Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Zootecnia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000300003 |
Resumo: | This study evaluated the influence of follicular fluid (FF) added to the maturation medium on the quality of bovine embryos produced in vitro. In the first experiment, oocytes were matured in media containing five different FF concentrations with different maturation times and classified according to meiotic progression and migration of cortical granules. In the second experiment, oocytes matured in the same media were fertilized at three different maturation times; thereafter, cleavage and blastocyst rates were evaluated. In the third experiment, oocytes were matured in media containing three different FF concentrations at two different maturation times, and embryo quality, inferred by the ratio of inner cell mass and trophectoderm cells compared with total cell number, was evaluated. Higher FF concentration (75 - 100% FF) slowed meiotic progression and CG migration (control - 78.13% vs. treated - 52.58% and control - 52.7% vs. treated - 11.59%, respectively, at 24 h of maturation). Also, FF at concentration of 75% or 100% had a negative influence on cleavage and blastocyst rates (control - 90.13% vs. treated - 82.64% and control - 35.73% vs. treated - 11.57%, respectively, at 24 h of maturation). The 50% FF resulted in embryos with increased inner cell mass numbers (control - 29.91 vs. treated - 35.49, at 24 h of maturation) and total cell numbers (control - 109.53 vs. treated - 120.67, at 26 h of maturation). Even though higher concentration of FF added to the maturation medium reduced embryonic development rates, in lower concentrations, FF slowed the meiotic progression and migration of CG and contributed to increases in inner cell mass number. Thus, FF added to the maturation medium enhances the number of cells in bovine embryos produced in vitro, especially for inner cell mass. |
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Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitroembryo developmentinner cell massin vitro fertilizationoocytesThis study evaluated the influence of follicular fluid (FF) added to the maturation medium on the quality of bovine embryos produced in vitro. In the first experiment, oocytes were matured in media containing five different FF concentrations with different maturation times and classified according to meiotic progression and migration of cortical granules. In the second experiment, oocytes matured in the same media were fertilized at three different maturation times; thereafter, cleavage and blastocyst rates were evaluated. In the third experiment, oocytes were matured in media containing three different FF concentrations at two different maturation times, and embryo quality, inferred by the ratio of inner cell mass and trophectoderm cells compared with total cell number, was evaluated. Higher FF concentration (75 - 100% FF) slowed meiotic progression and CG migration (control - 78.13% vs. treated - 52.58% and control - 52.7% vs. treated - 11.59%, respectively, at 24 h of maturation). Also, FF at concentration of 75% or 100% had a negative influence on cleavage and blastocyst rates (control - 90.13% vs. treated - 82.64% and control - 35.73% vs. treated - 11.57%, respectively, at 24 h of maturation). The 50% FF resulted in embryos with increased inner cell mass numbers (control - 29.91 vs. treated - 35.49, at 24 h of maturation) and total cell numbers (control - 109.53 vs. treated - 120.67, at 26 h of maturation). Even though higher concentration of FF added to the maturation medium reduced embryonic development rates, in lower concentrations, FF slowed the meiotic progression and migration of CG and contributed to increases in inner cell mass number. Thus, FF added to the maturation medium enhances the number of cells in bovine embryos produced in vitro, especially for inner cell mass.Sociedade Brasileira de Zootecnia2014-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000300003Revista Brasileira de Zootecnia v.43 n.3 2014reponame:Revista Brasileira de Zootecnia (Online)instname:Sociedade Brasileira de Zootecnia (SBZ)instacron:SBZ10.1590/S1516-35982014000300003info:eu-repo/semantics/openAccessCruz,Maria Helena CoelhoSaraiva,Naiara ZoccalCruz,Jurandir Ferreira daOliveira,Clara SladeDel Collado,MaiteFernandes,HugoCastro,Fernanda Cavallari deGarcia,Joaquim Mansanoeng2014-03-11T00:00:00Zoai:scielo:S1516-35982014000300003Revistahttps://www.rbz.org.br/pt-br/https://old.scielo.br/oai/scielo-oai.php||bz@sbz.org.br|| secretariarbz@sbz.org.br1806-92901516-3598opendoar:2014-03-11T00:00Revista Brasileira de Zootecnia (Online) - Sociedade Brasileira de Zootecnia (SBZ)false |
