Influence of air temperature on proteinase activity and beverage quality in Coffea arabica
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Botany |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042012000400009 |
Resumo: | Fruits were collected from trees of Coffea arabica cv. Obatã grown at Mococa and Adamantina in São Paulo State, Brazil, which are regions with marked differences in air temperature that produce coffee with distinct qualities. Mococa is a cooler location that produces high-quality coffee, whereas coffee from Adamantina is of lower quality. The amino acid and protein contents, amino acid profile, and proteinase activity and type in endosperm protein extracts were analysed. Proteinase genes were identified, and their expression was assayed. All results indicate that temperature plays a role in controlling proteinase activity in coffee endosperm. Proteinase activity was higher in the endosperm of immature fruits from Adamantina, which was correlated with higher amino acid content, changes in the amino acid profile, and increased gene expression. Cysteine proteinases were the main class of proteinases in the protein extracts. These data suggest that temperature plays an important role in coffee quality by altering nitrogen compound composition. |
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Brazilian Journal of Botany |
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Influence of air temperature on proteinase activity and beverage quality in Coffea arabicaamino acidscoffee qualitycysteine proteinaseendospermproteinFruits were collected from trees of Coffea arabica cv. Obatã grown at Mococa and Adamantina in São Paulo State, Brazil, which are regions with marked differences in air temperature that produce coffee with distinct qualities. Mococa is a cooler location that produces high-quality coffee, whereas coffee from Adamantina is of lower quality. The amino acid and protein contents, amino acid profile, and proteinase activity and type in endosperm protein extracts were analysed. Proteinase genes were identified, and their expression was assayed. All results indicate that temperature plays a role in controlling proteinase activity in coffee endosperm. Proteinase activity was higher in the endosperm of immature fruits from Adamantina, which was correlated with higher amino acid content, changes in the amino acid profile, and increased gene expression. Cysteine proteinases were the main class of proteinases in the protein extracts. These data suggest that temperature plays an important role in coffee quality by altering nitrogen compound composition.Sociedade Botânica de São Paulo2012-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042012000400009Brazilian Journal of Botany v.35 n.4 2012reponame:Brazilian Journal of Botanyinstname:Sociedade Botânica de São Paulo (SBSP)instacron:SBSP10.1590/S0100-84042012000400009info:eu-repo/semantics/openAccessAbreu,Hellen Marília Couto deNobile,Paula MacedoShimizu,Milton MassaoYamamoto,Paula YuriSilva,Emerson AlvesColombo,Carlos AugustoMazzafera,Pauloeng2015-03-11T00:00:00Zoai:scielo:S0100-84042012000400009Revistahttps://www.scielo.br/j/rbb/ONGhttps://old.scielo.br/oai/scielo-oai.phpbrazbot@gmail.com||brazbot@gmail.com1806-99590100-8404opendoar:2015-03-11T00:00Brazilian Journal of Botany - Sociedade Botânica de São Paulo (SBSP)false |
dc.title.none.fl_str_mv |
Influence of air temperature on proteinase activity and beverage quality in Coffea arabica |
title |
Influence of air temperature on proteinase activity and beverage quality in Coffea arabica |
spellingShingle |
Influence of air temperature on proteinase activity and beverage quality in Coffea arabica Abreu,Hellen Marília Couto de amino acids coffee quality cysteine proteinase endosperm protein |
title_short |
Influence of air temperature on proteinase activity and beverage quality in Coffea arabica |
title_full |
Influence of air temperature on proteinase activity and beverage quality in Coffea arabica |
title_fullStr |
Influence of air temperature on proteinase activity and beverage quality in Coffea arabica |
title_full_unstemmed |
Influence of air temperature on proteinase activity and beverage quality in Coffea arabica |
title_sort |
Influence of air temperature on proteinase activity and beverage quality in Coffea arabica |
author |
Abreu,Hellen Marília Couto de |
author_facet |
Abreu,Hellen Marília Couto de Nobile,Paula Macedo Shimizu,Milton Massao Yamamoto,Paula Yuri Silva,Emerson Alves Colombo,Carlos Augusto Mazzafera,Paulo |
author_role |
author |
author2 |
Nobile,Paula Macedo Shimizu,Milton Massao Yamamoto,Paula Yuri Silva,Emerson Alves Colombo,Carlos Augusto Mazzafera,Paulo |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Abreu,Hellen Marília Couto de Nobile,Paula Macedo Shimizu,Milton Massao Yamamoto,Paula Yuri Silva,Emerson Alves Colombo,Carlos Augusto Mazzafera,Paulo |
dc.subject.por.fl_str_mv |
amino acids coffee quality cysteine proteinase endosperm protein |
topic |
amino acids coffee quality cysteine proteinase endosperm protein |
description |
Fruits were collected from trees of Coffea arabica cv. Obatã grown at Mococa and Adamantina in São Paulo State, Brazil, which are regions with marked differences in air temperature that produce coffee with distinct qualities. Mococa is a cooler location that produces high-quality coffee, whereas coffee from Adamantina is of lower quality. The amino acid and protein contents, amino acid profile, and proteinase activity and type in endosperm protein extracts were analysed. Proteinase genes were identified, and their expression was assayed. All results indicate that temperature plays a role in controlling proteinase activity in coffee endosperm. Proteinase activity was higher in the endosperm of immature fruits from Adamantina, which was correlated with higher amino acid content, changes in the amino acid profile, and increased gene expression. Cysteine proteinases were the main class of proteinases in the protein extracts. These data suggest that temperature plays an important role in coffee quality by altering nitrogen compound composition. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042012000400009 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042012000400009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-84042012000400009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Botânica de São Paulo |
publisher.none.fl_str_mv |
Sociedade Botânica de São Paulo |
dc.source.none.fl_str_mv |
Brazilian Journal of Botany v.35 n.4 2012 reponame:Brazilian Journal of Botany instname:Sociedade Botânica de São Paulo (SBSP) instacron:SBSP |
instname_str |
Sociedade Botânica de São Paulo (SBSP) |
instacron_str |
SBSP |
institution |
SBSP |
reponame_str |
Brazilian Journal of Botany |
collection |
Brazilian Journal of Botany |
repository.name.fl_str_mv |
Brazilian Journal of Botany - Sociedade Botânica de São Paulo (SBSP) |
repository.mail.fl_str_mv |
brazbot@gmail.com||brazbot@gmail.com |
_version_ |
1754734840786190336 |