New findings of intestinal alkaline phosphatase: effects on intestinal and organ health of piglets challenged with ETEC F4 (K88)

Detalhes bibliográficos
Autor(a) principal: Genova,Jansller Luiz
Data de Publicação: 2022
Outros Autores: Melo,Antonio Diego Brandão, Rupolo,Paulo Evaristo, Macedo,Renata Ernlund Freitas de, Engracia Filho,Jair Rodini, Carvalho,Silvana Teixeira, Faucitano,Luigi, Costa,Leandro Batista, Carvalho,Paulo Levi de Oliveira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Zootecnia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982022000100609
Resumo: ABSTRACT The aim of this study was to assess the addition of intestinal alkaline phosphatase (IAP) to diets on the count of bacterial populations, pH of digestive organ contents, histopathological description, proinflammatory markers, hepatic glycogen reserve, and diarrhoea incidence of piglets challenged with Escherichia coli. Sixty-four crossbred piglets (7.16±0.28 kg body weight, 25-days-old) were assigned to four treatments in a completely randomised block design: negative control (NC), NC + antibiotic (ANT), NC + 15 mg IAP, or NC + 30 mg IAP kg−1 of diet, eight replications of two piglets per experimental unit. All piglets were orally challenged with 6 mL of a solution containing enterotoxigenic Escherichia coli K88 at 106 CFU mL−1 at 15 days of experimentation. The study lasted for 19 days. At the end of the experimental period, the piglets were slaughtered (six animals per treatment). Enterobacteriaceae in caecum and colon was lower in piglets on 30 mg IAP than with ANT and NC, ANT or 15 mg IAP, respectively. Enterobacteriaceae adhered to the mesenteric lymph nodes (MLN) was greater in piglets fed ANT than the other treatments. Lactic acid bacteria (LAB) count in caecum was greater in piglets fed NC and ANT. In MLN, LAB count was greater in ANT and 30 mg IAP-fed piglets compared with 15 mg IAP. Piglets in 30 mg IAP in diet showed a tendency for lowering tissue necrosis compared with NC or ANT. Piglets fed 30 mg IAP showed a reduction in diarrhoea incidence in the pre- and post-challenge compared with 15 mg IAP and all other treatments, respectively. Based on the criteria, addition of 30 mg IAP to diet inhibits Enterobacteriaceae population and suggests a potential effect in mitigating intestinal injuries, as observed in piglets in the NC for some of the parameters investigated.
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spelling New findings of intestinal alkaline phosphatase: effects on intestinal and organ health of piglets challenged with ETEC F4 (K88)alkaline phosphatasebacterial populationsdiarrhoea incidenceintestinal histopathologyproinflammatory markerweanling pigsABSTRACT The aim of this study was to assess the addition of intestinal alkaline phosphatase (IAP) to diets on the count of bacterial populations, pH of digestive organ contents, histopathological description, proinflammatory markers, hepatic glycogen reserve, and diarrhoea incidence of piglets challenged with Escherichia coli. Sixty-four crossbred piglets (7.16±0.28 kg body weight, 25-days-old) were assigned to four treatments in a completely randomised block design: negative control (NC), NC + antibiotic (ANT), NC + 15 mg IAP, or NC + 30 mg IAP kg−1 of diet, eight replications of two piglets per experimental unit. All piglets were orally challenged with 6 mL of a solution containing enterotoxigenic Escherichia coli K88 at 106 CFU mL−1 at 15 days of experimentation. The study lasted for 19 days. At the end of the experimental period, the piglets were slaughtered (six animals per treatment). Enterobacteriaceae in caecum and colon was lower in piglets on 30 mg IAP than with ANT and NC, ANT or 15 mg IAP, respectively. Enterobacteriaceae adhered to the mesenteric lymph nodes (MLN) was greater in piglets fed ANT than the other treatments. Lactic acid bacteria (LAB) count in caecum was greater in piglets fed NC and ANT. In MLN, LAB count was greater in ANT and 30 mg IAP-fed piglets compared with 15 mg IAP. Piglets in 30 mg IAP in diet showed a tendency for lowering tissue necrosis compared with NC or ANT. Piglets fed 30 mg IAP showed a reduction in diarrhoea incidence in the pre- and post-challenge compared with 15 mg IAP and all other treatments, respectively. Based on the criteria, addition of 30 mg IAP to diet inhibits Enterobacteriaceae population and suggests a potential effect in mitigating intestinal injuries, as observed in piglets in the NC for some of the parameters investigated.Sociedade Brasileira de Zootecnia2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982022000100609Revista Brasileira de Zootecnia v.51 2022reponame:Revista Brasileira de Zootecnia (Online)instname:Sociedade Brasileira de Zootecnia (SBZ)instacron:SBZ10.37496/rbz5120210144info:eu-repo/semantics/openAccessGenova,Jansller LuizMelo,Antonio Diego BrandãoRupolo,Paulo EvaristoMacedo,Renata Ernlund Freitas deEngracia Filho,Jair RodiniCarvalho,Silvana TeixeiraFaucitano,LuigiCosta,Leandro BatistaCarvalho,Paulo Levi de Oliveiraeng2022-09-30T00:00:00Zoai:scielo:S1516-35982022000100609Revistahttps://www.rbz.org.br/pt-br/https://old.scielo.br/oai/scielo-oai.php||bz@sbz.org.br|| secretariarbz@sbz.org.br1806-92901516-3598opendoar:2022-09-30T00:00Revista Brasileira de Zootecnia (Online) - Sociedade Brasileira de Zootecnia (SBZ)false
