Tumor banks: the cornerstone of basic research in urology
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | International Braz J Urol (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382010000300012 |
Resumo: | PURPOSE: Tumor banks have the primary responsibility for collecting, cataloging, storing and disseminating samples of tissues, cells and fluids, which are used by researchers to identify diagnostic molecular markers, prognostic indicators and therapeutic targets. The objective of this review was to describe a simple, reliable and reproducible protocol for obtaining and storing samples of urological tumors. MATERIALS AND METHODS: Urogenital tumor tissues were collected by the surgeons from the Urology Division of University of Sao Paulo Medical School. The obtained surgical specimens were immediately placed in liquid nitrogen, dry ice or in a tube containing RNAlater ®, and then stored by cryopreservation (-80°C). A mirror fragment was fixed in 10% formalin processed routinely and embedded in Paraplast®. RESULTS: We developed a protocol for the collection, cataloging, storage, conservation and use of tumor samples. During a period of one year the Urological Tumor Bank of the Urology Division stored 274 samples of prostate, bladder, kidney, penis and testicle tumors of different histological types, 74 urine and 271 serum samples. CONCLUSIONS: Having biological materials characterized and available along with the clinical patient information provides an integrated portrait of the patients and their diseases facilitating advances in molecular biology. It also promotes the development of translational research improving methods of diagnosis and cancer treatment. |
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Tumor banks: the cornerstone of basic research in urologybiological specimen banksurogenital neoplasmstissue bankspathologymolecular biologyPURPOSE: Tumor banks have the primary responsibility for collecting, cataloging, storing and disseminating samples of tissues, cells and fluids, which are used by researchers to identify diagnostic molecular markers, prognostic indicators and therapeutic targets. The objective of this review was to describe a simple, reliable and reproducible protocol for obtaining and storing samples of urological tumors. MATERIALS AND METHODS: Urogenital tumor tissues were collected by the surgeons from the Urology Division of University of Sao Paulo Medical School. The obtained surgical specimens were immediately placed in liquid nitrogen, dry ice or in a tube containing RNAlater ®, and then stored by cryopreservation (-80°C). A mirror fragment was fixed in 10% formalin processed routinely and embedded in Paraplast®. RESULTS: We developed a protocol for the collection, cataloging, storage, conservation and use of tumor samples. During a period of one year the Urological Tumor Bank of the Urology Division stored 274 samples of prostate, bladder, kidney, penis and testicle tumors of different histological types, 74 urine and 271 serum samples. CONCLUSIONS: Having biological materials characterized and available along with the clinical patient information provides an integrated portrait of the patients and their diseases facilitating advances in molecular biology. It also promotes the development of translational research improving methods of diagnosis and cancer treatment.Sociedade Brasileira de Urologia2010-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382010000300012International braz j urol v.36 n.3 2010reponame:International Braz J Urol (Online)instname:Sociedade Brasileira de Urologia (SBU)instacron:SBU10.1590/S1677-55382010000300012info:eu-repo/semantics/openAccessReis,Sabrina T.Feitosa,Emanoela B.Pontes-Junior,JoseMarin,Camila C.Abe,Daniel k.Crippa,AlexandreAntunes,Alberto A.Nesrallah,Adriano J.Oliveira,Luiz C.Ribeiro-Filho,Leopoldo A.Srougi,MiguelLeite,Katia R. M.Dall’Oglio,Marcos F.eng2010-08-12T00:00:00Zoai:scielo:S1677-55382010000300012Revistahttp://www.brazjurol.com.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||brazjurol@brazjurol.com.br1677-61191677-5538opendoar:2010-08-12T00:00International Braz J Urol (Online) - Sociedade Brasileira de Urologia (SBU)false |
