Efeitos do acetato de chumbo em tecido ósseo de ratos

Detalhes bibliográficos
Autor(a) principal: Pastor, Fabio Alexandre Casarin
Data de Publicação: 2009
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFSCAR
Texto Completo: https://repositorio.ufscar.br/handle/ufscar/1307
Resumo: Lead is a ubiquitous pollutant in the environmental ecosystem which distribution is mainly anthropogenic, come from burning fossil fuels, mining and various industrial products. There is little knowledge of the involvement of this element on the bone metabolism. This study aimed to determine possible changes in adult rat bone, contaminated with lead acetate during the development phase, through the incorporation of lead in the tissue. Biometric, biophysical, and biomechanical bone parameters were analyzed. The animals were divided into 3 groups: control, 9 rats treated with saline 0.9% (0.1 ml/100g BM) and contaminated groups C-100 and C-200 with 10 animals each, poisoned with lead acetate (100 and 200 mg / kg BM, respectively), once per week for 8 weeks by gastric gavage method. The incorporation of lead was examined with scanning electron microscopy and the concentration of this element in bone tissue with atomic absorption spectrometry with flame. We calculated the bone volume, and from this parameter the bone density was determined. The biomechanical properties of femurs were obtained by three points bending test. Contamination with lead acetate promoted incorporation and increased the concentration of lead in bone for the two doses tested, however were not sufficient to cause changes in the somatic and bone development of these animals. The dose of 100 mg / kg BM was not enough to cause bone changes and the dose of 200 mg / kg BM caused reduced bone density and strength. Therefore, the results indicate the ability of this element in damage the quality of the bone and maybe, serve as a source for endogenous subsequent contamination.
id SCAR_06169dbacc67e088506eabcc90de72f7
oai_identifier_str oai:repositorio.ufscar.br:ufscar/1307
network_acronym_str SCAR
network_name_str Repositório Institucional da UFSCAR
repository_id_str 4322
spelling Pastor, Fabio Alexandre CasarinNonaka, Keico Okinohttp://genos.cnpq.br:12010/dwlattes/owa/prc_imp_cv_int?f_cod=K4781719U9http://lattes.cnpq.br/3130130830810690b38b427a-8452-4715-b545-0c4fba05198d2016-06-02T19:22:51Z2009-07-232016-06-02T19:22:51Z2009-04-09PASTOR, Fabio Alexandre Casarin. Efeitos do acetato de chumbo em tecido ósseo de ratos. 2009. 132 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de São Carlos, São Carlos, 2009.https://repositorio.ufscar.br/handle/ufscar/1307Lead is a ubiquitous pollutant in the environmental ecosystem which distribution is mainly anthropogenic, come from burning fossil fuels, mining and various industrial products. There is little knowledge of the involvement of this element on the bone metabolism. This study aimed to determine possible changes in adult rat bone, contaminated with lead acetate during the development phase, through the incorporation of lead in the tissue. Biometric, biophysical, and biomechanical bone parameters were analyzed. The animals were divided into 3 groups: control, 9 rats treated with saline 0.9% (0.1 ml/100g BM) and contaminated groups C-100 and C-200 with 10 animals each, poisoned with lead acetate (100 and 200 mg / kg BM, respectively), once per week for 8 weeks by gastric gavage method. The incorporation of lead was examined with scanning electron microscopy and the concentration of this element in bone tissue with atomic absorption spectrometry with flame. We calculated the bone volume, and from this parameter the bone density was determined. The biomechanical properties of femurs were obtained by three points bending test. Contamination with lead acetate promoted incorporation and increased the concentration of lead in bone for the two doses tested, however were not sufficient to cause changes in the somatic and bone development of these animals. The dose of 100 mg / kg BM was not enough to cause bone changes and the dose of 200 mg / kg BM caused reduced bone density and strength. Therefore, the