Micro RNA expression and prognosis in low-grade non-invasive urothelial carcinoma
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | International Braz J Urol (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382014000500644 |
Resumo: | Purpose To analyze a possible correlation between a miRNA expression profile and important prognostic factors for pTa urothelial carcinomas (UC), including tumor size, multiplicity and episodes of recurrence. Materials and Methods Thirty low-grade non-invasive pTa bladder UC from patients submitted to transurethral resection were studied, in a mean follow-up of 17.7 months. As controls, we used normal bladder tissue from five patients submitted to retropubic prostatectomy to treat benign prostatic hyperplasia. Extraction, cDNA and amplification were performed for 14 miRNAs (miR-100, -10a, -21, -205, -let7c, -143, -145, -221, -223, -15a, -16, -199a and -452) using specific kits, and RNU-43 and -48 were used as endogenous controls. Statistical tests were used to compare tumor size, multiplicity and episodes of recurrence with miRNAs expression profiles. Results There was a marginal correlation between multiplicity and miR-let7c over-expression. For all others miRNA no correlation between their expression and prognostic factors was found. Conclusion We did not find differences for miRNAs expression profiles associated with prognostic factors in tumor group studied. The majority of miRNAs are down-regulated, except miR-10a, over-expressed in most of cases, seeming to have increased levels in tumor with more unfavorable prognostic factors. More studies are needed in order to find a miRNA profile able to provide prognosis in pTa UC to be used in clinical practice. |
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Micro RNA expression and prognosis in low-grade non-invasive urothelial carcinomaMicroRNAsUrinary Bladder NeoplasmsPrognosisMolecular Biology Purpose To analyze a possible correlation between a miRNA expression profile and important prognostic factors for pTa urothelial carcinomas (UC), including tumor size, multiplicity and episodes of recurrence. Materials and Methods Thirty low-grade non-invasive pTa bladder UC from patients submitted to transurethral resection were studied, in a mean follow-up of 17.7 months. As controls, we used normal bladder tissue from five patients submitted to retropubic prostatectomy to treat benign prostatic hyperplasia. Extraction, cDNA and amplification were performed for 14 miRNAs (miR-100, -10a, -21, -205, -let7c, -143, -145, -221, -223, -15a, -16, -199a and -452) using specific kits, and RNU-43 and -48 were used as endogenous controls. Statistical tests were used to compare tumor size, multiplicity and episodes of recurrence with miRNAs expression profiles. Results There was a marginal correlation between multiplicity and miR-let7c over-expression. For all others miRNA no correlation between their expression and prognostic factors was found. Conclusion We did not find differences for miRNAs expression profiles associated with prognostic factors in tumor group studied. The majority of miRNAs are down-regulated, except miR-10a, over-expressed in most of cases, seeming to have increased levels in tumor with more unfavorable prognostic factors. More studies are needed in order to find a miRNA profile able to provide prognosis in pTa UC to be used in clinical practice. Sociedade Brasileira de Urologia2014-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382014000500644International braz j urol v.40 n.5 2014reponame:International Braz J Urol (Online)instname:Sociedade Brasileira de Urologia (SBU)instacron:SBU10.1590/S1677-5538.IBJU.2014.05.09info:eu-repo/semantics/openAccessDip,NelsonReis,Sabrina T.Abe,Daniel K.Viana,Nayara I.Morais,Denis R.Moura,Caio M.Katz,BetinaSilva,Iran A.Srougi,MiguelLeite,Katia R. M.eng2014-12-11T00:00:00Zoai:scielo:S1677-55382014000500644Revistahttp://www.brazjurol.com.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||brazjurol@brazjurol.com.br1677-61191677-5538opendoar:2014-12-11T00:00International Braz J Urol (Online) - Sociedade Brasileira de Urologia (SBU)false |