dc.title.none.fl_str_mv |
Tumor banks: the cornerstone of basic research in urology |
title |
Tumor banks: the cornerstone of basic research in urology |
spellingShingle |
Tumor banks: the cornerstone of basic research in urology Reis,Sabrina T. biological specimen banks urogenital neoplasms tissue banks pathology molecular biology |
title_short |
Tumor banks: the cornerstone of basic research in urology |
title_full |
Tumor banks: the cornerstone of basic research in urology |
title_fullStr |
Tumor banks: the cornerstone of basic research in urology |
title_full_unstemmed |
Tumor banks: the cornerstone of basic research in urology |
title_sort |
Tumor banks: the cornerstone of basic research in urology |
author |
Reis,Sabrina T. |
author_facet |
Reis,Sabrina T. Feitosa,Emanoela B. Pontes-Junior,Jose Marin,Camila C. Abe,Daniel k. Crippa,Alexandre Antunes,Alberto A. Nesrallah,Adriano J. Oliveira,Luiz C. Ribeiro-Filho,Leopoldo A. Srougi,Miguel Leite,Katia R. M. Dall’Oglio,Marcos F. |
author_role |
author |
author2 |
Feitosa,Emanoela B. Pontes-Junior,Jose Marin,Camila C. Abe,Daniel k. Crippa,Alexandre Antunes,Alberto A. Nesrallah,Adriano J. Oliveira,Luiz C. Ribeiro-Filho,Leopoldo A. Srougi,Miguel Leite,Katia R. M. Dall’Oglio,Marcos F. |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Reis,Sabrina T. Feitosa,Emanoela B. Pontes-Junior,Jose Marin,Camila C. Abe,Daniel k. Crippa,Alexandre Antunes,Alberto A. Nesrallah,Adriano J. Oliveira,Luiz C. Ribeiro-Filho,Leopoldo A. Srougi,Miguel Leite,Katia R. M. Dall’Oglio,Marcos F. |
dc.subject.por.fl_str_mv |
biological specimen banks urogenital neoplasms tissue banks pathology molecular biology |
topic |
biological specimen banks urogenital neoplasms tissue banks pathology molecular biology |
description |
PURPOSE: Tumor banks have the primary responsibility for collecting, cataloging, storing and disseminating samples of tissues, cells and fluids, which are used by researchers to identify diagnostic molecular markers, prognostic indicators and therapeutic targets. The objective of this review was to describe a simple, reliable and reproducible protocol for obtaining and storing samples of urological tumors. MATERIALS AND METHODS: Urogenital tumor tissues were collected by the surgeons from the Urology Division of University of Sao Paulo Medical School. The obtained surgical specimens were immediately placed in liquid nitrogen, dry ice or in a tube containing RNAlater ®, and then stored by cryopreservation (-80°C). A mirror fragment was fixed in 10% formalin processed routinely and embedded in Paraplast®. RESULTS: We developed a protocol for the collection, cataloging, storage, conservation and use of tumor samples. During a period of one year the Urological Tumor Bank of the Urology Division stored 274 samples of prostate, bladder, kidney, penis and testicle tumors of different histological types, 74 urine and 271 serum samples. CONCLUSIONS: Having biological materials characterized and available along with the clinical patient information provides an integrated portrait of the patients and their diseases facilitating advances in molecular biology. It also promotes the development of translational research improving methods of diagnosis and cancer treatment. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382010000300012 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382010000300012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1677-55382010000300012 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Urologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Urologia |
dc.source.none.fl_str_mv |
International braz j urol v.36 n.3 2010 reponame:International Braz J Urol (Online) instname:Sociedade Brasileira de Urologia (SBU) instacron:SBU |
instname_str |
Sociedade Brasileira de Urologia (SBU) |
instacron_str |
SBU |
institution |
SBU |
reponame_str |
International Braz J Urol (Online) |
collection |
International Braz J Urol (Online) |
repository.name.fl_str_mv |
International Braz J Urol (Online) - Sociedade Brasileira de Urologia (SBU) |
repository.mail.fl_str_mv |
||brazjurol@brazjurol.com.br |
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1750318071668539392 |