dc.title.none.fl_str_mv |
Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro |
title |
Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro |
spellingShingle |
Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro Cruz,Maria Helena Coelho embryo development inner cell mass in vitro fertilization oocytes |
title_short |
Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro |
title_full |
Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro |
title_fullStr |
Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro |
title_full_unstemmed |
Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro |
title_sort |
Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro |
author |
Cruz,Maria Helena Coelho |
author_facet |
Cruz,Maria Helena Coelho Saraiva,Naiara Zoccal Cruz,Jurandir Ferreira da Oliveira,Clara Slade Del Collado,Maite Fernandes,Hugo Castro,Fernanda Cavallari de Garcia,Joaquim Mansano |
author_role |
author |
author2 |
Saraiva,Naiara Zoccal Cruz,Jurandir Ferreira da Oliveira,Clara Slade Del Collado,Maite Fernandes,Hugo Castro,Fernanda Cavallari de Garcia,Joaquim Mansano |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Cruz,Maria Helena Coelho Saraiva,Naiara Zoccal Cruz,Jurandir Ferreira da Oliveira,Clara Slade Del Collado,Maite Fernandes,Hugo Castro,Fernanda Cavallari de Garcia,Joaquim Mansano |
dc.subject.por.fl_str_mv |
embryo development inner cell mass in vitro fertilization oocytes |
topic |
embryo development inner cell mass in vitro fertilization oocytes |
description |
This study evaluated the influence of follicular fluid (FF) added to the maturation medium on the quality of bovine embryos produced in vitro. In the first experiment, oocytes were matured in media containing five different FF concentrations with different maturation times and classified according to meiotic progression and migration of cortical granules. In the second experiment, oocytes matured in the same media were fertilized at three different maturation times; thereafter, cleavage and blastocyst rates were evaluated. In the third experiment, oocytes were matured in media containing three different FF concentrations at two different maturation times, and embryo quality, inferred by the ratio of inner cell mass and trophectoderm cells compared with total cell number, was evaluated. Higher FF concentration (75 - 100% FF) slowed meiotic progression and CG migration (control - 78.13% vs. treated - 52.58% and control - 52.7% vs. treated - 11.59%, respectively, at 24 h of maturation). Also, FF at concentration of 75% or 100% had a negative influence on cleavage and blastocyst rates (control - 90.13% vs. treated - 82.64% and control - 35.73% vs. treated - 11.57%, respectively, at 24 h of maturation). The 50% FF resulted in embryos with increased inner cell mass numbers (control - 29.91 vs. treated - 35.49, at 24 h of maturation) and total cell numbers (control - 109.53 vs. treated - 120.67, at 26 h of maturation). Even though higher concentration of FF added to the maturation medium reduced embryonic development rates, in lower concentrations, FF slowed the meiotic progression and migration of CG and contributed to increases in inner cell mass number. Thus, FF added to the maturation medium enhances the number of cells in bovine embryos produced in vitro, especially for inner cell mass. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000300003 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000300003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-35982014000300003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Zootecnia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Zootecnia |
dc.source.none.fl_str_mv |
Revista Brasileira de Zootecnia v.43 n.3 2014 reponame:Revista Brasileira de Zootecnia (Online) instname:Sociedade Brasileira de Zootecnia (SBZ) instacron:SBZ |
instname_str |
Sociedade Brasileira de Zootecnia (SBZ) |
instacron_str |
SBZ |
institution |
SBZ |
reponame_str |
Revista Brasileira de Zootecnia (Online) |
collection |
Revista Brasileira de Zootecnia (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Zootecnia (Online) - Sociedade Brasileira de Zootecnia (SBZ) |
repository.mail.fl_str_mv |
||bz@sbz.org.br|| secretariarbz@sbz.org.br |
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1750318150980730880 |