Effect of ischemia preconditioning on renal ischemia/reperfusion injury in rats

• Main Author: Fan,Lian-hui
• Publication Date: 2012
• Other Authors: He,Long, Cao,Zhi-qiang, Xiang,Jun, Liu,Long
• Format: Article
• Language: eng
• Source: International Braz J Urol (Online)
• Download full: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382012000600842
• topic: Ischemic Preconditioning; Intercellular Adhesion Molecule-1; P-Selectin; E-Selectin; Ischemia; Reperfusion Injury

Objective To study the effect of ischemia preconditioning (IP) on renal ischemia/reperfusion (I/R)-associated functional injury and expression of renal adhesion molecules in rats. Materials and Methods The ischemia preconditioning plan adopted in this experiment involved renal warm ischemia for 6 min. and blood flow for 4 min., repeated four times. The Wistar rat kidneys used for warm ischemia preconditioning were subjected to 60 min of renal warm ischemia followed by reperfusion. The rat kidneys with ischemia/reperfusion were compared with the ischemia preconditioning group to observe rat renal function and changes in the expression of renal adhesion molecules ICAM-1, P--Selectin, and E-Selectin. Results The expression of rat renal adhesion molecules (ICAM-1, P-Selectin, and E-Selectin) with ischemia preconditioning was significantly lower than that of the ischemia/reperfusion group. Serum creatinine was significantly lower than that in the ischemia/reperfusion group after 48 hours. Conclusions Ischemia preconditioning has a protective effect on renal function. Reduced expression of renal adhesion molecules is likely a mechanism involved in the observed protection.