results indicate the ability of this element in damage the quality of the bone and maybe, serve as a source for endogenous subsequent contamination.Chumbo é um poluente ubíquo no ecossistema cuja distribuição ambiental é principalmente antropogênica, oriunda da queima de combustíveis fósseis, mineração e fabricações industriais diversas. Há pouco conhecimento do envolvimento deste elemento sobre o metabolismo ósseo. O objetivo deste estudo foi estabelecer possíveis alterações ósseas em ratos adultos, contaminados com acetato de chumbo durante a fase de desenvolvimento, por meio da incorporação de chumbo neste tecido. Foram analisados parâmetros biométricos, biofísicos, e biomecânicos ósseos. Os animais foram divididos em 3 grupos: controle, com 9 ratos tratados com salina 0,9% (0,1 ml/100g MC) e contaminados C-100 e C-200 com 10 animais cada, intoxicados com acetato de chumbo (100 e 200 mg/kg MC, respectivamente), 1 vez por semana, por 8 semanas pelo método de gavagem gástrica. A incorporação de chumbo foi analisada com microscopia eletrônica de varredura e as concentrações deste elemento no tecido ósseo com espectrometria de absorção atômica com chama. Calculou-se o volume ósseo, e a partir deste parâmetro a densidade óssea foi determinada. As propriedades biomecânicas dos fêmures foram obtidas pelo teste de flexão a três pontos. A contaminação com acetato de chumbo promoveu incorporação e aumento da concentração de chumbo no tecido ósseo para as duas doses testadas, entretanto não foram suficientes para provocar alterações no desenvolvimento somático e ósseo destes animais. A dose de 100 mg/kg MC não foi suficiente para provocar alterações ósseas e a dose de 200 mg/kg MC provocou redução da densidade e resistência óssea. Portanto, os resultados apontam a capacidade deste elemento em prejudicar a qualidade do tecido ósseo e talvez servir como fonte endógena para contaminações subseqüentes.Universidade Federal de Sao Carlosapplication/pdfporUniversidade Federal de São CarlosPrograma Interinstitucional de Pós-Graduação em Ciências Fisiológicas - PIPGCFUFSCarBRTecido ósseoAcetato de chumboBiomecânica ósseaDensidade ósseaRatoBone tissueLead acetateBone biomechanicsBone densityRatCIENCIAS BIOLOGICAS::FISIOLOGIAEfeitos do acetato de chumbo em tecido ósseo de ratosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-1-1ebecdfb1-cdc6-42ca-b661-e018c16ea74cinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFSCARinstname:Universidade Federal de São Carlos (UFSCAR)instacron:UFSCARORIGINAL2339.pdfapplication/pdf646629https://repositorio.ufscar.br/bitstream/ufscar/1307/1/2339.pdfa664e5d6f2b2d647db3059c99759df5bMD51THUMBNAIL2339.pdf.jpg2339.pdf.jpgIM Thumbnailimage/jpeg6090https://repositorio.ufscar.br/bitstream/ufscar/1307/2/2339.pdf.jpg5ca0515e11759edd388276bbebda689bMD52ufscar/13072023-09-18 18:31:39.153oai:repositorio.ufscar.br:ufscar/1307Repositório InstitucionalPUBhttps://repositorio.ufscar.br/oai/requestopendoar:43222023-09-18T18:31:39Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)false
dc.title.por.fl_str_mv Efeitos do acetato de chumbo em tecido ósseo de ratos
title Efeitos do acetato de chumbo em tecido ósseo de ratos
spellingShingle Efeitos do acetato de chumbo em tecido ósseo de ratos
Pastor, Fabio Alexandre Casarin
Tecido ósseo
Acetato de chumbo
Biomecânica óssea
Densidade óssea
Rato
Bone tissue
Lead acetate
Bone biomechanics
Bone density
Rat
CIENCIAS BIOLOGICAS::FISIOLOGIA
title_short Efeitos do acetato de chumbo em tecido ósseo de ratos
title_full Efeitos do acetato de chumbo em tecido ósseo de ratos
title_fullStr Efeitos do acetato de chumbo em tecido ósseo de ratos
title_full_unstemmed Efeitos do acetato de chumbo em tecido ósseo de ratos
title_sort Efeitos do acetato de chumbo em tecido ósseo de ratos
author Pastor, Fabio Alexandre Casarin
author_facet Pastor, Fabio Alexandre Casarin
author_role author
dc.contributor.authorlattes.por.fl_str_mv http://lattes.cnpq.br/3130130830810690
dc.contributor.author.fl_str_mv Pastor, Fabio Alexandre Casarin
dc.contributor.advisor1.fl_str_mv Nonaka, Keico Okino
dc.contributor.advisor1Lattes.fl_str_mv http://genos.cnpq.br:12010/dwlattes/owa/prc_imp_cv_int?f_cod=K4781719U9
