PREPARATIVE SEPARATION OF ISOQUINOLINE ALKALOIDS FROM PHELLODENDRI CORTEX BY PH-ZONE-REFINING COUNTER-CURRENT CHROMATOGRAPHY

Detalhes bibliográficos
Autor(a) principal: Wang,Daijie
Data de Publicação: 2018
Outros Autores: Tian,Shanming, Cui,Li, Yan,Huijiao, Song,Xiangyun, Liu,Yuguo, Xu,Fei, Ma,Junjian
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422018000300297
Resumo: pH-Zone-refining counter-current chromatography (PZRCCC) was successfully used for preparative separation isoquinoline alkaloids from Phellodendri Cortex. Two target compounds of berberine and palmatine were completely resolved by a two-phase solvent system chloroform/methanol/water (4:2:2, v/v), with 10 mmol L-1 HCl in upper stationary phase and 10 mmol L-1 TEA in lower organic phase. From 2.0 g of the crude alkaloids, palmatine (448 mg, 95.1%) and berberine (159 mg, 95.4%) were obtained in one-step PZRCCC separation. The chemical structures were identified by ESI-MS and NMR data. The results indicated that PZRCCC separation is an excellent preparative separation method for isoquinoline alkaloids from Phellodendri Cortex.
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spelling PREPARATIVE SEPARATION OF ISOQUINOLINE ALKALOIDS FROM PHELLODENDRI CORTEX BY PH-ZONE-REFINING COUNTER-CURRENT CHROMATOGRAPHYPhellodendri Cortexisoquinoline alkaloidspalmatineberberinepH-Zone-Refining Counter-Current ChromatographypH-Zone-refining counter-current chromatography (PZRCCC) was successfully used for preparative separation isoquinoline alkaloids from Phellodendri Cortex. Two target compounds of berberine and palmatine were completely resolved by a two-phase solvent system chloroform/methanol/water (4:2:2, v/v), with 10 mmol L-1 HCl in upper stationary phase and 10 mmol L-1 TEA in lower organic phase. From 2.0 g of the crude alkaloids, palmatine (448 mg, 95.1%) and berberine (159 mg, 95.4%) were obtained in one-step PZRCCC separation. The chemical structures were identified by ESI-MS and NMR data. The results indicated that PZRCCC separation is an excellent preparative separation method for isoquinoline alkaloids from Phellodendri Cortex.Sociedade Brasileira de Química2018-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422018000300297Química Nova v.41 n.3 2018reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.21577/0100-4042.20170184info:eu-repo/semantics/openAccessWang,DaijieTian,ShanmingCui,LiYan,HuijiaoSong,XiangyunLiu,YuguoXu,FeiMa,Junjianeng2018-04-23T00:00:00Zoai:scielo:S0100-40422018000300297Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2018-04-23T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv PREPARATIVE SEPARATION OF ISOQUINOLINE ALKALOIDS FROM PHELLODENDRI CORTEX BY PH-ZONE-REFINING COUNTER-CURRENT CHROMATOGRAPHY
title PREPARATIVE SEPARATION OF ISOQUINOLINE ALKALOIDS FROM PHELLODENDRI CORTEX BY PH-ZONE-REFINING COUNTER-CURRENT CHROMATOGRAPHY
spellingShingle PREPARATIVE SEPARATION OF ISOQUINOLINE ALKALOIDS FROM PHELLODENDRI CORTEX BY PH-ZONE-REFINING COUNTER-CURRENT CHROMATOGRAPHY
Wang,Daijie
Phellodendri Cortex
isoquinoline alkaloids
palmatine
berberine
pH-Zone-Refining Counter-Current Chromatography
title_short PREPARATIVE SEPARATION OF ISOQUINOLINE ALKALOIDS FROM PHELLODENDRI CORTEX BY PH-ZONE-REFINING COUNTER-CURRENT CHROMATOGRAPHY
title_full PREPARATIVE SEPARATION OF ISOQUINOLINE ALKALOIDS FROM PHELLODENDRI CORTEX BY PH-ZONE-REFINING COUNTER-CURRENT CHROMATOGRAPHY
title_fullStr PREPARATIVE SEPARATION OF ISOQUINOLINE ALKALOIDS FROM PHELLODENDRI CORTEX BY PH-ZONE-REFINING COUNTER-CURRENT CHROMATOGRAPHY
title_full_unstemmed PREPARATIVE SEPARATION OF ISOQUINOLINE ALKALOIDS FROM PHELLODENDRI CORTEX BY PH-ZONE-REFINING COUNTER-CURRENT CHROMATOGRAPHY
title_sort PREPARATIVE SEPARATION OF ISOQUINOLINE ALKALOIDS FROM PHELLODENDRI CORTEX BY PH-ZONE-REFINING COUNTER-CURRENT CHROMATOGRAPHY
author Wang,Daijie
author_facet Wang,Daijie
Tian,Shanming
Cui,Li
Yan,Huijiao
Song,Xiangyun
Liu,Yuguo
Xu,Fei
Ma,Junjian
author_role author
author2 Tian,Shanming
Cui,Li
Yan,Huijiao
Song,Xiangyun
Liu,Yuguo
Xu,Fei
Ma,Junjian
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Wang,Daijie
Tian,Shanming
Cui,Li
Yan,Huijiao
Song,Xiangyun
Liu,Yuguo
Xu,Fei
Ma,Junjian
dc.subject.por.fl_str_mv Phellodendri Cortex
isoquinoline alkaloids
palmatine
berberine
pH-Zone-Refining Counter-Current Chromatography
topic Phellodendri Cortex
isoquinoline alkaloids
palmatine
berberine
pH-Zone-Refining Counter-Current Chromatography
description pH-Zone-refining counter-current chromatography (PZRCCC) was successfully used for preparative separation isoquinoline alkaloids from Phellodendri Cortex. Two target compounds of berberine and palmatine were completely resolved by a two-phase solvent system chloroform/methanol/water (4:2:2, v/v), with 10 mmol L-1 HCl in upper stationary phase and 10 mmol L-1 TEA in lower organic phase. From 2.0 g of the crude alkaloids, palmatine (448 mg, 95.1%) and berberine (159 mg, 95.4%) were obtained in one-step PZRCCC separation. The chemical structures were identified by ESI-MS and NMR data. The results indicated that PZRCCC separation is an excellent preparative separation method for isoquinoline alkaloids from Phellodendri Cortex.
publishDate 2018
dc.date.none.fl_str_mv 2018-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422018000300297
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422018000300297
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.21577/0100-4042.20170184
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.41 n.3 2018
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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