In vitro culture of Phyllanthus stipulatus (Euphorbiaceae)
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Botany |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042001000100003 |
Resumo: | An efficient micropropagation protocol was developed for the medicinal plant Phyllanthus stipulatus (Euphorbiaceae) using nodal segments for axillary shoot proliferation. Maximum multiplication rates (8-9 shoots per explant) was achieved on MS media supplemented with either 2.5-5.0 muM IBA. The best basal media for axillary shoot proliferation when 0.62 muM BA was supplemented were MS, MS/2 and AR (4-5 shoots per explant). Rooting was achieved with 100% of the microshoots on MS medium without growth regulators. Regenerated plants were successfully acclimatized and about 88% of plantlets survived under ex vitro conditions. Flowering was observed in 81% of the ex vitro grown plantlets after 12 weeks of acclimatization. High frequency callus initiation and growth was achieved when nodal segment explants were inoculated either in the vertical position, in the light on MS medium supplemented with 5.0 muM NAA or horizontally oriented, in the dark on MS supplemented with 5.0 muM NAA or 1.25-5.0 muM BA or 2iP. Root cultures were successfully established on MS medium containing 1.1 muM NAA. The optimized micropropagation, callus and root culture protocols offer the possibility to use cell/organ culture techniques for vegetative propagation and secondary metabolism studies. |
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Brazilian Journal of Botany |
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In vitro culture of Phyllanthus stipulatus (Euphorbiaceae)Shoot cultureacclimatizationex-vitro floweringcallus cultureroot cultureAn efficient micropropagation protocol was developed for the medicinal plant Phyllanthus stipulatus (Euphorbiaceae) using nodal segments for axillary shoot proliferation. Maximum multiplication rates (8-9 shoots per explant) was achieved on MS media supplemented with either 2.5-5.0 muM IBA. The best basal media for axillary shoot proliferation when 0.62 muM BA was supplemented were MS, MS/2 and AR (4-5 shoots per explant). Rooting was achieved with 100% of the microshoots on MS medium without growth regulators. Regenerated plants were successfully acclimatized and about 88% of plantlets survived under ex vitro conditions. Flowering was observed in 81% of the ex vitro grown plantlets after 12 weeks of acclimatization. High frequency callus initiation and growth was achieved when nodal segment explants were inoculated either in the vertical position, in the light on MS medium supplemented with 5.0 muM NAA or horizontally oriented, in the dark on MS supplemented with 5.0 muM NAA or 1.25-5.0 muM BA or 2iP. Root cultures were successfully established on MS medium containing 1.1 muM NAA. The optimized micropropagation, callus and root culture protocols offer the possibility to use cell/organ culture techniques for vegetative propagation and secondary metabolism studies.Sociedade Botânica de São Paulo2001-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042001000100003Brazilian Journal of Botany v.24 n.1 2001reponame:Brazilian Journal of Botanyinstname:Sociedade Botânica de São Paulo (SBSP)instacron:SBSP10.1590/S0100-84042001000100003info:eu-repo/semantics/openAccessCATAPAN,ELIZABETEOTUKI,MICHEL FLEITHVIANA,ANA MARIAeng2001-05-03T00:00:00Zoai:scielo:S0100-84042001000100003Revistahttps://www.scielo.br/j/rbb/ONGhttps://old.scielo.br/oai/scielo-oai.phpbrazbot@gmail.com||brazbot@gmail.com1806-99590100-8404opendoar:2001-05-03T00:00Brazilian Journal of Botany - Sociedade Botânica de São Paulo (SBSP)false |
dc.title.none.fl_str_mv |
In vitro culture of Phyllanthus stipulatus (Euphorbiaceae) |
title |
In vitro culture of Phyllanthus stipulatus (Euphorbiaceae) |
spellingShingle |
In vitro culture of Phyllanthus stipulatus (Euphorbiaceae) CATAPAN,ELIZABETE Shoot culture acclimatization ex-vitro flowering callus culture root culture |
title_short |
In vitro culture of Phyllanthus stipulatus (Euphorbiaceae) |
title_full |
In vitro culture of Phyllanthus stipulatus (Euphorbiaceae) |
title_fullStr |
In vitro culture of Phyllanthus stipulatus (Euphorbiaceae) |
title_full_unstemmed |
In vitro culture of Phyllanthus stipulatus (Euphorbiaceae) |
title_sort |
In vitro culture of Phyllanthus stipulatus (Euphorbiaceae) |
author |
CATAPAN,ELIZABETE |
author_facet |
CATAPAN,ELIZABETE OTUKI,MICHEL FLEITH VIANA,ANA MARIA |
author_role |
author |
author2 |
OTUKI,MICHEL FLEITH VIANA,ANA MARIA |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
CATAPAN,ELIZABETE OTUKI,MICHEL FLEITH VIANA,ANA MARIA |
dc.subject.por.fl_str_mv |
Shoot culture acclimatization ex-vitro flowering callus culture root culture |
topic |
Shoot culture acclimatization ex-vitro flowering callus culture root culture |
description |
An efficient micropropagation protocol was developed for the medicinal plant Phyllanthus stipulatus (Euphorbiaceae) using nodal segments for axillary shoot proliferation. Maximum multiplication rates (8-9 shoots per explant) was achieved on MS media supplemented with either 2.5-5.0 muM IBA. The best basal media for axillary shoot proliferation when 0.62 muM BA was supplemented were MS, MS/2 and AR (4-5 shoots per explant). Rooting was achieved with 100% of the microshoots on MS medium without growth regulators. Regenerated plants were successfully acclimatized and about 88% of plantlets survived under ex vitro conditions. Flowering was observed in 81% of the ex vitro grown plantlets after 12 weeks of acclimatization. High frequency callus initiation and growth was achieved when nodal segment explants were inoculated either in the vertical position, in the light on MS medium supplemented with 5.0 muM NAA or horizontally oriented, in the dark on MS supplemented with 5.0 muM NAA or 1.25-5.0 muM BA or 2iP. Root cultures were successfully established on MS medium containing 1.1 muM NAA. The optimized micropropagation, callus and root culture protocols offer the possibility to use cell/organ culture techniques for vegetative propagation and secondary metabolism studies. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042001000100003 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042001000100003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-84042001000100003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Botânica de São Paulo |
publisher.none.fl_str_mv |
Sociedade Botânica de São Paulo |
dc.source.none.fl_str_mv |
Brazilian Journal of Botany v.24 n.1 2001 reponame:Brazilian Journal of Botany instname:Sociedade Botânica de São Paulo (SBSP) instacron:SBSP |
instname_str |
Sociedade Botânica de São Paulo (SBSP) |
instacron_str |
SBSP |
institution |
SBSP |
reponame_str |
Brazilian Journal of Botany |
collection |
Brazilian Journal of Botany |
repository.name.fl_str_mv |
Brazilian Journal of Botany - Sociedade Botânica de São Paulo (SBSP) |
repository.mail.fl_str_mv |
brazbot@gmail.com||brazbot@gmail.com |
_version_ |
1754734837734834176 |