dc.contributor.authorID.fl_str_mv b38b427a-8452-4715-b545-0c4fba05198d
contributor_str_mv Nonaka, Keico Okino
dc.subject.por.fl_str_mv Tecido ósseo
Acetato de chumbo
Biomecânica óssea
Densidade óssea
Rato
topic Tecido ósseo
Acetato de chumbo
Biomecânica óssea
Densidade óssea
Rato
Bone tissue
Lead acetate
Bone biomechanics
Bone density
Rat
CIENCIAS BIOLOGICAS::FISIOLOGIA
dc.subject.eng.fl_str_mv Bone tissue
Lead acetate
Bone biomechanics
Bone density
Rat
dc.subject.cnpq.fl_str_mv CIENCIAS BIOLOGICAS::FISIOLOGIA
description Lead is a ubiquitous pollutant in the environmental ecosystem which distribution is mainly anthropogenic, come from burning fossil fuels, mining and various industrial products. There is little knowledge of the involvement of this element on the bone metabolism. This study aimed to determine possible changes in adult rat bone, contaminated with lead acetate during the development phase, through the incorporation of lead in the tissue. Biometric, biophysical, and biomechanical bone parameters were analyzed. The animals were divided into 3 groups: control, 9 rats treated with saline 0.9% (0.1 ml/100g BM) and contaminated groups C-100 and C-200 with 10 animals each, poisoned with lead acetate (100 and 200 mg / kg BM, respectively), once per week for 8 weeks by gastric gavage method. The incorporation of lead was examined with scanning electron microscopy and the concentration of this element in bone tissue with atomic absorption spectrometry with flame. We calculated the bone volume, and from this parameter the bone density was determined. The biomechanical properties of femurs were obtained by three points bending test. Contamination with lead acetate promoted incorporation and increased the concentration of lead in bone for the two doses tested, however were not sufficient to cause changes in the somatic and bone development of these animals. The dose of 100 mg / kg BM was not enough to cause bone changes and the dose of 200 mg / kg BM caused reduced bone density and strength. Therefore, the results indicate the ability of this element in damage the quality of the bone and maybe, serve as a source for endogenous subsequent contamination.
publishDate 2009
dc.date.available.fl_str_mv 2009-07-23
2016-06-02T19:22:51Z
dc.date.issued.fl_str_mv 2009-04-09
dc.date.accessioned.fl_str_mv 2016-06-02T19:22:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv PASTOR, Fabio Alexandre Casarin. Efeitos do acetato de chumbo em tecido ósseo de ratos. 2009. 132 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de São Carlos, São Carlos, 2009.
dc.identifier.uri.fl_str_mv https://repositorio.ufscar.br/handle/ufscar/1307
identifier_str_mv PASTOR, Fabio Alexandre Casarin. Efeitos do acetato de chumbo em tecido ósseo de ratos. 2009. 132 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de São Carlos, São Carlos, 2009.
url https://repositorio.ufscar.br/handle/ufscar/1307
dc.language.iso.fl_str_mv por
language por
dc.relation.confidence.fl_str_mv -1
-1
dc.relation.authority.fl_str_mv ebecdfb1-cdc6-42ca-b661-e018c16ea74c
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de São Carlos
dc.publisher.program.fl_str_mv Programa Interinstitucional de Pós-Graduação em Ciências Fisiológicas - PIPGCF
dc.publisher.initials.fl_str_mv UFSCar
dc.publisher.country.fl_str_mv BR
publisher.none.fl_str_mv Universidade Federal de São Carlos
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFSCAR
instname:Universidade Federal de São Carlos (UFSCAR)
instacron:UFSCAR
instname_str Universidade Federal de São Carlos (UFSCAR)
instacron_str UFSCAR
institution UFSCAR
reponame_str Repositório Institucional da UFSCAR
collection Repositório Institucional da UFSCAR
bitstream.url.fl_str_mv https://repositorio.ufscar.br/bitstream/ufscar/1307/1/2339.pdf
https://repositorio.ufscar.br/bitstream/ufscar/1307/2/2339.pdf.jpg
bitstream.checksum.fl_str_mv a664e5d6f2b2d647db3059c99759df5b
5ca0515e11759edd388276bbebda689b
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFSCAR - Universidade Federal de São Carlos (UFSCAR)
repository.mail.fl_str_mv
_version_ 1813715509941633024