Evaluation of lugol solution used for counting rumen ciliates

Detalhes bibliográficos
Autor(a) principal: D'Agosto,Marta
Data de Publicação: 1999
Outros Autores: Carneiro,Maria Elisa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Zoologia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751999000300011
Resumo: The present study proposes a modification of the technique described by PURSER & MOIR (1959) for the quantitative evaluation of rumen ciliate based on an adaptation described by DEHORITY (I984). The modifying process includes: the replacement of two drops of brilliant green dye, for at least four hours, by three drops of lugol solution, for at least 15 minutes. It was made a comparative evaluation of these stainings. It was concluded that lugol solution can replace the brilliant green dye showing the following advantages: staining time reduction and subsequent speeding of sample processing; evidence of skeletal plates of entodiniomorphs making its identification easier; improved observation of small ciliates and inconspicuous structures; improved total counting and generic identification of the ciliates.
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spelling Evaluation of lugol solution used for counting rumen ciliatesRumen ciliatequantitative evaluationlugol solutiontechniqueThe present study proposes a modification of the technique described by PURSER & MOIR (1959) for the quantitative evaluation of rumen ciliate based on an adaptation described by DEHORITY (I984). The modifying process includes: the replacement of two drops of brilliant green dye, for at least four hours, by three drops of lugol solution, for at least 15 minutes. It was made a comparative evaluation of these stainings. It was concluded that lugol solution can replace the brilliant green dye showing the following advantages: staining time reduction and subsequent speeding of sample processing; evidence of skeletal plates of entodiniomorphs making its identification easier; improved observation of small ciliates and inconspicuous structures; improved total counting and generic identification of the ciliates.Sociedade Brasileira de Zoologia1999-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751999000300011Revista Brasileira de Zoologia v.16 n.3 1999reponame:Revista Brasileira de Zoologia (Online)instname:Sociedade Brasileira de Zoologia (SBZ)instacron:SBZ10.1590/S0101-81751999000300011info:eu-repo/semantics/openAccessD'Agosto,MartaCarneiro,Maria Elisaeng2009-06-15T00:00:00Zoai:scielo:S0101-81751999000300011Revistahttp://calvados.c3sl.ufpr.br/ojs2/index.php/zooONGhttps://old.scielo.br/oai/scielo-oai.php||sbz@bio.ufpr.br1806-969X0101-8175opendoar:2009-06-15T00:00Revista Brasileira de Zoologia (Online) - Sociedade Brasileira de Zoologia (SBZ)false
dc.title.none.fl_str_mv Evaluation of lugol solution used for counting rumen ciliates
title Evaluation of lugol solution used for counting rumen ciliates
spellingShingle Evaluation of lugol solution used for counting rumen ciliates
D'Agosto,Marta
Rumen ciliate
quantitative evaluation
lugol solution
technique
title_short Evaluation of lugol solution used for counting rumen ciliates
title_full Evaluation of lugol solution used for counting rumen ciliates
title_fullStr Evaluation of lugol solution used for counting rumen ciliates
title_full_unstemmed Evaluation of lugol solution used for counting rumen ciliates
title_sort Evaluation of lugol solution used for counting rumen ciliates
author D'Agosto,Marta
author_facet D'Agosto,Marta
Carneiro,Maria Elisa
author_role author
author2 Carneiro,Maria Elisa
author2_role author
dc.contributor.author.fl_str_mv D'Agosto,Marta
Carneiro,Maria Elisa
dc.subject.por.fl_str_mv Rumen ciliate
quantitative evaluation
lugol solution
technique
topic Rumen ciliate
quantitative evaluation
lugol solution
technique
description The present study proposes a modification of the technique described by PURSER & MOIR (1959) for the quantitative evaluation of rumen ciliate based on an adaptation described by DEHORITY (I984). The modifying process includes: the replacement of two drops of brilliant green dye, for at least four hours, by three drops of lugol solution, for at least 15 minutes. It was made a comparative evaluation of these stainings. It was concluded that lugol solution can replace the brilliant green dye showing the following advantages: staining time reduction and subsequent speeding of sample processing; evidence of skeletal plates of entodiniomorphs making its identification easier; improved observation of small ciliates and inconspicuous structures; improved total counting and generic identification of the ciliates.
publishDate 1999
dc.date.none.fl_str_mv 1999-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751999000300011
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751999000300011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0101-81751999000300011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Zoologia
publisher.none.fl_str_mv Sociedade Brasileira de Zoologia
dc.source.none.fl_str_mv Revista Brasileira de Zoologia v.16 n.3 1999
reponame:Revista Brasileira de Zoologia (Online)
instname:Sociedade Brasileira de Zoologia (SBZ)
instacron:SBZ
instname_str Sociedade Brasileira de Zoologia (SBZ)
instacron_str SBZ
institution SBZ
reponame_str Revista Brasileira de Zoologia (Online)
collection Revista Brasileira de Zoologia (Online)
repository.name.fl_str_mv Revista Brasileira de Zoologia (Online) - Sociedade Brasileira de Zoologia (SBZ)
repository.mail.fl_str_mv ||sbz@bio.ufpr.br
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