetin in topical emulsions

Detalhes bibliográficos
Autor(a) principal: Casagrande,Rúbia
Data de Publicação: 2009
Outros Autores: Baracat,Marcela M., Georgetti,Sandra R., Verri Jr,Waldiceu A., Vicentini,Fabiana T. M. C., Rafael,Janice A., Jabor,José R., Fonseca,Maria J. V.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000700041
Resumo: This study validated a high performance liquid chromatography (HPLC) method for the quantitative evaluation of quercetin in topical emulsions. The method was linear within 0.05 - 200 μg/mL range with a correlation coefficient of 0.9997, and without interference in the quercetin peak. The detection and quantitation limits were 18 and 29 ng/mL, respectively. The intra- and inter-assay precisions presented R.S.D. values lower than 2%. An average of 93% and 94% of quercetin was recovered for non-ionic and anionic emulsions, respectively. The raw material and anionic emulsion, but not non-ionic emulsion, were stable in all storage conditions for one year. The method reported is a fast and reliable HPLC technique useful for quercetin determination in topical emulsions.
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spelling Method validation and stability study of quercetin in topical emulsionsquercetinHPLCvalidationThis study validated a high performance liquid chromatography (HPLC) method for the quantitative evaluation of quercetin in topical emulsions. The method was linear within 0.05 - 200 μg/mL range with a correlation coefficient of 0.9997, and without interference in the quercetin peak. The detection and quantitation limits were 18 and 29 ng/mL, respectively. The intra- and inter-assay precisions presented R.S.D. values lower than 2%. An average of 93% and 94% of quercetin was recovered for non-ionic and anionic emulsions, respectively. The raw material and anionic emulsion, but not non-ionic emulsion, were stable in all storage conditions for one year. The method reported is a fast and reliable HPLC technique useful for quercetin determination in topical emulsions.Sociedade Brasileira de Química2009-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000700041Química Nova v.32 n.7 2009reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422009000700041info:eu-repo/semantics/openAccessCasagrande,RúbiaBaracat,Marcela M.Georgetti,Sandra R.Verri Jr,Waldiceu A.Vicentini,Fabiana T. M. C.Rafael,Janice A.Jabor,José R.Fonseca,Maria J. V.eng2009-10-22T00:00:00Zoai:scielo:S0100-40422009000700041Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2009-10-22T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Method validation and stability study of quercetin in topical emulsions
title Method validation and stability study of quercetin in topical emulsions
spellingShingle Method validation and stability study of quercetin in topical emulsions
Casagrande,Rúbia
quercetin
HPLC
validation
title_short Method validation and stability study of quercetin in topical emulsions
title_full Method validation and stability study of quercetin in topical emulsions
title_fullStr Method validation and stability study of quercetin in topical emulsions
title_full_unstemmed Method validation and stability study of quercetin in topical emulsions
title_sort Method validation and stability study of quercetin in topical emulsions
author Casagrande,Rúbia
author_facet Casagrande,Rúbia
Baracat,Marcela M.
Georgetti,Sandra R.
Verri Jr,Waldiceu A.
Vicentini,Fabiana T. M. C.
Rafael,Janice A.
Jabor,José R.
Fonseca,Maria J. V.
author_role author
author2 Baracat,Marcela M.
Georgetti,Sandra R.
Verri Jr,Waldiceu A.
Vicentini,Fabiana T. M. C.
Rafael,Janice A.
Jabor,José R.
Fonseca,Maria J. V.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Casagrande,Rúbia
Baracat,Marcela M.
Georgetti,Sandra R.
Verri Jr,Waldiceu A.
Vicentini,Fabiana T. M. C.
Rafael,Janice A.
Jabor,José R.
Fonseca,Maria J. V.
dc.subject.por.fl_str_mv quercetin
HPLC
validation
topic quercetin
HPLC
validation
description This study validated a high performance liquid chromatography (HPLC) method for the quantitative evaluation of quercetin in topical emulsions. The method was linear within 0.05 - 200 μg/mL range with a correlation coefficient of 0.9997, and without interference in the quercetin peak. The detection and quantitation limits were 18 and 29 ng/mL, respectively. The intra- and inter-assay precisions presented R.S.D. values lower than 2%. An average of 93% and 94% of quercetin was recovered for non-ionic and anionic emulsions, respectively. The raw material and anionic emulsion, but not non-ionic emulsion, were stable in all storage conditions for one year. The method reported is a fast and reliable HPLC technique useful for quercetin determination in topical emulsions.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000700041
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000700041
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-40422009000700041
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.32 n.7 2009
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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