A molecular method to detect and identify the native species of southwestern Atlantic Crassostrea (Mollusca: Ostreidae)
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Zoologia (Curitiba. Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702011000400002 |
Resumo: | Among oysters, species of Crassostrea (Sacco, 1897) are the most attractive to aquaculture. In Brazil, the genus is represented by C. rhizophorae (Guilding, 1828) and C. brasiliana (Lamarck, 1819). Because the maturation and breeding technology is not well developed for these species, aquaculturists need a reliable method to decide the correct time to place spat collectors in the field, and to identify both species, which are morphologically similar. In this study a specific Multiplex PCR protocol was developed, using one pair of universal primers from 18S rDNA as a positive control and a pair of specific primers for each target species. The sensitivity and specificity of the protocol was evaluated. It detected C. rhizophorae DNA in low concentrations, and C. brasiliana DNA in even lower concentrations. Further, the Multiplex PCR proved efficient in detecting DNA in concentrations equivalent to that of a single larva of each species, either separated or combined, when mixed with total DNA extract of a plankton sample representing 1000 L of filtered water. Field tests confirmed the applicability of the protocol, which holds the promise to become an important tool for aquaculture or conservation programs, allowing for the continuous monitoring of the life cycle of C. brasiliana and C. rhizophorae, by detecting the right periods of larval release and settlement. |
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A molecular method to detect and identify the native species of southwestern Atlantic Crassostrea (Mollusca: Ostreidae)Multiplex PCRoystersplanktonspecific primersAmong oysters, species of Crassostrea (Sacco, 1897) are the most attractive to aquaculture. In Brazil, the genus is represented by C. rhizophorae (Guilding, 1828) and C. brasiliana (Lamarck, 1819). Because the maturation and breeding technology is not well developed for these species, aquaculturists need a reliable method to decide the correct time to place spat collectors in the field, and to identify both species, which are morphologically similar. In this study a specific Multiplex PCR protocol was developed, using one pair of universal primers from 18S rDNA as a positive control and a pair of specific primers for each target species. The sensitivity and specificity of the protocol was evaluated. It detected C. rhizophorae DNA in low concentrations, and C. brasiliana DNA in even lower concentrations. Further, the Multiplex PCR proved efficient in detecting DNA in concentrations equivalent to that of a single larva of each species, either separated or combined, when mixed with total DNA extract of a plankton sample representing 1000 L of filtered water. Field tests confirmed the applicability of the protocol, which holds the promise to become an important tool for aquaculture or conservation programs, allowing for the continuous monitoring of the life cycle of C. brasiliana and C. rhizophorae, by detecting the right periods of larval release and settlement.Sociedade Brasileira de Zoologia2011-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702011000400002Zoologia (Curitiba) v.28 n.4 2011reponame:Zoologia (Curitiba. Online)instname:Sociedade Brasileira de Zoologiainstacron:SBZ10.1590/S1984-46702011000400002info:eu-repo/semantics/openAccessLudwig,SandraPatella,RaquelStoiev,SérgioCastilho-Westphal,GiselaGirotto,Marcus V. FOstrensky,Antonioeng2011-09-16T00:00:00Zoai:scielo:S1984-46702011000400002Revistahttp://www.scielo.br/zoolONGhttps://old.scielo.br/oai/scielo-oai.phpsbz@sbzoologia.org.br1984-46891984-4670opendoar:2011-09-16T00:00Zoologia (Curitiba. Online) - Sociedade Brasileira de Zoologiafalse |
dc.title.none.fl_str_mv |
A molecular method to detect and identify the native species of southwestern Atlantic Crassostrea (Mollusca: Ostreidae) |
title |
A molecular method to detect and identify the native species of southwestern Atlantic Crassostrea (Mollusca: Ostreidae) |
spellingShingle |
A molecular method to detect and identify the native species of southwestern Atlantic Crassostrea (Mollusca: Ostreidae) Ludwig,Sandra Multiplex PCR oysters plankton specific primers |
title_short |
A molecular method to detect and identify the native species of southwestern Atlantic Crassostrea (Mollusca: Ostreidae) |
title_full |
A molecular method to detect and identify the native species of southwestern Atlantic Crassostrea (Mollusca: Ostreidae) |
title_fullStr |
A molecular method to detect and identify the native species of southwestern Atlantic Crassostrea (Mollusca: Ostreidae) |
title_full_unstemmed |
A molecular method to detect and identify the native species of southwestern Atlantic Crassostrea (Mollusca: Ostreidae) |
title_sort |
A molecular method to detect and identify the native species of southwestern Atlantic Crassostrea (Mollusca: Ostreidae) |
author |
Ludwig,Sandra |
author_facet |
Ludwig,Sandra Patella,Raquel Stoiev,Sérgio Castilho-Westphal,Gisela Girotto,Marcus V. F Ostrensky,Antonio |
author_role |
author |
author2 |
Patella,Raquel Stoiev,Sérgio Castilho-Westphal,Gisela Girotto,Marcus V. F Ostrensky,Antonio |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Ludwig,Sandra Patella,Raquel Stoiev,Sérgio Castilho-Westphal,Gisela Girotto,Marcus V. F Ostrensky,Antonio |
dc.subject.por.fl_str_mv |
Multiplex PCR oysters plankton specific primers |
topic |
Multiplex PCR oysters plankton specific primers |
description |
Among oysters, species of Crassostrea (Sacco, 1897) are the most attractive to aquaculture. In Brazil, the genus is represented by C. rhizophorae (Guilding, 1828) and C. brasiliana (Lamarck, 1819). Because the maturation and breeding technology is not well developed for these species, aquaculturists need a reliable method to decide the correct time to place spat collectors in the field, and to identify both species, which are morphologically similar. In this study a specific Multiplex PCR protocol was developed, using one pair of universal primers from 18S rDNA as a positive control and a pair of specific primers for each target species. The sensitivity and specificity of the protocol was evaluated. It detected C. rhizophorae DNA in low concentrations, and C. brasiliana DNA in even lower concentrations. Further, the Multiplex PCR proved efficient in detecting DNA in concentrations equivalent to that of a single larva of each species, either separated or combined, when mixed with total DNA extract of a plankton sample representing 1000 L of filtered water. Field tests confirmed the applicability of the protocol, which holds the promise to become an important tool for aquaculture or conservation programs, allowing for the continuous monitoring of the life cycle of C. brasiliana and C. rhizophorae, by detecting the right periods of larval release and settlement. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702011000400002 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702011000400002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1984-46702011000400002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Zoologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Zoologia |
dc.source.none.fl_str_mv |
Zoologia (Curitiba) v.28 n.4 2011 reponame:Zoologia (Curitiba. Online) instname:Sociedade Brasileira de Zoologia instacron:SBZ |
instname_str |
Sociedade Brasileira de Zoologia |
instacron_str |
SBZ |
institution |
SBZ |
reponame_str |
Zoologia (Curitiba. Online) |
collection |
Zoologia (Curitiba. Online) |
repository.name.fl_str_mv |
Zoologia (Curitiba. Online) - Sociedade Brasileira de Zoologia |
repository.mail.fl_str_mv |
sbz@sbzoologia.org.br |
_version_ |
1750318090513547264 |