Molecular dereplication of volatile oils from Saururus cernuus L. and evaluation of anti-Trypanosoma cruzi activity
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Química Nova (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422022000200159 |
Resumo: | The present study reports the molecular dereplication and the evaluation of anti-Trypanosoma cruzi activity of volatile oils from inflorescences, leaves, branches, and roots of Saururus cernuus L. (Saururaceae). Chemically, the oils showed the predominance of sesquiterpenes in inflorescences (64.04%) and branches (63.82%), with β-sesquiphellandrene (25.50%) and (E)-caryophyllene (22.40%) corresponding to the main constituents of each oil. Furthermore, it was possible to detect safrole as the most predominant compound in the leaves oil (49.09%). On the other hand, the oil from roots was mainly composed by monoterpenes (84.60%), with limonene in higher concentration (38.41%), followed by α-pinene (20.19%), and camphene (14.71%). The oils from inflorescences and branches displayed higher antitrypanosomal potency with EC50 values of 7.1 and 8.8 μg mL-1, respectively, followed by the oils from the roots and inflorescences, with EC50 values of 17.3 and 30.4 μg mL-1, respectively. Additionally, branches and inflorescences oils displayed no toxicity in mammalian NCTC cells (CC50 > 200 μg mL-1). Using two PLS-DA methods, it was possible to suggest that the anti-T. cruzi activity of the tested oils could be associated with the presence of β-sesquiphellandrene, safrole, β-elemene, and α-zingiberene whereas threo-austrobailignan-5, β-sesquiphellandrene, α-humulene, germacrene D, and bicyclogermacrene play a role in the cytotoxicity against NCTC cells. |
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Molecular dereplication of volatile oils from Saururus cernuus L. and evaluation of anti-Trypanosoma cruzi activitySaururus cernuusvolatile oilsterpenessafrolTrypanosoma cruzi.The present study reports the molecular dereplication and the evaluation of anti-Trypanosoma cruzi activity of volatile oils from inflorescences, leaves, branches, and roots of Saururus cernuus L. (Saururaceae). Chemically, the oils showed the predominance of sesquiterpenes in inflorescences (64.04%) and branches (63.82%), with β-sesquiphellandrene (25.50%) and (E)-caryophyllene (22.40%) corresponding to the main constituents of each oil. Furthermore, it was possible to detect safrole as the most predominant compound in the leaves oil (49.09%). On the other hand, the oil from roots was mainly composed by monoterpenes (84.60%), with limonene in higher concentration (38.41%), followed by α-pinene (20.19%), and camphene (14.71%). The oils from inflorescences and branches displayed higher antitrypanosomal potency with EC50 values of 7.1 and 8.8 μg mL-1, respectively, followed by the oils from the roots and inflorescences, with EC50 values of 17.3 and 30.4 μg mL-1, respectively. Additionally, branches and inflorescences oils displayed no toxicity in mammalian NCTC cells (CC50 > 200 μg mL-1). Using two PLS-DA methods, it was possible to suggest that the anti-T. cruzi activity of the tested oils could be associated with the presence of β-sesquiphellandrene, safrole, β-elemene, and α-zingiberene whereas threo-austrobailignan-5, β-sesquiphellandrene, α-humulene, germacrene D, and bicyclogermacrene play a role in the cytotoxicity against NCTC cells.Sociedade Brasileira de Química2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422022000200159Química Nova v.45 n.2 2022reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0100-4042.20170811info:eu-repo/semantics/openAccessBrito,Juliana RCosta-Silva,Thais ALondero,Vinicius SBaldim,João LTempone,Andre GFerreira,Edgard ALago,João Henrique Geng2022-04-18T00:00:00Zoai:scielo:S0100-40422022000200159Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2022-04-18T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Molecular dereplication of volatile oils from Saururus cernuus L. and evaluation of anti-Trypanosoma cruzi activity |
title |
Molecular dereplication of volatile oils from Saururus cernuus L. and evaluation of anti-Trypanosoma cruzi activity |
spellingShingle |
Molecular dereplication of volatile oils from Saururus cernuus L. and evaluation of anti-Trypanosoma cruzi activity Brito,Juliana R Saururus cernuus volatile oils terpenes safrol Trypanosoma cruzi. |
title_short |
Molecular dereplication of volatile oils from Saururus cernuus L. and evaluation of anti-Trypanosoma cruzi activity |
title_full |
Molecular dereplication of volatile oils from Saururus cernuus L. and evaluation of anti-Trypanosoma cruzi activity |
title_fullStr |
Molecular dereplication of volatile oils from Saururus cernuus L. and evaluation of anti-Trypanosoma cruzi activity |
title_full_unstemmed |
Molecular dereplication of volatile oils from Saururus cernuus L. and evaluation of anti-Trypanosoma cruzi activity |
title_sort |
Molecular dereplication of volatile oils from Saururus cernuus L. and evaluation of anti-Trypanosoma cruzi activity |
author |
Brito,Juliana R |
author_facet |
Brito,Juliana R Costa-Silva,Thais A Londero,Vinicius S Baldim,João L Tempone,Andre G Ferreira,Edgard A Lago,João Henrique G |
author_role |
author |
author2 |
Costa-Silva,Thais A Londero,Vinicius S Baldim,João L Tempone,Andre G Ferreira,Edgard A Lago,João Henrique G |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Brito,Juliana R Costa-Silva,Thais A Londero,Vinicius S Baldim,João L Tempone,Andre G Ferreira,Edgard A Lago,João Henrique G |
dc.subject.por.fl_str_mv |
Saururus cernuus volatile oils terpenes safrol Trypanosoma cruzi. |
topic |
Saururus cernuus volatile oils terpenes safrol Trypanosoma cruzi. |
description |
The present study reports the molecular dereplication and the evaluation of anti-Trypanosoma cruzi activity of volatile oils from inflorescences, leaves, branches, and roots of Saururus cernuus L. (Saururaceae). Chemically, the oils showed the predominance of sesquiterpenes in inflorescences (64.04%) and branches (63.82%), with β-sesquiphellandrene (25.50%) and (E)-caryophyllene (22.40%) corresponding to the main constituents of each oil. Furthermore, it was possible to detect safrole as the most predominant compound in the leaves oil (49.09%). On the other hand, the oil from roots was mainly composed by monoterpenes (84.60%), with limonene in higher concentration (38.41%), followed by α-pinene (20.19%), and camphene (14.71%). The oils from inflorescences and branches displayed higher antitrypanosomal potency with EC50 values of 7.1 and 8.8 μg mL-1, respectively, followed by the oils from the roots and inflorescences, with EC50 values of 17.3 and 30.4 μg mL-1, respectively. Additionally, branches and inflorescences oils displayed no toxicity in mammalian NCTC cells (CC50 > 200 μg mL-1). Using two PLS-DA methods, it was possible to suggest that the anti-T. cruzi activity of the tested oils could be associated with the presence of β-sesquiphellandrene, safrole, β-elemene, and α-zingiberene whereas threo-austrobailignan-5, β-sesquiphellandrene, α-humulene, germacrene D, and bicyclogermacrene play a role in the cytotoxicity against NCTC cells. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422022000200159 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422022000200159 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.21577/0100-4042.20170811 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Química Nova v.45 n.2 2022 reponame:Química Nova (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Química Nova (Online) |
collection |
Química Nova (Online) |
repository.name.fl_str_mv |
Química Nova (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
quimicanova@sbq.org.br |
_version_ |
1750318121568174080 |