Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol

Detalhes bibliográficos
Autor(a) principal: Machado,Gilmara Junqueira
Data de Publicação: 2019
Outros Autores: Murgas,Luis David Solis, Palhares,Priscila Cotta, Assis,Isadora de Lima, Castro,Tássia Flávia Dias
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Zootecnia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100155
Resumo: ABSTRACT This study aimed to evaluate the effect of using different concentrations (5, 7.5, 10, and 12.5%) of two permeating cryoprotectants, dimethyl sulphoxide (DMSO) and methanol, on Prochilodus lineatus embryos while being subjected to cooling for 2, 4, 6, and 8 h at 4 °C. We analyzed the hatching rate, viable larvae, and counts of hatched and spoiled eggs and those that did not complete their development after cooling. We observed a significant interaction among variables, permeable cryoprotectant concentration, and cooling time, having the increase of these factors caused a reduction in hatching rate. The curimba embryos showed a higher sensitivity to cold at a temperature of 4 °C for 6 and 8 h, directly influencing the production of viable larvae. We did not observe a significant difference in the variables analyzed when comparing the cryoprotectant solutions containing DMSO, methanol, and solutions containing only water and sucrose (0.5M). An increase in the concentration of cryoprotectant and cooling time promotes a reduction in the hatching rate and in the percentage of viable curimba larvae.
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spelling Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanolaquaculturebiotechnologycryopreservationeggsfishlarvaeABSTRACT This study aimed to evaluate the effect of using different concentrations (5, 7.5, 10, and 12.5%) of two permeating cryoprotectants, dimethyl sulphoxide (DMSO) and methanol, on Prochilodus lineatus embryos while being subjected to cooling for 2, 4, 6, and 8 h at 4 °C. We analyzed the hatching rate, viable larvae, and counts of hatched and spoiled eggs and those that did not complete their development after cooling. We observed a significant interaction among variables, permeable cryoprotectant concentration, and cooling time, having the increase of these factors caused a reduction in hatching rate. The curimba embryos showed a higher sensitivity to cold at a temperature of 4 °C for 6 and 8 h, directly influencing the production of viable larvae. We did not observe a significant difference in the variables analyzed when comparing the cryoprotectant solutions containing DMSO, methanol, and solutions containing only water and sucrose (0.5M). An increase in the concentration of cryoprotectant and cooling time promotes a reduction in the hatching rate and in the percentage of viable curimba larvae.Sociedade Brasileira de Zootecnia2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100155Revista Brasileira de Zootecnia v.48 2019reponame:Revista Brasileira de Zootecnia (Online)instname:Sociedade Brasileira de Zootecnia (SBZ)instacron:SBZ10.1590/rbz4820160377info:eu-repo/semantics/openAccessMachado,Gilmara JunqueiraMurgas,Luis David SolisPalhares,Priscila CottaAssis,Isadora de LimaCastro,Tássia Flávia Diaseng2019-07-17T00:00:00Zoai:scielo:S1516-35982019000100155Revistahttps://www.rbz.org.br/pt-br/https://old.scielo.br/oai/scielo-oai.php||bz@sbz.org.br|| secretariarbz@sbz.org.br1806-92901516-3598opendoar:2019-07-17T00:00Revista Brasileira de Zootecnia (Online) - Sociedade Brasileira de Zootecnia (SBZ)false
dc.title.none.fl_str_mv Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol
title Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol
spellingShingle Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol
Machado,Gilmara Junqueira
aquaculture
biotechnology
cryopreservation
eggs
fish
larvae
title_short Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol
title_full Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol
title_fullStr Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol
title_full_unstemmed Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol
title_sort Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol
author Machado,Gilmara Junqueira
author_facet Machado,Gilmara Junqueira
Murgas,Luis David Solis
Palhares,Priscila Cotta
Assis,Isadora de Lima
Castro,Tássia Flávia Dias
author_role author
author2 Murgas,Luis David Solis
Palhares,Priscila Cotta
Assis,Isadora de Lima
Castro,Tássia Flávia Dias
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Machado,Gilmara Junqueira
Murgas,Luis David Solis
Palhares,Priscila Cotta
Assis,Isadora de Lima
Castro,Tássia Flávia Dias
dc.subject.por.fl_str_mv aquaculture
biotechnology
cryopreservation
eggs
fish
larvae
topic aquaculture
biotechnology
cryopreservation
eggs
fish
larvae
description ABSTRACT This study aimed to evaluate the effect of using different concentrations (5, 7.5, 10, and 12.5%) of two permeating cryoprotectants, dimethyl sulphoxide (DMSO) and methanol, on Prochilodus lineatus embryos while being subjected to cooling for 2, 4, 6, and 8 h at 4 °C. We analyzed the hatching rate, viable larvae, and counts of hatched and spoiled eggs and those that did not complete their development after cooling. We observed a significant interaction among variables, permeable cryoprotectant concentration, and cooling time, having the increase of these factors caused a reduction in hatching rate. The curimba embryos showed a higher sensitivity to cold at a temperature of 4 °C for 6 and 8 h, directly influencing the production of viable larvae. We did not observe a significant difference in the variables analyzed when comparing the cryoprotectant solutions containing DMSO, methanol, and solutions containing only water and sucrose (0.5M). An increase in the concentration of cryoprotectant and cooling time promotes a reduction in the hatching rate and in the percentage of viable curimba larvae.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100155
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dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv 10.1590/rbz4820160377
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Zootecnia
publisher.none.fl_str_mv Sociedade Brasileira de Zootecnia
dc.source.none.fl_str_mv Revista Brasileira de Zootecnia v.48 2019
reponame:Revista Brasileira de Zootecnia (Online)
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