dc.title.none.fl_str_mv |
Micro RNA expression and prognosis in low-grade non-invasive urothelial carcinoma |
title |
Micro RNA expression and prognosis in low-grade non-invasive urothelial carcinoma |
spellingShingle |
Micro RNA expression and prognosis in low-grade non-invasive urothelial carcinoma Dip,Nelson MicroRNAs Urinary Bladder Neoplasms Prognosis Molecular Biology |
title_short |
Micro RNA expression and prognosis in low-grade non-invasive urothelial carcinoma |
title_full |
Micro RNA expression and prognosis in low-grade non-invasive urothelial carcinoma |
title_fullStr |
Micro RNA expression and prognosis in low-grade non-invasive urothelial carcinoma |
title_full_unstemmed |
Micro RNA expression and prognosis in low-grade non-invasive urothelial carcinoma |
title_sort |
Micro RNA expression and prognosis in low-grade non-invasive urothelial carcinoma |
author |
Dip,Nelson |
author_facet |
Dip,Nelson Reis,Sabrina T. Abe,Daniel K. Viana,Nayara I. Morais,Denis R. Moura,Caio M. Katz,Betina Silva,Iran A. Srougi,Miguel Leite,Katia R. M. |
author_role |
author |
author2 |
Reis,Sabrina T. Abe,Daniel K. Viana,Nayara I. Morais,Denis R. Moura,Caio M. Katz,Betina Silva,Iran A. Srougi,Miguel Leite,Katia R. M. |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Dip,Nelson Reis,Sabrina T. Abe,Daniel K. Viana,Nayara I. Morais,Denis R. Moura,Caio M. Katz,Betina Silva,Iran A. Srougi,Miguel Leite,Katia R. M. |
dc.subject.por.fl_str_mv |
MicroRNAs Urinary Bladder Neoplasms Prognosis Molecular Biology |
topic |
MicroRNAs Urinary Bladder Neoplasms Prognosis Molecular Biology |
description |
Purpose To analyze a possible correlation between a miRNA expression profile and important prognostic factors for pTa urothelial carcinomas (UC), including tumor size, multiplicity and episodes of recurrence. Materials and Methods Thirty low-grade non-invasive pTa bladder UC from patients submitted to transurethral resection were studied, in a mean follow-up of 17.7 months. As controls, we used normal bladder tissue from five patients submitted to retropubic prostatectomy to treat benign prostatic hyperplasia. Extraction, cDNA and amplification were performed for 14 miRNAs (miR-100, -10a, -21, -205, -let7c, -143, -145, -221, -223, -15a, -16, -199a and -452) using specific kits, and RNU-43 and -48 were used as endogenous controls. Statistical tests were used to compare tumor size, multiplicity and episodes of recurrence with miRNAs expression profiles. Results There was a marginal correlation between multiplicity and miR-let7c over-expression. For all others miRNA no correlation between their expression and prognostic factors was found. Conclusion We did not find differences for miRNAs expression profiles associated with prognostic factors in tumor group studied. The majority of miRNAs are down-regulated, except miR-10a, over-expressed in most of cases, seeming to have increased levels in tumor with more unfavorable prognostic factors. More studies are needed in order to find a miRNA profile able to provide prognosis in pTa UC to be used in clinical practice. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382014000500644 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382014000500644 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1677-5538.IBJU.2014.05.09 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Urologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Urologia |
dc.source.none.fl_str_mv |
International braz j urol v.40 n.5 2014 reponame:International Braz J Urol (Online) instname:Sociedade Brasileira de Urologia (SBU) instacron:SBU |
instname_str |
Sociedade Brasileira de Urologia (SBU) |
instacron_str |
SBU |
institution |
SBU |
reponame_str |
International Braz J Urol (Online) |
collection |
International Braz J Urol (Online) |
repository.name.fl_str_mv |
International Braz J Urol (Online) - Sociedade Brasileira de Urologia (SBU) |
repository.mail.fl_str_mv |
||brazjurol@brazjurol.com.br |
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1750318073731088384 |