Hemolytic activity of alternative complement pathway as an indicator of innate immunity in pacu (Piaractus mesopotamicus)

Detalhes bibliográficos
Autor(a) principal: Biller-Takahashi,Jaqueline Dalbello
Data de Publicação: 2012
Outros Autores: Takahashi,Leonardo Susumu, Marzocchi-Machado,Cleni Mara, Zanuzzo,Fabio Sabbadin, Sabioni,Rafael Estevan, Urbinati,Elisabeth Criscuolo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Zootecnia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000200001
Resumo: The objective of this study was to evaluate the methodology to establish the hemolytic activity of alternative complement pathway as an indicator of the innate immunity in Brazilian fish pacu (Piaractus mesopotamicus), in addition to verifying the influence of β-glucan as an immunostimulant. Fish were fed with diets containing 0, 0.1 and 1% β-glucan, during seven days, and then inoculated with Aeromonas hydrophila. Seven days after the challenge, they were bled for serum extraction. The methodology consisted of a kinetic assay that allows calculating the required time for serum proteins of the complement to promote 50% lysis of a rabbit red blood cell suspension. The method developed in mammals was successfully applied for pacu and determined that the hemolytic activity of the proteins of the complement system (alternative pathway) increased after the pathogen challenge, but was not influenced by the β-glucan treatment.
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spelling Hemolytic activity of alternative complement pathway as an indicator of innate immunity in pacu (Piaractus mesopotamicus)A. hydrophilaβ-glucanimmune responsepacuThe objective of this study was to evaluate the methodology to establish the hemolytic activity of alternative complement pathway as an indicator of the innate immunity in Brazilian fish pacu (Piaractus mesopotamicus), in addition to verifying the influence of β-glucan as an immunostimulant. Fish were fed with diets containing 0, 0.1 and 1% β-glucan, during seven days, and then inoculated with Aeromonas hydrophila. Seven days after the challenge, they were bled for serum extraction. The methodology consisted of a kinetic assay that allows calculating the required time for serum proteins of the complement to promote 50% lysis of a rabbit red blood cell suspension. The method developed in mammals was successfully applied for pacu and determined that the hemolytic activity of the proteins of the complement system (alternative pathway) increased after the pathogen challenge, but was not influenced by the β-glucan treatment.Sociedade Brasileira de Zootecnia2012-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000200001Revista Brasileira de Zootecnia v.41 n.2 2012reponame:Revista Brasileira de Zootecnia (Online)instname:Sociedade Brasileira de Zootecnia (SBZ)instacron:SBZ10.1590/S1516-35982012000200001info:eu-repo/semantics/openAccessBiller-Takahashi,Jaqueline DalbelloTakahashi,Leonardo SusumuMarzocchi-Machado,Cleni MaraZanuzzo,Fabio SabbadinSabioni,Rafael EstevanUrbinati,Elisabeth Criscuoloeng2012-03-19T00:00:00Zoai:scielo:S1516-35982012000200001Revistahttps://www.rbz.org.br/pt-br/https://old.scielo.br/oai/scielo-oai.php||bz@sbz.org.br|| secretariarbz@sbz.org.br1806-92901516-3598opendoar:2012-03-19T00:00Revista Brasileira de Zootecnia (Online) - Sociedade Brasileira de Zootecnia (SBZ)false
dc.title.none.fl_str_mv Hemolytic activity of alternative complement pathway as an indicator of innate immunity in pacu (Piaractus mesopotamicus)
title Hemolytic activity of alternative complement pathway as an indicator of innate immunity in pacu (Piaractus mesopotamicus)
spellingShingle Hemolytic activity of alternative complement pathway as an indicator of innate immunity in pacu (Piaractus mesopotamicus)
Biller-Takahashi,Jaqueline Dalbello
A. hydrophila
β-glucan
immune response
pacu
title_short Hemolytic activity of alternative complement pathway as an indicator of innate immunity in pacu (Piaractus mesopotamicus)
title_full Hemolytic activity of alternative complement pathway as an indicator of innate immunity in pacu (Piaractus mesopotamicus)
title_fullStr Hemolytic activity of alternative complement pathway as an indicator of innate immunity in pacu (Piaractus mesopotamicus)
title_full_unstemmed Hemolytic activity of alternative complement pathway as an indicator of innate immunity in pacu (Piaractus mesopotamicus)
title_sort Hemolytic activity of alternative complement pathway as an indicator of innate immunity in pacu (Piaractus mesopotamicus)
author Biller-Takahashi,Jaqueline Dalbello
author_facet Biller-Takahashi,Jaqueline Dalbello
Takahashi,Leonardo Susumu
Marzocchi-Machado,Cleni Mara
Zanuzzo,Fabio Sabbadin
Sabioni,Rafael Estevan
Urbinati,Elisabeth Criscuolo
author_role author
author2 Takahashi,Leonardo Susumu
Marzocchi-Machado,Cleni Mara
Zanuzzo,Fabio Sabbadin
Sabioni,Rafael Estevan
Urbinati,Elisabeth Criscuolo
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Biller-Takahashi,Jaqueline Dalbello
Takahashi,Leonardo Susumu
Marzocchi-Machado,Cleni Mara
Zanuzzo,Fabio Sabbadin
Sabioni,Rafael Estevan
Urbinati,Elisabeth Criscuolo
dc.subject.por.fl_str_mv A. hydrophila
β-glucan
immune response
pacu
topic A. hydrophila
β-glucan
immune response
pacu
description The objective of this study was to evaluate the methodology to establish the hemolytic activity of alternative complement pathway as an indicator of the innate immunity in Brazilian fish pacu (Piaractus mesopotamicus), in addition to verifying the influence of β-glucan as an immunostimulant. Fish were fed with diets containing 0, 0.1 and 1% β-glucan, during seven days, and then inoculated with Aeromonas hydrophila. Seven days after the challenge, they were bled for serum extraction. The methodology consisted of a kinetic assay that allows calculating the required time for serum proteins of the complement to promote 50% lysis of a rabbit red blood cell suspension. The method developed in mammals was successfully applied for pacu and determined that the hemolytic activity of the proteins of the complement system (alternative pathway) increased after the pathogen challenge, but was not influenced by the β-glucan treatment.
publishDate 2012
dc.date.none.fl_str_mv 2012-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000200001
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000200001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-35982012000200001
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Zootecnia
publisher.none.fl_str_mv Sociedade Brasileira de Zootecnia
dc.source.none.fl_str_mv Revista Brasileira de Zootecnia v.41 n.2 2012
reponame:Revista Brasileira de Zootecnia (Online)
instname:Sociedade Brasileira de Zootecnia (SBZ)
instacron:SBZ
instname_str Sociedade Brasileira de Zootecnia (SBZ)
instacron_str SBZ
institution SBZ
reponame_str Revista Brasileira de Zootecnia (Online)
collection Revista Brasileira de Zootecnia (Online)
repository.name.fl_str_mv Revista Brasileira de Zootecnia (Online) - Sociedade Brasileira de Zootecnia (SBZ)
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