dc.title.none.fl_str_mv New findings of intestinal alkaline phosphatase: effects on intestinal and organ health of piglets challenged with ETEC F4 (K88)
title New findings of intestinal alkaline phosphatase: effects on intestinal and organ health of piglets challenged with ETEC F4 (K88)
spellingShingle New findings of intestinal alkaline phosphatase: effects on intestinal and organ health of piglets challenged with ETEC F4 (K88)
Genova,Jansller Luiz
alkaline phosphatase
bacterial populations
diarrhoea incidence
intestinal histopathology
proinflammatory marker
weanling pigs
title_short New findings of intestinal alkaline phosphatase: effects on intestinal and organ health of piglets challenged with ETEC F4 (K88)
title_full New findings of intestinal alkaline phosphatase: effects on intestinal and organ health of piglets challenged with ETEC F4 (K88)
title_fullStr New findings of intestinal alkaline phosphatase: effects on intestinal and organ health of piglets challenged with ETEC F4 (K88)
title_full_unstemmed New findings of intestinal alkaline phosphatase: effects on intestinal and organ health of piglets challenged with ETEC F4 (K88)
title_sort New findings of intestinal alkaline phosphatase: effects on intestinal and organ health of piglets challenged with ETEC F4 (K88)
author Genova,Jansller Luiz
author_facet Genova,Jansller Luiz
Melo,Antonio Diego Brandão
Rupolo,Paulo Evaristo
Macedo,Renata Ernlund Freitas de
Engracia Filho,Jair Rodini
Carvalho,Silvana Teixeira
Faucitano,Luigi
Costa,Leandro Batista
Carvalho,Paulo Levi de Oliveira
author_role author
author2 Melo,Antonio Diego Brandão
Rupolo,Paulo Evaristo
Macedo,Renata Ernlund Freitas de
Engracia Filho,Jair Rodini
Carvalho,Silvana Teixeira
Faucitano,Luigi
Costa,Leandro Batista
Carvalho,Paulo Levi de Oliveira
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Genova,Jansller Luiz
Melo,Antonio Diego Brandão
Rupolo,Paulo Evaristo
Macedo,Renata Ernlund Freitas de
Engracia Filho,Jair Rodini
Carvalho,Silvana Teixeira
Faucitano,Luigi
Costa,Leandro Batista
Carvalho,Paulo Levi de Oliveira
dc.subject.por.fl_str_mv alkaline phosphatase
bacterial populations
diarrhoea incidence
intestinal histopathology
proinflammatory marker
weanling pigs
topic alkaline phosphatase
bacterial populations
diarrhoea incidence
intestinal histopathology
proinflammatory marker
weanling pigs
description ABSTRACT The aim of this study was to assess the addition of intestinal alkaline phosphatase (IAP) to diets on the count of bacterial populations, pH of digestive organ contents, histopathological description, proinflammatory markers, hepatic glycogen reserve, and diarrhoea incidence of piglets challenged with Escherichia coli. Sixty-four crossbred piglets (7.16±0.28 kg body weight, 25-days-old) were assigned to four treatments in a completely randomised block design: negative control (NC), NC + antibiotic (ANT), NC + 15 mg IAP, or NC + 30 mg IAP kg−1 of diet, eight replications of two piglets per experimental unit. All piglets were orally challenged with 6 mL of a solution containing enterotoxigenic Escherichia coli K88 at 106 CFU mL−1 at 15 days of experimentation. The study lasted for 19 days. At the end of the experimental period, the piglets were slaughtered (six animals per treatment). Enterobacteriaceae in caecum and colon was lower in piglets on 30 mg IAP than with ANT and NC, ANT or 15 mg IAP, respectively. Enterobacteriaceae adhered to the mesenteric lymph nodes (MLN) was greater in piglets fed ANT than the other treatments. Lactic acid bacteria (LAB) count in caecum was greater in piglets fed NC and ANT. In MLN, LAB count was greater in ANT and 30 mg IAP-fed piglets compared with 15 mg IAP. Piglets in 30 mg IAP in diet showed a tendency for lowering tissue necrosis compared with NC or ANT. Piglets fed 30 mg IAP showed a reduction in diarrhoea incidence in the pre- and post-challenge compared with 15 mg IAP and all other treatments, respectively. Based on the criteria, addition of 30 mg IAP to diet inhibits Enterobacteriaceae population and suggests a potential effect in mitigating intestinal injuries, as observed in piglets in the NC for some of the parameters investigated.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982022000100609
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982022000100609
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.37496/rbz5120210144
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Zootecnia
publisher.none.fl_str_mv Sociedade Brasileira de Zootecnia
dc.source.none.fl_str_mv Revista Brasileira de Zootecnia v.51 2022
reponame:Revista Brasileira de Zootecnia (Online)
instname:Sociedade Brasileira de Zootecnia (SBZ)
instacron:SBZ
instname_str Sociedade Brasileira de Zootecnia (SBZ)
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institution SBZ
reponame_str Revista Brasileira de Zootecnia (Online)
collection Revista Brasileira de Zootecnia (Online)
repository.name.fl_str_mv Revista Brasileira de Zootecnia (Online) - Sociedade Brasileira de Zootecnia (SBZ)
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