Polimorfismo enzimático em populações de Melipona quadrifasciata anthidioides Lepeletier (Hymenoptera, Apidae, Meliponinae)

Detalhes bibliográficos
Autor(a) principal: Aidar,Davi S.
Data de Publicação: 2001
Outros Autores: Pagotto,Rubiane C., Contel,Eucleia P.B.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista Brasileira de Zoologia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752001000200018
Resumo: Them aim scope of this study is to characterize the enzymatic polymorphism found in the Melipona quadrifasciata Lepeletier, 1936 populations from Ribeirão Preto, São Paulo and Espírito Santo, Brazil and its hybrids. Samples from each colony (about 52) were prepared for starch gel electrophoresis in order to investigate the genetic variation of the following enzimes: esterase (EST), isocitrate dehydrogenase (IDH), malic enzyme (ME), phosphoglucomutase (PGM), superoxide desmutase (SOD), α-glycerophosphate dehydrogenase (αPGD), malate dehydrogenase (MDH), leucine aminopeptidase (LAP), hexokinase (HK) and phosphoglucose isomerase (PGI). The analysis showed that LAP and HK did not show enzymatic activity and EST showed two alleles(est-sand and est-f) while all the others were shown to be monomorphic. The allele EST-S showed a frequency of 82,6%.
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spelling Polimorfismo enzimático em populações de Melipona quadrifasciata anthidioides Lepeletier (Hymenoptera, Apidae, Meliponinae)Melipona quadrifasciata anthidioidespolymorphismelectrophoresisenzymestingless beesThem aim scope of this study is to characterize the enzymatic polymorphism found in the Melipona quadrifasciata Lepeletier, 1936 populations from Ribeirão Preto, São Paulo and Espírito Santo, Brazil and its hybrids. Samples from each colony (about 52) were prepared for starch gel electrophoresis in order to investigate the genetic variation of the following enzimes: esterase (EST), isocitrate dehydrogenase (IDH), malic enzyme (ME), phosphoglucomutase (PGM), superoxide desmutase (SOD), α-glycerophosphate dehydrogenase (αPGD), malate dehydrogenase (MDH), leucine aminopeptidase (LAP), hexokinase (HK) and phosphoglucose isomerase (PGI). The analysis showed that LAP and HK did not show enzymatic activity and EST showed two alleles(est-sand and est-f) while all the others were shown to be monomorphic. The allele EST-S showed a frequency of 82,6%.Sociedade Brasileira de Zoologia2001-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752001000200018Revista Brasileira de Zoologia v.18 n.2 2001reponame:Revista Brasileira de Zoologia (Online)instname:Sociedade Brasileira de Zoologia (SBZ)instacron:SBZ10.1590/S0101-81752001000200018info:eu-repo/semantics/openAccessAidar,Davi S.Pagotto,Rubiane C.Contel,Eucleia P.B.por2009-05-04T00:00:00Zoai:scielo:S0101-81752001000200018Revistahttp://calvados.c3sl.ufpr.br/ojs2/index.php/zooONGhttps://old.scielo.br/oai/scielo-oai.php||sbz@bio.ufpr.br1806-969X0101-8175opendoar:2009-05-04T00:00Revista Brasileira de Zoologia (Online) - Sociedade Brasileira de Zoologia (SBZ)false
dc.title.none.fl_str_mv Polimorfismo enzimático em populações de Melipona quadrifasciata anthidioides Lepeletier (Hymenoptera, Apidae, Meliponinae)
title Polimorfismo enzimático em populações de Melipona quadrifasciata anthidioides Lepeletier (Hymenoptera, Apidae, Meliponinae)
spellingShingle Polimorfismo enzimático em populações de Melipona quadrifasciata anthidioides Lepeletier (Hymenoptera, Apidae, Meliponinae)
Aidar,Davi S.
Melipona quadrifasciata anthidioides
polymorphism
electrophoresis
enzyme
stingless bees
title_short Polimorfismo enzimático em populações de Melipona quadrifasciata anthidioides Lepeletier (Hymenoptera, Apidae, Meliponinae)
title_full Polimorfismo enzimático em populações de Melipona quadrifasciata anthidioides Lepeletier (Hymenoptera, Apidae, Meliponinae)
title_fullStr Polimorfismo enzimático em populações de Melipona quadrifasciata anthidioides Lepeletier (Hymenoptera, Apidae, Meliponinae)
title_full_unstemmed Polimorfismo enzimático em populações de Melipona quadrifasciata anthidioides Lepeletier (Hymenoptera, Apidae, Meliponinae)
title_sort Polimorfismo enzimático em populações de Melipona quadrifasciata anthidioides Lepeletier (Hymenoptera, Apidae, Meliponinae)
author Aidar,Davi S.
author_facet Aidar,Davi S.
Pagotto,Rubiane C.
Contel,Eucleia P.B.
author_role author
author2 Pagotto,Rubiane C.
Contel,Eucleia P.B.
author2_role author
author
dc.contributor.author.fl_str_mv Aidar,Davi S.
Pagotto,Rubiane C.
Contel,Eucleia P.B.
dc.subject.por.fl_str_mv Melipona quadrifasciata anthidioides
polymorphism
electrophoresis
enzyme
stingless bees
topic Melipona quadrifasciata anthidioides
polymorphism
electrophoresis
enzyme
stingless bees
description Them aim scope of this study is to characterize the enzymatic polymorphism found in the Melipona quadrifasciata Lepeletier, 1936 populations from Ribeirão Preto, São Paulo and Espírito Santo, Brazil and its hybrids. Samples from each colony (about 52) were prepared for starch gel electrophoresis in order to investigate the genetic variation of the following enzimes: esterase (EST), isocitrate dehydrogenase (IDH), malic enzyme (ME), phosphoglucomutase (PGM), superoxide desmutase (SOD), α-glycerophosphate dehydrogenase (αPGD), malate dehydrogenase (MDH), leucine aminopeptidase (LAP), hexokinase (HK) and phosphoglucose isomerase (PGI). The analysis showed that LAP and HK did not show enzymatic activity and EST showed two alleles(est-sand and est-f) while all the others were shown to be monomorphic. The allele EST-S showed a frequency of 82,6%.
publishDate 2001
dc.date.none.fl_str_mv 2001-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752001000200018
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752001000200018
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv 10.1590/S0101-81752001000200018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Zoologia
publisher.none.fl_str_mv Sociedade Brasileira de Zoologia
dc.source.none.fl_str_mv Revista Brasileira de Zoologia v.18 n.2 2001
reponame:Revista Brasileira de Zoologia (Online)
instname:Sociedade Brasileira de Zoologia (SBZ)
instacron:SBZ
instname_str Sociedade Brasileira de Zoologia (SBZ)
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institution SBZ
reponame_str Revista Brasileira de Zoologia (Online)
collection Revista Brasileira de Zoologia (Online)
repository.name.fl_str_mv Revista Brasileira de Zoologia (Online) - Sociedade Brasileira de Zoologia (SBZ)
repository.mail.fl_str_mv ||sbz@bio.ufpr.br
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