Expression of VEGF and collagen using a latex biomembrane as bladder replacement in rabbits
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | International Braz J Urol (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382012000400014 |
Resumo: | OBJECTIVE: To investigate the VEGF expression and collagen deposition using a latex biomembrane as bladder replacement in rabbits. MATERIALS AND METHODS: After partial cystectomy, a patch of a non-vulcanized latex biomembrane (2 x 2 cm) was sewn to the bladder of rabbits with 5/0 monofilament polydioxanone sulfate sutures in a watertight manner. Groups of 5 animals were killed at 15, 45 and 90 days after surgery and the bladder was removed. Sections of 5µm were cut and stained with picrosirius-red in order to estimate the amount of extracellular matrix in the graft. To confirm the presence of VEGF in tissues, protein expression was determined by immunohistochemistry. RESULTS: No death, urinary leakage or graft extrusion occurred in any group. All bladders showed a spherical shape. A progressive reduction in the amount of collagen occurred in the graft area and was negatively and linearly correlated with time (p < 0.001). VEGF expression was higher in grafted areas when compared to controls at 15 and 45 days after surgery and decreased with time (p < 0.001). CONCLUSION: The latex biomembrane as a matrix for partial bladder replacement in rabbits promotes temporary collagen deposition and stimulates the angiogenic process. |
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International Braz J Urol (Online) |
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Expression of VEGF and collagen using a latex biomembrane as bladder replacement in rabbitsLatexCollagenUrinary TractTissue-engineeringOBJECTIVE: To investigate the VEGF expression and collagen deposition using a latex biomembrane as bladder replacement in rabbits. MATERIALS AND METHODS: After partial cystectomy, a patch of a non-vulcanized latex biomembrane (2 x 2 cm) was sewn to the bladder of rabbits with 5/0 monofilament polydioxanone sulfate sutures in a watertight manner. Groups of 5 animals were killed at 15, 45 and 90 days after surgery and the bladder was removed. Sections of 5µm were cut and stained with picrosirius-red in order to estimate the amount of extracellular matrix in the graft. To confirm the presence of VEGF in tissues, protein expression was determined by immunohistochemistry. RESULTS: No death, urinary leakage or graft extrusion occurred in any group. All bladders showed a spherical shape. A progressive reduction in the amount of collagen occurred in the graft area and was negatively and linearly correlated with time (p < 0.001). VEGF expression was higher in grafted areas when compared to controls at 15 and 45 days after surgery and decreased with time (p < 0.001). CONCLUSION: The latex biomembrane as a matrix for partial bladder replacement in rabbits promotes temporary collagen deposition and stimulates the angiogenic process.Sociedade Brasileira de Urologia2012-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382012000400014International braz j urol v.38 n.4 2012reponame:International Braz J Urol (Online)instname:Sociedade Brasileira de Urologia (SBU)instacron:SBU10.1590/S1677-55382012000400014info:eu-repo/semantics/openAccessDomingos,André Luís AlonsoGarcia,Sérgio BrittoBessa Jr.,José deCassini,Marcelo FerreiraMolina,Carlos Augusto FernandesTucci Junior,Silvioeng2012-09-20T00:00:00Zoai:scielo:S1677-55382012000400014Revistahttp://www.brazjurol.com.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||brazjurol@brazjurol.com.br1677-61191677-5538opendoar:2012-09-20T00:00International Braz J Urol (Online) - Sociedade Brasileira de Urologia (SBU)false |
dc.title.none.fl_str_mv |
Expression of VEGF and collagen using a latex biomembrane as bladder replacement in rabbits |
title |
Expression of VEGF and collagen using a latex biomembrane as bladder replacement in rabbits |
spellingShingle |
Expression of VEGF and collagen using a latex biomembrane as bladder replacement in rabbits Domingos,André Luís Alonso Latex Collagen Urinary Tract Tissue-engineering |
title_short |
Expression of VEGF and collagen using a latex biomembrane as bladder replacement in rabbits |
title_full |
Expression of VEGF and collagen using a latex biomembrane as bladder replacement in rabbits |
title_fullStr |
Expression of VEGF and collagen using a latex biomembrane as bladder replacement in rabbits |
title_full_unstemmed |
Expression of VEGF and collagen using a latex biomembrane as bladder replacement in rabbits |
title_sort |
Expression of VEGF and collagen using a latex biomembrane as bladder replacement in rabbits |
author |
Domingos,André Luís Alonso |
author_facet |
Domingos,André Luís Alonso Garcia,Sérgio Britto Bessa Jr.,José de Cassini,Marcelo Ferreira Molina,Carlos Augusto Fernandes Tucci Junior,Silvio |
author_role |
author |
author2 |
Garcia,Sérgio Britto Bessa Jr.,José de Cassini,Marcelo Ferreira Molina,Carlos Augusto Fernandes Tucci Junior,Silvio |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Domingos,André Luís Alonso Garcia,Sérgio Britto Bessa Jr.,José de Cassini,Marcelo Ferreira Molina,Carlos Augusto Fernandes Tucci Junior,Silvio |
dc.subject.por.fl_str_mv |
Latex Collagen Urinary Tract Tissue-engineering |
topic |
Latex Collagen Urinary Tract Tissue-engineering |
description |
OBJECTIVE: To investigate the VEGF expression and collagen deposition using a latex biomembrane as bladder replacement in rabbits. MATERIALS AND METHODS: After partial cystectomy, a patch of a non-vulcanized latex biomembrane (2 x 2 cm) was sewn to the bladder of rabbits with 5/0 monofilament polydioxanone sulfate sutures in a watertight manner. Groups of 5 animals were killed at 15, 45 and 90 days after surgery and the bladder was removed. Sections of 5µm were cut and stained with picrosirius-red in order to estimate the amount of extracellular matrix in the graft. To confirm the presence of VEGF in tissues, protein expression was determined by immunohistochemistry. RESULTS: No death, urinary leakage or graft extrusion occurred in any group. All bladders showed a spherical shape. A progressive reduction in the amount of collagen occurred in the graft area and was negatively and linearly correlated with time (p < 0.001). VEGF expression was higher in grafted areas when compared to controls at 15 and 45 days after surgery and decreased with time (p < 0.001). CONCLUSION: The latex biomembrane as a matrix for partial bladder replacement in rabbits promotes temporary collagen deposition and stimulates the angiogenic process. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382012000400014 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382012000400014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1677-55382012000400014 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Urologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Urologia |
dc.source.none.fl_str_mv |
International braz j urol v.38 n.4 2012 reponame:International Braz J Urol (Online) instname:Sociedade Brasileira de Urologia (SBU) instacron:SBU |
instname_str |
Sociedade Brasileira de Urologia (SBU) |
instacron_str |
SBU |
institution |
SBU |
reponame_str |
International Braz J Urol (Online) |
collection |
International Braz J Urol (Online) |
repository.name.fl_str_mv |
International Braz J Urol (Online) - Sociedade Brasileira de Urologia (SBU) |
repository.mail.fl_str_mv |
||brazjurol@brazjurol.com.br |
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1750318072796807168 |