Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Zootecnia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100155 |
Resumo: | ABSTRACT This study aimed to evaluate the effect of using different concentrations (5, 7.5, 10, and 12.5%) of two permeating cryoprotectants, dimethyl sulphoxide (DMSO) and methanol, on Prochilodus lineatus embryos while being subjected to cooling for 2, 4, 6, and 8 h at 4 °C. We analyzed the hatching rate, viable larvae, and counts of hatched and spoiled eggs and those that did not complete their development after cooling. We observed a significant interaction among variables, permeable cryoprotectant concentration, and cooling time, having the increase of these factors caused a reduction in hatching rate. The curimba embryos showed a higher sensitivity to cold at a temperature of 4 °C for 6 and 8 h, directly influencing the production of viable larvae. We did not observe a significant difference in the variables analyzed when comparing the cryoprotectant solutions containing DMSO, methanol, and solutions containing only water and sucrose (0.5M). An increase in the concentration of cryoprotectant and cooling time promotes a reduction in the hatching rate and in the percentage of viable curimba larvae. |
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Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanolaquaculturebiotechnologycryopreservationeggsfishlarvaeABSTRACT This study aimed to evaluate the effect of using different concentrations (5, 7.5, 10, and 12.5%) of two permeating cryoprotectants, dimethyl sulphoxide (DMSO) and methanol, on Prochilodus lineatus embryos while being subjected to cooling for 2, 4, 6, and 8 h at 4 °C. We analyzed the hatching rate, viable larvae, and counts of hatched and spoiled eggs and those that did not complete their development after cooling. We observed a significant interaction among variables, permeable cryoprotectant concentration, and cooling time, having the increase of these factors caused a reduction in hatching rate. The curimba embryos showed a higher sensitivity to cold at a temperature of 4 °C for 6 and 8 h, directly influencing the production of viable larvae. We did not observe a significant difference in the variables analyzed when comparing the cryoprotectant solutions containing DMSO, methanol, and solutions containing only water and sucrose (0.5M). An increase in the concentration of cryoprotectant and cooling time promotes a reduction in the hatching rate and in the percentage of viable curimba larvae.Sociedade Brasileira de Zootecnia2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100155Revista Brasileira de Zootecnia v.48 2019reponame:Revista Brasileira de Zootecnia (Online)instname:Sociedade Brasileira de Zootecnia (SBZ)instacron:SBZ10.1590/rbz4820160377info:eu-repo/semantics/openAccessMachado,Gilmara JunqueiraMurgas,Luis David SolisPalhares,Priscila CottaAssis,Isadora de LimaCastro,Tássia Flávia Diaseng2019-07-17T00:00:00Zoai:scielo:S1516-35982019000100155Revistahttps://www.rbz.org.br/pt-br/https://old.scielo.br/oai/scielo-oai.php||bz@sbz.org.br|| secretariarbz@sbz.org.br1806-92901516-3598opendoar:2019-07-17T00:00Revista Brasileira de Zootecnia (Online) - Sociedade Brasileira de Zootecnia (SBZ)false |
dc.title.none.fl_str_mv |
Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol |
title |
Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol |
spellingShingle |
Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol Machado,Gilmara Junqueira aquaculture biotechnology cryopreservation eggs fish larvae |
title_short |
Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol |
title_full |
Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol |
title_fullStr |
Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol |
title_full_unstemmed |
Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol |
title_sort |
Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol |
author |
Machado,Gilmara Junqueira |
author_facet |
Machado,Gilmara Junqueira Murgas,Luis David Solis Palhares,Priscila Cotta Assis,Isadora de Lima Castro,Tássia Flávia Dias |
author_role |
author |
author2 |
Murgas,Luis David Solis Palhares,Priscila Cotta Assis,Isadora de Lima Castro,Tássia Flávia Dias |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Machado,Gilmara Junqueira Murgas,Luis David Solis Palhares,Priscila Cotta Assis,Isadora de Lima Castro,Tássia Flávia Dias |
dc.subject.por.fl_str_mv |
aquaculture biotechnology cryopreservation eggs fish larvae |
topic |
aquaculture biotechnology cryopreservation eggs fish larvae |
description |
ABSTRACT This study aimed to evaluate the effect of using different concentrations (5, 7.5, 10, and 12.5%) of two permeating cryoprotectants, dimethyl sulphoxide (DMSO) and methanol, on Prochilodus lineatus embryos while being subjected to cooling for 2, 4, 6, and 8 h at 4 °C. We analyzed the hatching rate, viable larvae, and counts of hatched and spoiled eggs and those that did not complete their development after cooling. We observed a significant interaction among variables, permeable cryoprotectant concentration, and cooling time, having the increase of these factors caused a reduction in hatching rate. The curimba embryos showed a higher sensitivity to cold at a temperature of 4 °C for 6 and 8 h, directly influencing the production of viable larvae. We did not observe a significant difference in the variables analyzed when comparing the cryoprotectant solutions containing DMSO, methanol, and solutions containing only water and sucrose (0.5M). An increase in the concentration of cryoprotectant and cooling time promotes a reduction in the hatching rate and in the percentage of viable curimba larvae. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100155 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100155 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/rbz4820160377 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Zootecnia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Zootecnia |
dc.source.none.fl_str_mv |
Revista Brasileira de Zootecnia v.48 2019 reponame:Revista Brasileira de Zootecnia (Online) instname:Sociedade Brasileira de Zootecnia (SBZ) instacron:SBZ |
instname_str |
Sociedade Brasileira de Zootecnia (SBZ) |
instacron_str |
SBZ |
institution |
SBZ |
reponame_str |
Revista Brasileira de Zootecnia (Online) |
collection |
Revista Brasileira de Zootecnia (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Zootecnia (Online) - Sociedade Brasileira de Zootecnia (SBZ) |
repository.mail.fl_str_mv |
||bz@sbz.org.br|| secretariarbz@sbz.org.br |
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1750318153195323392 |